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Elmlund, Louise
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Publikasjoner (8 av 8) Visa alla publikasjoner
Elmlund, L., Käck, C., Aastrup, T. & Nicholls, I. A. (2015). Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor. Sensors, 15(3), 5884-5894
Åpne denne publikasjonen i ny fane eller vindu >>Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor
2015 (engelsk)Inngår i: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 15, nr 3, s. 5884-5894Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Analytical methods founded upon whole cell-based assays are of importance in early stage drug development and in fundamental studies of biomolecular recognition. Here we have studied the binding of the monoclonal antibody trastuzumab to human epidermal growth factor receptor 2 (HER2) on human ovary adenocarcinoma epithelial cancer cells (SKOV3) using quartz crystal microbalance (QCM) technology. An optimized procedure for immobilizing the cells on the chip surface was established with respect to fixation procedure and seeding density. Trastuzumab binding to the cell decorated sensor surface was studied, revealing a mean dissociation constant, K-D, value of 7 +/- 1 nM (standard error of the mean). This study provides a new perspective on the affinity of the antibody-receptor complex presented a more natural context compared to purified receptors. These results demonstrate the potential for using whole cell-based QCM assay in drug development, the screening of HER2 selective antibody-based drug candidates, and for the study of biomolecular recognition. This real time, label free approach for studying interactions with target receptors present in their natural environment afforded sensitive and detailed kinetic information about the binding of the analyte to the target.

sted, utgiver, år, opplag, sider
MDPI, 2015
Emneord
quartz crystal microbalance, breast cancer, cell-based biosensor, Herceptin, trastuzumab, HER2
HSV kategori
Forskningsprogram
Naturvetenskap, Biomedicinsk vetenskap
Identifikatorer
urn:nbn:se:lnu:diva-37513 (URN)10.3390/s150305884 (DOI)000354160900066 ()25763651 (PubMedID)2-s2.0-84928680424 (Scopus ID)
Tilgjengelig fra: 2014-10-07 Laget: 2014-10-07 Sist oppdatert: 2018-11-16bibliografisk kontrollert
Elmlund, L., Söderberg, P., Suriyanarayanan, S. & Nicholls, I. A. (2014). A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety. Biosensors, 4(2), 137-149
Åpne denne publikasjonen i ny fane eller vindu >>A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
2014 (engelsk)Inngår i: Biosensors, ISSN 2079-6374, Vol. 4, nr 2, s. 137-149Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Phage display screening of a surface-immobilized adenine derivative led to the identification of a heptameric peptide with selectivity for adenine as demonstrated through quartz crystal microbalance (QCM) studies. The peptide demonstrated a concentration dependent affinity for an adeninyl moiety decorated surface (KD of 968 ± 53.3 μM), which highlights the power of piezoelectric sensing in the study of weak interactions. 

HSV kategori
Forskningsprogram
Kemi, Biokemi
Identifikatorer
urn:nbn:se:lnu:diva-37248 (URN)10.3390/bios4020137 (DOI)2-s2.0-84902317201 (Scopus ID)
Tilgjengelig fra: 2014-09-24 Laget: 2014-09-24 Sist oppdatert: 2018-11-02bibliografisk kontrollert
Elmlund, L., Suriyanarayanan, S., Wiklander, J. G., Aastrup, T. & Nicholls, I. A. (2014). Biotin selective polymer nano-films. Journal of Nanobiotechnology, 12, Article ID 8.
Åpne denne publikasjonen i ny fane eller vindu >>Biotin selective polymer nano-films
Vise andre…
2014 (engelsk)Inngår i: Journal of Nanobiotechnology, ISSN 1477-3155, E-ISSN 1477-3155, Vol. 12, artikkel-id 8Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: The interaction between biotin and avidin is utilized in a wide range of assay and diagnostic systems. A robust material capable of binding biotin should offer scope in the development of reusable assay materials and biosensor recognition elements. Results: Biotin-selective thin (3-5 nm) films have been fabricated on hexadecanethiol self assembled monolayer (SAM) coated Au/quartz resonators. The films were prepared based upon a molecular imprinting strategy where N, N'-methylenebisacrylamide and 2-acrylamido-2-methylpropanesulfonic acid were copolymerized and grafted to the SAM-coated surface in the presence of biotin methyl ester using photoinitiation with physisorbed benzophenone. The biotinyl moiety selectivity of the resonators efficiently differentiated biotinylated peptidic or carbohydrate structures from their native counterparts. Conclusions: Molecularly imprinted ultra thin films can be used for the selective recognition of biotinylated structures in a quartz crystal microbalance sensing platform. These films are stable for periods of at least a month. This strategy should prove of interest for use in other sensing and assay systems.

Emneord
Molecularly imprinted polymer, Biotinylated compounds, Photoinitiated graft co-polymerization, Quartz crystal microbalance (QCM) sensors, Sandwich-casting method
HSV kategori
Forskningsprogram
Kemi, Organisk kemi
Identifikatorer
urn:nbn:se:lnu:diva-34479 (URN)10.1186/1477-3155-12-8 (DOI)000335075000001 ()2-s2.0-84899157003 (Scopus ID)
Tilgjengelig fra: 2014-05-28 Laget: 2014-05-28 Sist oppdatert: 2020-03-20bibliografisk kontrollert
Elmlund, L. (2014). QCM-based sensing using biological and biomimetic interfaces. (Doctoral dissertation). Växjö: Linnaeus University Press
Åpne denne publikasjonen i ny fane eller vindu >>QCM-based sensing using biological and biomimetic interfaces
2014 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

The objective of this thesis was to explore novel approaches for studying molecular recognition at biological and biomimetic surfaces using the quartz crystal microbalance (QCM) biosensor technique. The first two papers focused on the synthesis and study of biotin selective polymer films prepared using the molecularly imprinted polymer (MIP) technique. Control over polymer structure is of importance for sensor reproducibility and sensitivity, and was addressed in Paper I where a simple strategy for fabricating uniform thin biotin imprinted polymer films was employed. In Paper II the binding of biotin moieties to thin (3-5 nm) biomimetic polymer films was examined and consequences for sensor performance discussed. The potential for using QCM as a tool for assessing the binding of small peptides derived from phage display screening was presented Paper III. Here, screening of a phage peptide library against immobilized adenine resulted in candidate peptides that were studied using this technique. In Paper IV a whole cell-based biosensor was developed for studying interactions with cell membrane-incorporated targets. Epithelial cancer cells, SKOV3, were attached to QCM sensor chips and the binding of the monoclonal antibody HerceptinTM was studied. This approach demonstrates the potential of using QCM to study binding to membrane-incorporated targets, an alternative to assays based upon immobilized receptor structures lacking their natural context.

sted, utgiver, år, opplag, sider
Växjö: Linnaeus University Press, 2014
Serie
Linnaeus University Dissertations ; 192/2014
Emneord
Quartz crystal microbalance, molecularly imprinted polymers, phage display, interactions, artificial receptors, recognition, selectivity
HSV kategori
Identifikatorer
urn:nbn:se:lnu:diva-37514 (URN)978-91-87925-20-7 (ISBN)
Disputas
2014-10-31, N2007, Smålandsgatan 26B, Kalmar, 09:30 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2014-10-08 Laget: 2014-10-07 Sist oppdatert: 2014-10-08bibliografisk kontrollert
Elmlund, L. (2013). Cell-Antibody Interactions Studied Using HerceptinTM (trastuzumab) binding to SKOV-3 epithelial cancer cells characterized by Attana™ Quartz Crystal Microbalance Technology. In: : . Paper presented at IBC's 24th Annual International Conference: Antibody Engineering & Therapeutics, December 8-12, 2013, Huntington Beach, CA.
Åpne denne publikasjonen i ny fane eller vindu >>Cell-Antibody Interactions Studied Using HerceptinTM (trastuzumab) binding to SKOV-3 epithelial cancer cells characterized by Attana™ Quartz Crystal Microbalance Technology
2013 (engelsk)Konferansepaper, Poster (with or without abstract) (Annet vitenskapelig)
HSV kategori
Forskningsprogram
Naturvetenskap, Biomedicinsk vetenskap
Identifikatorer
urn:nbn:se:lnu:diva-37247 (URN)
Konferanse
IBC's 24th Annual International Conference: Antibody Engineering & Therapeutics, December 8-12, 2013, Huntington Beach, CA
Tilgjengelig fra: 2014-10-07 Laget: 2014-09-24 Sist oppdatert: 2014-12-09bibliografisk kontrollert
Huang, S., Nilsson, P. H., Sandholm, K., Elmlund, L., Nicholls, I. A. & Nilsson Ekdahl, K. (2012). Regulation of complement in whole blood by heparin molecularly imprinted polymer particles. Immunobiology, 217(11), 1199-1199
Åpne denne publikasjonen i ny fane eller vindu >>Regulation of complement in whole blood by heparin molecularly imprinted polymer particles
Vise andre…
2012 (engelsk)Inngår i: Immunobiology, ISSN 0171-2985, E-ISSN 1878-3279, Vol. 217, nr 11, s. 1199-1199Artikkel i tidsskrift, Meeting abstract (Annet vitenskapelig) Published
HSV kategori
Forskningsprogram
Biomedicinsk vetenskap, Immunologi
Identifikatorer
urn:nbn:se:lnu:diva-23357 (URN)10.1016/j.imbio.2012.08.200 (DOI)000311187800212 ()
Tilgjengelig fra: 2013-01-09 Laget: 2013-01-09 Sist oppdatert: 2017-12-06bibliografisk kontrollert
Oskarsson, L., Söderberg, P. & Nicholls, I. A. (2010). Towards Phage Display-Derived Synthetic Receptors for Adenine. In: : . Paper presented at 22:nd Organic Chemistry Days (Organikerdagarna), Uppsala.
Åpne denne publikasjonen i ny fane eller vindu >>Towards Phage Display-Derived Synthetic Receptors for Adenine
2010 (engelsk)Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

The expression of peptides on phage coat proteins via genetic engineering was first achieved in 1985 [1] and was followed by the construction of phage display combinatorial libraries in 1990 [2].  Phage display combinatorial libraries have been used not only to find peptides that interact with various biomolecules such as receptors, antibodies, enzyme active sites, and protein surfaces, but also to find peptides with affinity for metals and plastics and with function in non-biological environments [3].

In this study, we have challenged a cyclic heptamer phage display library with surfaces adenine functionalised surfaces with the aim of identifying peptide motifs selective for adenine. The synthesis and characterisation of surfaces, and results from phage display screening shall be presented.

 

 

[1] Smith, G.P. Science (1985) 228, 1315-1317.

[2] Scott, J.K.; Smith, G.P. Science (1990) 249, 386-390.

[3] Olofsson, L.; Söderberg, P.; Ankarloo, J.; Nicholls, I.A. J. Mol. Recognit. (2008) 19, 18-25.

HSV kategori
Forskningsprogram
Naturvetenskap, Organisk kemi
Identifikatorer
urn:nbn:se:lnu:diva-6714 (URN)
Konferanse
22:nd Organic Chemistry Days (Organikerdagarna), Uppsala
Tilgjengelig fra: 2010-07-07 Laget: 2010-07-07 Sist oppdatert: 2015-04-16bibliografisk kontrollert
Elmlund, L., Suriyanarayanan, S., Wiklander, J. & Nicholls, I. A.Simple Strategy for Steering Polymer Film Formation on QCM Sensor Surfaces.
Åpne denne publikasjonen i ny fane eller vindu >>Simple Strategy for Steering Polymer Film Formation on QCM Sensor Surfaces
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Forskningsprogram
Naturvetenskap, Organisk kemi
Identifikatorer
urn:nbn:se:lnu:diva-37250 (URN)
Tilgjengelig fra: 2014-10-07 Laget: 2014-09-24 Sist oppdatert: 2020-03-20bibliografisk kontrollert
Organisasjoner