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Legrand, Catherine, ProfessorORCID iD iconorcid.org/0000-0001-7155-3604
Publications (10 of 107) Show all publications
Sörenson, E., Bertos-Fortis, M., Farnelid, H., Kremp, A., Kruget, K., Lindehoff, E. & Legrand, C. (2019). Consistency in microbiomes in cultures of Alexandrium species isolated from brackish and marine waters. Environmental Microbiology Reports, 11(3), 425-433
Open this publication in new window or tab >>Consistency in microbiomes in cultures of Alexandrium species isolated from brackish and marine waters
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2019 (English)In: Environmental Microbiology Reports, ISSN 1758-2229, E-ISSN 1758-2229, Vol. 11, no 3, p. 425-433Article in journal (Refereed) Published
Abstract [en]

Phytoplankton and bacteria interactions have a significant role in aquatic ecosystem functioning. Associations can range from mutualistic to parasitic, shaping biogeochemical cycles and having a direct influence on phytoplankton growth. How variations in phenotype and sampling location, affect the phytoplankton microbiome is largely unknown. A high‐resolution characterization of the bacterial community in cultures of the dinoflagellate Alexandrium was performed on strains isolated from different geographical locations and at varying anthropogenic impact levels. Microbiomes of Baltic Sea Alexandrium ostenfeldii isolates were dominated by Betaproteobacteria and were consistent over phenotypic and genotypic Alexandrium strain variation, resulting in identification of an A. ostenfeldii core microbiome. Comparisons with in situ bacterial communities showed that taxa found in this A. ostenfeldii core were specifically associated to dinoflagellate dynamics in the Baltic Sea. Microbiomes of Alexandrium tamarense and minutum, isolated from the Mediterranean Sea, differed from those of A. ostenfeldii in bacterial diversity and composition but displayed high consistency, and a core set of bacterial taxa was identified. This indicates that Alexandrium isolates with diverse phenotypes host predictable, species‐specific, core microbiomes reflecting the abiotic conditions from which they were isolated. These findings enable in‐depth studies of potential interactions occurring between Alexandrium and specific bacterial taxa.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2019
Keywords
Algae, Bacteria, Interactions, Microbiome, Baltic Sea, Alexandrium, Alger, Bakterier, Interaktioner, Microbiom, Östersjön, Alexandrium
National Category
Ecology Microbiology
Research subject
Ecology, Microbiology; Ecology, Aquatic Ecology
Identifiers
urn:nbn:se:lnu:diva-81534 (URN)10.1111/1758-2229.12736 (DOI)000468000600014 ()30672139 (PubMedID)2-s2.0-85062772299 (Scopus ID)
Projects
EcoChange
Funder
Swedish Research Council FormasEU, European Research Council, 659453EU, Horizon 2020, 659453Carl Tryggers foundation , 14:283
Available from: 2019-04-01 Created: 2019-04-01 Last updated: 2019-08-29Bibliographically approved
Bunse, C., Israelsson, S., Baltar, F., Bertos-Fortis, M., Fridolfsson, E., Legrand, C., . . . Pinhassi, J. (2019). High Frequency Multi-Year Variability in Baltic Sea Microbial Plankton Stocks and Activities. Frontiers in Microbiology, 9, Article ID 3296.
Open this publication in new window or tab >>High Frequency Multi-Year Variability in Baltic Sea Microbial Plankton Stocks and Activities
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2019 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 9, article id 3296Article in journal (Refereed) Published
Abstract [en]

Marine bacterioplankton are essential in global nutrient cycling and organic matter turnover. Time-series analyses, often at monthly sampling frequencies, have established the paramount role of abiotic and biotic variables in structuring bacterioplankton communities and productivities. However, fine-scale seasonal microbial activities, and underlying biological principles, are not fully understood. We report results from four consecutive years of high-frequency time-series sampling in the Baltic Proper. Pronounced temporal dynamics in most investigated microbial variables were observed, including bacterial heterotrophic production, plankton biomass, extracellular enzyme activities, substrate uptake rate constants of glucose, pyruvate, acetate, amino acids, and leucine, as well as nutrient limitation bioassays. Spring blooms consisting of diatoms and dinoflagellates were followed by elevated bacterial heterotrophic production and abundances. During summer, bacterial productivity estimates increased even further, coinciding with an initial cyanobacterial bloom in early July. However, bacterial abundances only increased following a second cyanobacterial bloom, peaking in August. Uptake rate constants for the different measured carbon compounds varied seasonally and inter-annually and were highly correlated to bacterial productivity estimates, temperature, and cyanobacterial abundances. Further, we detected nutrient limitation in response to environmental conditions in a multitude of microbial variables, such as elevated productivities in nutrient bioassays, changes in enzymatic activities, or substrate preferences. Variations among biotic variables often occurred on time scales of days to a few weeks, yet often spanning several sampling occasions. Such dynamics might not have been captured by sampling at monthly intervals, as compared to more predictable transitions in abiotic variables such as temperature or nutrient concentrations. Our study indicates that high resolution analyses of microbial biomass and productivity parameters can help out in the development of biogeochemical and food web models disentangling the microbial black box.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019
Keywords
marine bacteria, phytoplankton, cyanobacteria, production, substrate uptake, enzyme activity, biogeochemistry
National Category
Microbiology Ecology
Research subject
Ecology, Microbiology; Ecology, Microbiology
Identifiers
urn:nbn:se:lnu:diva-80150 (URN)10.3389/fmicb.2018.03296 (DOI)000455948100001 ()2-s2.0-85064405301 (Scopus ID)
Available from: 2019-02-05 Created: 2019-02-05 Last updated: 2019-08-29Bibliographically approved
Olofsson, M., Lindehoff, E. & Legrand, C. (2019). Production stability and biomass quality in microalgal cultivation: contribution of community dynamics. Engineering in Life Sciences, 19(5), 330-340
Open this publication in new window or tab >>Production stability and biomass quality in microalgal cultivation: contribution of community dynamics
2019 (English)In: Engineering in Life Sciences, ISSN 1618-0240, E-ISSN 1618-2863, Vol. 19, no 5, p. 330-340Article in journal (Refereed) Published
Abstract [en]

The prospect of using constructed communities of microalgae in algal cultivation wasconfirmed in this study. Three constructed communities of diatoms (Diatom), greenalgae (Green) and cyanobacteria (Cyano), were each mixed with a natural communityof microalgae as baseline. The communities were cultivated in batch and semicontinuousmode and fed CO2 or cement flue gas (12-15 % CO2). Diatom had thehighest growth rate but Green had the highest yield. Dynamic changes in thecommunity composition occurred from start through batch to semi-steady state. Greenalgae were the most competitive group during the experiment. Euglenoids wererecruited from scarce species in the natural community and became a large part of thebiomass in semi-steady state in all communities. High temporal and yield stabilitywas demonstrated in all communities during semi-steady state. Biochemicalcomposition (lipids, proteins and carbohydrates) was similar for the threecommunities with lipids ranging 14-26 % of dry weight (DW), proteins (15-28 %DW) and carbohydrates (9-23 % DW). Filamentous cyanobacteria were outcompetedearly in the experiment. However, their minute presence in Cyano associated withhigher lipid and lower carbohydrates compared to Diatom and Green, suggesting theimportance of chemical interactions among microorganisms. Our results indicate thatculture functions (stability, biomass quality) were maintained while dynamic changesoccurred in community composition. We propose that a multi-species communityapproach can aid sustainability in microalgal cultivation, through complementary useof resources and higher culture stability. Local environmental conditions,complementary microalgal traits, and interactions among functional groups (algae,bacteria) should be considered in community design where natural succession andcrop rotation will likely provide stability for commercial-scale algal cultivation.

Place, publisher, year, edition, pages
Hoboken, NJ: John Wiley & Sons, 2019
Keywords
Microalgae, multi-species communities, production stability, algal cultivation, biomass composition, flue gas
National Category
Biological Sciences Ecology
Research subject
Ecology, Aquatic Ecology; Chemistry, Biotechnology; Environmental Science, Environmental technology
Identifiers
urn:nbn:se:lnu:diva-46511 (URN)10.1002/elsc.201900015 (DOI)000472189900001 ()2-s2.0-85063427279 (Scopus ID)
Available from: 2015-09-28 Created: 2015-09-28 Last updated: 2019-07-17Bibliographically approved
Fridolfsson, E., Bunse, C., Legrand, C., Lindehoff, E., Majaneva, S. & Hylander, S. (2019). Seasonal variation and species-specific concentrations of the essential vitamin B₁ (thiamin) in zooplankton and seston. Marine Biology, 166(6), 1-13, Article ID 70.
Open this publication in new window or tab >>Seasonal variation and species-specific concentrations of the essential vitamin B₁ (thiamin) in zooplankton and seston
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2019 (English)In: Marine Biology, ISSN 0025-3162, E-ISSN 1432-1793, Vol. 166, no 6, p. 1-13, article id 70Article in journal (Refereed) Published
Abstract [en]

Thiamin (vitamin B1) is mainly produced by bacteria and phytoplankton and then transferred to zooplankton and higher trophic levels but knowledge on the dynamics of these processes in aquatic ecosystems is lacking. Hence, the seasonal variation in thiamin content was assessed in field samples of copepods and in pico-, nano- and micro-plankton of two size classes (0.7–3 µm and > 3 µm) collected monthly in the Baltic Sea during 3 years and in the Skagerrak during 1 year. Copepods exhibited species-specific concentrations of thiamin and Acartia sp. had the highest carbon-specific thiamin content, at both locations. Even members of the same genus, but from different systems contained different levels of thiamin, with higher thiamin content per specimen in copepods from the Skagerrak compared to congeners from the Baltic Sea. Furthermore, our results show that the small plankton (0.7–3 µm) had a higher carbon-specific thiamin content compared to the large (> 3 µm). Additionally, there was a large seasonal variation and thiamin content was highly correlated comparing the two size fractions. Finally, there was an overall positive correlation between thiamin content in copepods and plankton. However, for periods of high thiamin content in the two size fractions, this correlation was negative. This suggests a decoupling between thiamin availability in pico-, nano- and micro-plankton and zooplankton in the Baltic Sea. Knowledge about concentrations of this essential micronutrient in the aquatic food web is limited and this study constitutes a foundation for further understanding the dynamics of thiamin in aquatic environments.

Place, publisher, year, edition, pages
New York, NY: Springer, 2019
National Category
Ecology
Research subject
Ecology, Aquatic Ecology
Identifiers
urn:nbn:se:lnu:diva-82081 (URN)10.1007/s00227-019-3520-6 (DOI)000467561000005 ()2-s2.0-85065572171 (Scopus ID)
Available from: 2019-04-23 Created: 2019-04-23 Last updated: 2019-08-29Bibliographically approved
Broman, E., Li, L., Fridlund, J., Svensson, F., Legrand, C. & Dopson, M. (2019). Spring and Late Summer Phytoplankton Biomass Impact on the Coastal Sediment Microbial Community Structure. Microbial Ecology (2), 288-303
Open this publication in new window or tab >>Spring and Late Summer Phytoplankton Biomass Impact on the Coastal Sediment Microbial Community Structure
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2019 (English)In: Microbial Ecology, ISSN 0095-3628, E-ISSN 1432-184X, no 2, p. 288-303Article in journal (Refereed) Published
Abstract [en]

Two annual Baltic Sea phytoplankton blooms occur in spring and summer. The bloom intensity is determined by nutrient concentrations in the water, while the period depends on weather conditions. During the course of the bloom, dead cells sink to the sediment where their degradation consumes oxygen to create hypoxic zones (< 2 mg/L dissolved oxygen). These zones prevent the establishment of benthic communities and may result in fish mortality. The aim of the study was to determine how the spring and autumn sediment chemistry and microbial community composition changed due to degradation of diatom or cyanobacterial biomass, respectively. Results from incubation of sediment cores showed some typical anaerobic microbial processes after biomass addition such as a decrease in NO2 + NO3 in the sediment surface (0–1 cm) and iron in the underlying layer (1–2 cm). In addition, an increase in NO2 + NO3 was observed in the overlying benthic water in all amended and control incubations. The combination of NO2 + NO3 diffusion plus nitrification could not account for this increase. Based on 16S rRNA gene sequences, the addition of cyanobacterial biomass during autumn caused a large increase in ferrous iron-oxidizing archaea while diatom biomass amendment during spring caused minor changes in the microbial community. Considering that OTUs sharing lineages with acidophilic microorganisms had a high relative abundance during autumn, it was suggested that specific niches developed in sediment microenvironments. These findings highlight the importance of nitrogen cycling and early microbial community changes in the sediment due to sinking phytoplankton before potential hypoxia occurs.

Place, publisher, year, edition, pages
Springer, 2019
National Category
Ecology Microbiology
Research subject
Ecology, Microbiology
Identifiers
urn:nbn:se:lnu:diva-76944 (URN)10.1007/s00248-018-1229-6 (DOI)000460479100002 ()2-s2.0-85049948034 (Scopus ID)
Available from: 2018-07-18 Created: 2018-07-18 Last updated: 2019-08-29Bibliographically approved
Legrand, C. (2018). Algoland: industry and ecology together. In: Presented at the 1st Nordic Algae Symposium 2018 (NAS18), Helsinki, Finland, January 31, 2018: . Paper presented at The 1st Nordic Algae Symposium 2018 (NAS18), Helsinki, Finland, January 31, 2018.
Open this publication in new window or tab >>Algoland: industry and ecology together
2018 (English)In: Presented at the 1st Nordic Algae Symposium 2018 (NAS18), Helsinki, Finland, January 31, 2018, 2018Conference paper, Oral presentation only (Other academic)
National Category
Ecology
Research subject
Ecology, Aquatic Ecology
Identifiers
urn:nbn:se:lnu:diva-73792 (URN)
Conference
The 1st Nordic Algae Symposium 2018 (NAS18), Helsinki, Finland, January 31, 2018
Projects
AlgolandEcochange
Available from: 2018-05-03 Created: 2018-05-03 Last updated: 2018-05-31Bibliographically approved
Legrand, C. (2018). Algoland Workshop: business models. In: Algoland 2030 Workshop, Kalmar, Sweden, April 24, 2018: . Paper presented at Algoland 2030 Workshop, Kalmar, Sweden, April 24, 2018.
Open this publication in new window or tab >>Algoland Workshop: business models
2018 (English)In: Algoland 2030 Workshop, Kalmar, Sweden, April 24, 2018, 2018Conference paper, Oral presentation only (Other academic)
National Category
Ecology
Research subject
Ecology, Aquatic Ecology
Identifiers
urn:nbn:se:lnu:diva-73947 (URN)
Conference
Algoland 2030 Workshop, Kalmar, Sweden, April 24, 2018
Projects
AlgolandEcoChange
Note

Algoland är projektet där akvatisk forskning tillsammans med kompetens från industrin tarfram innovativa, hållbara lösningar som minskar utsläpp av koldioxid och näringsämnen. Samtidigtproduceras en värdefull biomassa som kan användas som djurfoder eller biobränslen.För att ta nästa steg i Algoland projektet, bjuder Catherine Legrand och forskare inom Algoland in till en workshop där vi tillsammans blickar in iframtiden.

Målet är att ta fram ett eller flera erbjudanden baserade på Algoland för att ta konceptet ett steg närmare näringslivet. Detta kan hjälpa att identifiera erbjudanden, produkter, nyaforskningsmöjligheter samt bana vägen för att Algoland ska komma till ännu större nytta i samhälletsom en ekonomiskt, socialt och miljömässigt hållbar verksamhet.

Datum: tisdag, 24 april Tid: 10.00 - 15.00 (inklusive lunch)Plats: Villa Solbacken, Svensknabben, Kalmar

Available from: 2018-05-04 Created: 2018-05-04 Last updated: 2018-05-14Bibliographically approved
Hultman, B. (2018). Hållbara transporter nästa mål för Linné. Barometern OT, 16 April, pp. 8
Open this publication in new window or tab >>Hållbara transporter nästa mål för Linné
2018 (Swedish)In: Barometern OT, Vol. 16 April, p. 8-Article in journal, News item (Other (popular science, discussion, etc.)) Published
Keywords
environmentally certified, university, sustainability, sustainable transport, sweden, kalmar, växjö, public work, administrative studies, miljö, hållbarhet, samarbete, transport, miljöcertifieringen
National Category
Environmental Sciences Public Administration Studies
Research subject
Natural Science, Environmental Science
Identifiers
urn:nbn:se:lnu:diva-74360 (URN)
Available from: 2018-05-15 Created: 2018-05-15 Last updated: 2018-07-20Bibliographically approved
Andersson, A., Brugel, S., Paczkowska, J., Rowe, O. F., Figueroa, D., Kratzer, S. & Legrand, C. (2018). Influence of allochthonous dissolved organic matter on pelagic basal production in a northerly estuary. Estuarine, Coastal and Shelf Science, 204, 225-235
Open this publication in new window or tab >>Influence of allochthonous dissolved organic matter on pelagic basal production in a northerly estuary
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2018 (English)In: Estuarine, Coastal and Shelf Science, ISSN 0272-7714, E-ISSN 1096-0015, Vol. 204, p. 225-235Article in journal (Refereed) Published
Abstract [en]

Phytoplankton and heterotrophic bacteria are key groups at the base of aquatic food webs. In estuaries receiving riverine water with a high content of coloured allochthonous dissolved organic matter (ADOM), phytoplankton primary production may be reduced, while bacterial production is favoured. We tested this hypothesis by performing a field study in a northerly estuary receiving nutrient-poor, ADOM-rich riverine water, and analyzing results using multivariate statistics. Throughout the productive season, and especially during the spring river flush, the production and growth rate of heterotrophic bacteria were stimulated by the riverine inflow of dissolved organic carbon (DOC). In contrast, primary production and photosynthetic efficiency (i.e. phytoplankton growth rate) were negatively affected by DOC. Primary production related positively to phosphorus, which is the limiting nutrient in the area. In the upper estuary where DOC concentrations were the highest, the heterotrophic bacterial production constituted almost 100% of the basal production (sum of primary and bacterial production) during spring, while during summer the primary and bacterial production were approximately equal. Our study shows that riverine DOC had a strong negative influence on coastal phytoplankton production, likely due to light attenuation. On the other hand DOC showed a positive influence on bacterial production since it represents a supplementary food source. Thus, in boreal regions where climate change will cause increased river inflow to coastal waters, the balance between phytoplankton and bacterial production is likely to be changed, favouring bacteria. The pelagic food web structure and overall productivity will in turn be altered. (C) 2018 The Authors. Published by Elsevier Ltd.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Primary and bacterial production, Coastal areas, Estuary, Allochthonous dissolved organic matter, Northern Baltic Sea
National Category
Biological Sciences
Research subject
Ecology, Aquatic Ecology
Identifiers
urn:nbn:se:lnu:diva-76463 (URN)10.1016/j.ecss.2018.02.032 (DOI)000429757300020 ()2-s2.0-85043270673 (Scopus ID)
Available from: 2018-07-10 Created: 2018-07-10 Last updated: 2019-08-29Bibliographically approved
Berner, C., Bertos-Fortis, M., Pinhassi, J. & Legrand, C. (2018). Response of Microbial Communities to Changing Climate Conditions During Summer Cyanobacterial Blooms in the Baltic Sea. Frontiers in Microbiology, 9, Article ID 1562.
Open this publication in new window or tab >>Response of Microbial Communities to Changing Climate Conditions During Summer Cyanobacterial Blooms in the Baltic Sea
2018 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 9, article id 1562Article in journal (Refereed) Published
Abstract [en]

Frequencies and biomass of Baltic Sea cyanobacterial blooms are expected to be higher in future climate conditions, but also of longer duration as a result of increased sea surface temperature. Concurrently, climate predictions indicate a reduced salinity in the Baltic Sea. These climate-driven changes are expected to alter not solely the phytoplankton community but also the role of microbial communities for nutrient remineralization. Here, we present the response of summer plankton communities (filamentous cyanobacteria, picocyanobacteria, and heterotrophic bacteria) to the interplay of increasing temperature (from 16 to 18 degrees C and 20 degrees C) and reduced salinity (from salinity 6.9 to 5.9) in the Baltic Proper (NW Gotland Sea) using a microcosm approach. Warmer temperatures led to an earlier peak of cyanobacterial biomass, while yields were reduced. These conditions caused a decrease of nitrogen-fixers (Dolichospermum sp.) biomass, while non nitrogen-fixers (Pseudanabaena sp.) increased. Salinity reduction did not affect cyanobacterial growth nor community composition. Among heterotrophic bacteria, Actinobacteria showed preference for high temperature, while Gammaproteobacteria thrived at in situ temperature. Heterotrophic bacteria community changed drastically at lower salinity and resembled communities at high temperature. Picocyanobacteria and heterotrophic bacterial biomass had a pronounced increase associated with the decay of filamentous cyanobacteria. This suggests that shifts in community composition of heterotrophic bacteria are influenced both directly by abiotic factors (temperature and salinity) and potentially indirectly by cyanobacteria. Our findings suggest that at warmer temperature, lower yield of photosynthetic cyanobacteria combined with lower proportion of nitrogen-fixers in the community could result in lower carbon export to the marine food web with consequences for the decomposer community of heterotrophic bacteria.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2018
Keywords
microscopy, 16S rRNA, cyanobacteria, heterotrophic bacteria, biomass, summer bloom, Baltic Sea, climate change
National Category
Microbiology Ecology
Research subject
Ecology, Microbiology
Identifiers
urn:nbn:se:lnu:diva-77393 (URN)10.3389/fmicb.2018.01562 (DOI)000439753100001 ()30090087 (PubMedID)2-s2.0-85050485397 (Scopus ID)
Available from: 2018-08-29 Created: 2018-08-29 Last updated: 2019-08-29Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-7155-3604

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