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Danielsson, T., Carlsson, J., Schreyer, H., Ahnesjö, J., ten Siethoff, L., Ragnarsson, T., . . . Bergman, P. (2017). Blood biomarkers in male and female participants after an Ironman-distance triathlon. PLoS ONE, 12(6), 1-9, Article ID e0179324.
Open this publication in new window or tab >>Blood biomarkers in male and female participants after an Ironman-distance triathlon
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2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 6, p. 1-9, article id e0179324Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: While overall physical activity is clearly associated with a better short-term and long-term health, prolonged strenuous physical activity may result in a rise in acute levels of blood-biomarkers used in clinical practice for diagnosis of various conditions or diseases. In this study, we explored the acute effects of a full Ironman-distance triathlon on biomarkers related to heart-, liver-, kidney- and skeletal muscle damage immediately post-race and after one week's rest. We also examined if sex, age, finishing time and body composition influenced the post-race values of the biomarkers.

METHODS: A sample of 30 subjects was recruited (50% women) to the study. The subjects were evaluated for body composition and blood samples were taken at three occasions, before the race (T1), immediately after (T2) and one week after the race (T3). Linear regression models were fitted to analyse the independent contribution of sex and finishing time controlled for weight, body fat percentage and age, on the biomarkers at the termination of the race (T2). Linear mixed models were fitted to examine if the biomarkers differed between the sexes over time (T1-T3).

RESULTS: Being male was a significant predictor of higher post-race (T2) levels of myoglobin, CK, and creatinine levels and body weight was negatively associated with myoglobin. In general, the models were unable to explain the variation of the dependent variables. In the linear mixed models, an interaction between time (T1-T3) and sex was seen for myoglobin and creatinine, in which women had a less pronounced response to the race.

CONCLUSION: Overall women appear to tolerate the effects of prolonged strenuous physical activity better than men as illustrated by their lower values of the biomarkers both post-race as well as during recovery.

Place, publisher, year, edition, pages
PLOS, 2017
National Category
Sport and Fitness Sciences
Research subject
Social Sciences, Sport Science
Identifiers
urn:nbn:se:lnu:diva-65765 (URN)10.1371/journal.pone.0179324 (DOI)000403274700028 ()28609447 (PubMedID)2-s2.0-85020735267 (Scopus ID)
Available from: 2017-06-22 Created: 2017-06-22 Last updated: 2019-09-06Bibliographically approved
Lard, M., ten Siethoff, L., Kumar, S., Persson, M., te Kronnie, G., Månsson, A. & Linke, H. (2015). Nano-structuring for molecular motor control. In: Baldassare Di Bartolo, John Collins, Luciano Silvestri (Ed.), Nano-Structures for Optics and Photonics: Optical Strategies for Enhancing Sensing, Imaging, Communication and Energy Conversion. Paper presented at NATO Advanced Study Institute on Nano-Structures for Optics and Photonics: Optical Strategies for Enhancing, Sensing, Imaging, Communication, and Energy Conversion. Erice, Sicily, Italy, 4–19 July 2013 (pp. 459-459). Springer
Open this publication in new window or tab >>Nano-structuring for molecular motor control
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2015 (English)In: Nano-Structures for Optics and Photonics: Optical Strategies for Enhancing Sensing, Imaging, Communication and Energy Conversion / [ed] Baldassare Di Bartolo, John Collins, Luciano Silvestri, Springer, 2015, p. 459-459Conference paper, Published paper (Refereed)
Abstract [en]

The interaction of self-propelled biological molecular-motors and cytoskeletal filaments holds relevance for a variety of applications such as biosensing, drug screening, diagnostics and biocomputation. The use of these systems for lab-on-a-chip biotechnology applications shows potential for replacement of microfluidic flow by active, molecular-motor driven transport of filaments. The ability to control, confine and detect motile objects in such a system is possible by development of nanostructured surfaces for on-chip applications and fundamental studies of molecular-motors. Here we describe the localized detection (Lard et al., Sci Rep 3:1092, 2013) and fast transport of actin filaments by myosin molecular-motors (Lard et al., Biosens Biolectron 48(0):145–152, 2013), inserted within nanostructures, as a method for biocomputation and molecular concentration. These results include extensive myosin driven concentration of actin filaments on a miniaturized detector, of relevance for use of molecular-motors in a diagnostics platform. Also, we discuss the local enhancement of the fluorescence signal of filaments, relevant for use in a biocomputation device where tracking of potentially thousands of motile objects is of primary significance.

Place, publisher, year, edition, pages
Springer, 2015
Series
NATO Science for Peace and Security Series B: Physics and Biophysics, ISSN 1874-6500
National Category
Nano Technology Biochemistry and Molecular Biology
Research subject
Natural Science, Chemistry
Identifiers
urn:nbn:se:lnu:diva-55335 (URN)10.1007/978-94-017-9133-5_28 (DOI)2-s2.0-84943311770 (Scopus ID)
Conference
NATO Advanced Study Institute on Nano-Structures for Optics and Photonics: Optical Strategies for Enhancing, Sensing, Imaging, Communication, and Energy Conversion. Erice, Sicily, Italy, 4–19 July 2013
Available from: 2016-08-11 Created: 2016-08-10 Last updated: 2017-04-21Bibliographically approved
Lard, M., ten Siethoff, L., Generosi, J., Persson, M., Linke, H. & Månsson, A. (2015). Nanowire-Imposed Geometrical Control in Studies of Actomyosin Motor Function. IEEE Transactions on Nanobioscience, 14(3), 289-297
Open this publication in new window or tab >>Nanowire-Imposed Geometrical Control in Studies of Actomyosin Motor Function
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2015 (English)In: IEEE Transactions on Nanobioscience, ISSN 1536-1241, E-ISSN 1558-2639, Vol. 14, no 3, p. 289-297Article in journal (Refereed) Published
Abstract [en]

Recently, molecular motor gliding assays with actin and myosin from muscle have been realized on semiconductor nanowires coated with Al2O3. This opens for unique nanotechnological applications and novel fundamental studies of actomyosin motor function. Here, we provide a comparison of myosin-driven actin filament motility on Al2O3 to both nitrocellulose and trimethylchlorosilane derivatized surfaces. We also show that actomyosin motility on the less than 200 nm wide tips of arrays of Al2O3-coated nanowires can be used to control the number, and density, of myosin-actin attachment points. Results obtained using nanowire arrays with different inter-wire spacing are consistent with the idea that the actin filament sliding velocity is determined both by the total number and the average density of attached myosin heads along the actin filament. Further, the results are consistent with buckling of long myosin-free segments of the filaments as a factor underlying reduced velocity. On the other hand, the findings do not support a mechanistic role in decreasing velocity, of increased nearest neighbor distance between available myosin heads. Our results open up for more advanced studies that may use nanowire-based structures for fundamental investigations of molecular motors, including the possibility to create a nanowire-templated bottom-up assembly of 3D, muscle-like structures.

Keywords
Actin, aluminum oxide, in vitro motility assay, myosin, oxide-coated nanowire, sarcomere
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46290 (URN)10.1109/TNB.2015.2412036 (DOI)000355321100005 ()25823040 (PubMedID)2-s2.0-84930670434 (Scopus ID)
Available from: 2015-09-14 Created: 2015-09-14 Last updated: 2017-12-04Bibliographically approved
ten Siethoff, L., Lard, M., Generosi, J., Andersson, H. S., Linke, H. & Månsson, A. (2014). Molecular Motor Propelled Filaments Reveal Light-Guiding in Nanowire Arrays for Enhanced Biosensing. Nano letters (Print), 14(2), 737-742
Open this publication in new window or tab >>Molecular Motor Propelled Filaments Reveal Light-Guiding in Nanowire Arrays for Enhanced Biosensing
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2014 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 14, no 2, p. 737-742Article in journal (Refereed) Published
Abstract [en]

Semiconductor nanowire arrays offer significant potential for biosensing applications with optical read-out due to their high surface area and due to the unique optical properties of one-dimensional materials. A challenge for optical read-out of analyte-binding to the nanowires is the need to efficiently collect and detect light from a three-dimensional volume. Here we show that light from fluorophores attached along Several mu m long vertical Al2O3 coated gallium phosphide nanowires couples into the wires, is guided along them and emitted at the tip. This enables effective collection of light emitted by fluorescent analytes located at different focal planes along the nanowire. We unequivocally demonstrate the light-guiding effect using a novel method whereby the changes in emitted fluorescence intensity are observed when fluorescent cytoskeletal filaments are propelled by molecular motors along the wires. The findings are discussed in relation to nanobiosensor developments, other nanotechnological applications, and fundamental studies of motor function.

Keywords
Nanobiotechnology, in vitro motility assay, photonics, actin, myosin, actomyosin
National Category
Chemical Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-32784 (URN)10.1021/nl404032k (DOI)000331343900052 ()2-s2.0-84894188778 (Scopus ID)
Funder
EU, European Research Council, 228971Swedish Research Council, 621-2010-5146, 621-2010-4527
Available from: 2014-03-17 Created: 2014-03-14 Last updated: 2018-11-02Bibliographically approved
Lard, M., ten Siethoff, L., Generosi, J., Månsson, A. & Linke, H. (2014). Molecular Motor Transport through Hollow Nanowires. Nano letters (Print), 14(6), 3041-3046
Open this publication in new window or tab >>Molecular Motor Transport through Hollow Nanowires
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2014 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 14, no 6, p. 3041-3046Article in journal (Refereed) Published
Abstract [en]

Biomolecular motors offer self-propelled, directed transport in designed microscale networks and can potentially replace pump-driven nanofluidics. However, in existing systems, transportation is limited to the two-dimensional plane. Here we demonstrate fully one-dimensional (1D) myosin-driven motion of fluorescent probes (actin filaments) through 80 nm wide, Al2O3 hollow nanowires of micrometer length. The motor-driven transport is orders of magnitude faster than would be possible by passive diffusion. The system represents a necessary element for advanced devices based on gliding assays, for example, in lab-on-a-chip systems with channel crossings and in pumpless nanosyringes. It may also serve as a scaffold for bottom-up assembly of muscle proteins into actin ordered contractile units, mimicking the muscle sarcomere.

Keywords
Hollow nanowires, actin, myosin, molecular motors, motor proteins, 1D gliding assay
National Category
Materials Chemistry
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-36091 (URN)10.1021/nl404714b (DOI)000337337100012 ()2-s2.0-84902250949 (Scopus ID)
Available from: 2014-07-17 Created: 2014-07-17 Last updated: 2017-12-05Bibliographically approved
Persson, M., Bengtsson, E., ten Siethoff, L. & Månsson, A. (2014). Non-Linear Cross-Bridge Elasticity, ATP-Independent Detachment and ATP-Velocity Relationships for Skeletal Muscle Actomyosin. Paper presented at 58th Annual Meeting of the Biophysical-Society, FEB 15-19, 2014, San Francisco, CA. Biophysical Journal, 106(2), 158A-158A
Open this publication in new window or tab >>Non-Linear Cross-Bridge Elasticity, ATP-Independent Detachment and ATP-Velocity Relationships for Skeletal Muscle Actomyosin
2014 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 106, no 2, p. 158A-158AArticle in journal, Meeting abstract (Other academic) Published
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-36106 (URN)000337000400783 ()
Conference
58th Annual Meeting of the Biophysical-Society, FEB 15-19, 2014, San Francisco, CA
Available from: 2014-07-17 Created: 2014-07-17 Last updated: 2017-12-05Bibliographically approved
Månsson, A., ten Siethoff, L., Lard, M., Generosi, J., Andersson, H. S. & Linke, H. (2014). Three-Dimensionally Constrained Actomyosin Motility on Oxide Coated Semiconductor Nanowires. Paper presented at 58th Annual Meeting of the Biophysical-Society, FEB 15-19, 2014, San Francisco, CA. Biophysical Journal, 106(2), 453A-453A
Open this publication in new window or tab >>Three-Dimensionally Constrained Actomyosin Motility on Oxide Coated Semiconductor Nanowires
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2014 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 106, no 2, p. 453A-453AArticle in journal, Meeting abstract (Other academic) Published
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-36107 (URN)000337000402525 ()
Conference
58th Annual Meeting of the Biophysical-Society, FEB 15-19, 2014, San Francisco, CA
Available from: 2014-07-17 Created: 2014-07-17 Last updated: 2017-12-05Bibliographically approved
Kumar, S., ten Siethoff, L., Persson, M., Albet-Torres, N. & Månsson, A. (2013). Magnetic capture from blood rescues molecular motor function in diagnostic nanodevices. Journal of Nanobiotechnology, 11, Article ID 14.
Open this publication in new window or tab >>Magnetic capture from blood rescues molecular motor function in diagnostic nanodevices
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2013 (English)In: Journal of Nanobiotechnology, ISSN 1477-3155, E-ISSN 1477-3155, Vol. 11, article id 14Article in journal (Refereed) Published
Abstract [en]

Background: Introduction of effective point-of-care devices for use in medical diagnostics is part of strategies to combat accelerating health-care costs. Molecular motor driven nanodevices have unique potentials in this regard due to unprecedented level of miniaturization and independence of external pumps. However motor function has been found to be inhibited by body fluids. Results: We report here that a unique procedure, combining separation steps that rely on antibody-antigen interactions, magnetic forces applied to magnetic nanoparticles (MPs) and the specificity of the actomyosin bond, can circumvent the deleterious effects of body fluids (e.g. blood serum). The procedure encompasses the following steps: (i) capture of analyte molecules from serum by MP-antibody conjugates, (ii) pelleting of MP-antibody-analyte complexes, using a magnetic field, followed by exchange of serum for optimized biological buffer, (iii) mixing of MP-antibody-analyte complexes with actin filaments conjugated with same polyclonal antibodies as the magnetic nanoparticles. This causes complex formation: MP-antibody-analyte-antibody-actin, and magnetic separation is used to enrich the complexes. Finally (iv) the complexes are introduced into a nanodevice for specific binding via actin filaments to surface adsorbed molecular motors (heavy meromyosin). The number of actin filaments bound to the motors in the latter step was significantly increased above the control value if protein analyte (50-60 nM) was present in serum (in step i) suggesting appreciable formation and enrichment of the MP-antibody-analyte-antibody-actin complexes. Furthermore, addition of ATP demonstrated maintained heavy meromyosin driven propulsion of actin filaments showing that the serum induced inhibition was alleviated. Detailed analysis of the procedure i-iv, using fluorescence microscopy and spectroscopy identified main targets for future optimization. Conclusion: The results demonstrate a promising approach for capturing analytes from serum for subsequent motor driven separation/detection. Indeed, the observed increase in actin filament number, in itself, signals the presence of analyte at clinically relevant nM concentration without the need for further motor driven concentration. Our analysis suggests that exchange of polyclonal for monoclonal antibodies would be a critical improvement, opening for a first clinically useful molecular motor driven lab-on-a-chip device.

Keywords
Magnetic nanoparticle, Biomolecular motor, Myosin, Nanoseparation, Lab-on-a-chip, Bioconjugation
National Category
Medical Biotechnology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-27565 (URN)10.1186/1477-3155-11-14 (DOI)000319316500001 ()2-s2.0-84877003874 (Scopus ID)
Available from: 2013-07-17 Created: 2013-07-17 Last updated: 2017-12-06Bibliographically approved
Lard, M., ten Siethoff, L., Generosi, J., Andersson, H. S., Månsson, A. & Linke, H. (2013). Nanowire Interfacing with Molecular Motors: Light Guiding and Tunneling. In: : . Paper presented at Annual Symposium of the Nanometer Structure Consortium. Opportunities in Novel Nanocharacterization Methods, Oct 9th, 2013, Lund, Sweden. Lund
Open this publication in new window or tab >>Nanowire Interfacing with Molecular Motors: Light Guiding and Tunneling
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2013 (English)Conference paper, Poster (with or without abstract) (Other academic)
Place, publisher, year, edition, pages
Lund: , 2013
Keywords
in vitro motility assay, actin, myosin
National Category
Materials Chemistry
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46509 (URN)
Conference
Annual Symposium of the Nanometer Structure Consortium. Opportunities in Novel Nanocharacterization Methods, Oct 9th, 2013, Lund, Sweden
Note

Ej belagd 20151104

Available from: 2015-09-28 Created: 2015-09-28 Last updated: 2018-11-02Bibliographically approved
Persson, M., Bengtsson, E., ten Siethoff, L. & Månsson, A. (2013). Nonlinear Cross-Bridge Elasticity and Post-Power-Stroke Events in Fast Skeletal Muscle Actomyosin. Biophysical Journal, 105(8), 1871-1881
Open this publication in new window or tab >>Nonlinear Cross-Bridge Elasticity and Post-Power-Stroke Events in Fast Skeletal Muscle Actomyosin
2013 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 105, no 8, p. 1871-1881Article in journal (Refereed) Published
Abstract [en]

Generation-of force and movement by actomyosin cross-bridges is the molecular basis of muscle contraction, but generally accepted ideas about cross-bridge properties have recently been questioned. Of the utmost significance, evidence for nonlinear cross-bridge elasticity has been presented. We here investigate how this and other newly discovered or postulated phenomena would modify cross-bridge operation, with focus on post-power-stroke events. First, as an experimental basis, we present evidence for a hyperbolic [MgATP]-velocity relationship of heavy-meromyosin-propelled actin filaments in the in vitro motility assay using fast rabbit skeletal muscle myosin (28-29 degrees C). As the hyperbolic [MgATP]-velocity relationship was not consistent with interhead cooperativity, we developed a cross-bridge model with independent myosin heads and strain-dependent interstate transition rates. The model, implemented with inclusion of MgATP-independent detachment from the rigor state, as suggested by previous single-molecule mechanics experiments, accounts well for the [MgATP]-velocity relationship if nonlinear cross-bridge elasticity is assumed, but not if linear cross-bridge elasticity is assumed. In addition, a better fit is obtained with load-independent than with load-dependent MgATP-induced detachment rate. We discuss our results in relation to previous data showing a nonhyperbolic [MgATP1-velocity relationship when actin filaments are propelled by myosin subfragment 1 or full-length myosin. We also consider the implications of our results for characterization of the cross-bridge elasticity in the filament lattice of muscle.

National Category
Biophysics
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-30999 (URN)10.1016/j.bpj.2013.08.044 (DOI)000325838500017 ()2-s2.0-84886005960 (Scopus ID)
Available from: 2013-12-06 Created: 2013-12-06 Last updated: 2017-12-06Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-6878-3142

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