lnu.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Publications (9 of 9) Show all publications
Andersson, H. S., Jacobsson, E., Rosengren, K. J., Strand, M. & Göransson, U. (2016). Discovery of novel ion-channel active peptide toxins in a North Sea Ribbon Worm. In: : . Paper presented at Analysdagarna 2016, Umeå, 14-17 juni 2016. Svenska kemistsamfundet..
Open this publication in new window or tab >>Discovery of novel ion-channel active peptide toxins in a North Sea Ribbon Worm
Show others...
2016 (English)Conference paper, Oral presentation with published abstract (Other academic)
Abstract [en]

Ribbon worms (nemerteans) are marine predators, which capture their prey using a proboscis containing a mixture of toxins which brings on rapid paralysis [1]. In addition, their epidermis contains thick mucus of similar toxic constitution. One very potent toxin reported in ribbon worm mucus is tetrodotoxin (TTX). However, despite significant efforts, Strand et al. [2] were unable to detect any TTX, neither in the mucus of the ribbon worm Lineus longissimus, nor from Vibrio alginolyticus cultures isolated from and cultivated in the mucus. These observations challenged the notion of general presence of TTX in ribbon worm mucus, and prompted us to look for other toxins [3]. Using LC-MS analysis of mucus extracts, we identified three peptides present in significant amounts. The peptides were sequenced using a combination of MS/MS analysis and transcriptomics, and whereas one of them strongly resembles the only peptide toxin previously characterized from ribbon worms, Neurotoxin B-IV [4], the other two were found to represent a previously unknown class of peptide toxins. The most abundant of these was synthesized, and its 3D structure determined. Preliminary toxicity tests on shore crab (C. maenas) indicated toxicity (through paralysis) on par with that of TTX. Further analyses have indicated that its toxic effects are due to binding to voltage sensitive sodium channels.

 

With L. longissimus as our primary target, we are now mapping the presence of peptide toxins in ribbon worms, with the objectives to establish routes for synthesis, and to characterize the biological activities and structures of these peptides. The number of peptides of this novel class is increasing, and synthesis and characterization is well underway. The striking potencies of these peptides make them potentially amenable as novel insecticidal or anthelmintic leads, pharmacological tools or in biotechnology applications.

 

References

1. Strand M, Sundberg P. Nationalnyckeln till Sveriges flora och fauna [DO-DP]. Stjärnmaskar-Slemmaskar: Sipuncula-Nemertea: Artdatabanken, SLU; 2010.

2. Strand M, Hedstrom M, Seth H, McEvoy EG, Jacobsson E, Goransson U, Andersson HS, Sundberg P. The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus-Just a False Positive? Marine Drugs. 2016;14(4).

3. Strand M, Andersson HS. Slemmaskens hemlighet. Forskning & Framsteg. 2016;(2):26-33.

4. Blumenthal KM, Kem WR. Structure and action of heteronemertine polypeptide toxins. Primary structure of Cerebratulus lacteus toxin B-IV. The Journal of Biological Chemistry. 1976;251(19):6025-9.

Keywords
nemertea, peptide, ion-channel
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-54031 (URN)
Conference
Analysdagarna 2016, Umeå, 14-17 juni 2016. Svenska kemistsamfundet.
Funder
Swedish Research Council
Available from: 2016-06-20 Created: 2016-06-20 Last updated: 2020-05-13Bibliographically approved
Jacobsson, E., Andersson, H. S., Strand, M., Lebbe, E., Eriksson, C., Peigneur, S., . . . Göransson, U. (2016). Peptide toxins from the longest animal on earth.. Paper presented at Joint Natural Products Conference, July 25-27, Copenhagen, Denmark. Planta Medica, 82(Supplement 1), Article ID YRW3.
Open this publication in new window or tab >>Peptide toxins from the longest animal on earth.
Show others...
2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82, no Supplement 1, article id YRW3Article in journal, Meeting abstract (Other academic) Published
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Medical Chemistry
Identifiers
urn:nbn:se:lnu:diva-56305 (URN)10.1055/s-0036-1596160 (DOI)000411789300986 ()
Conference
Joint Natural Products Conference, July 25-27, Copenhagen, Denmark
Funder
Swedish Research Council
Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2020-05-13Bibliographically approved
Strand, M. & Andersson, H. S. (2016). Slemmaskens hemlighet. Forskning & Framsteg (2), 26-33
Open this publication in new window or tab >>Slemmaskens hemlighet
2016 (Swedish)In: Forskning & Framsteg, ISSN 0015-7937, no 2, p. 26-33Article in journal (Other (popular science, discussion, etc.)) Published
Abstract [sv]

Nyckeln till framtidens mediciner kan gömma sig hos slemmiga, giftiga maskar som lever på havets botten. Här berättar marinbiologen Malin Strand och biokemisten Håkan Andersson om jakten som ledde till en oväntad upptäckt – och som tog slemmasken från det marinbiologiska laboratoriet på Tjärnö och Sveriges västkust till kemilaboratoriet i Uppsala.

Place, publisher, year, edition, pages
Stockholm: Stiftelsen Forskning & Framsteg, 2016
Keywords
nemertiner, tetrodotoxin, marinbiologi, slemmaskar
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-49658 (URN)
Funder
Swedish Research Council
Available from: 2016-02-08 Created: 2016-02-08 Last updated: 2020-01-29Bibliographically approved
Strand, M., Hedström, M., Seth, H., McEvoy, E. G., Jacobsson, E., Göransson, U., . . . Sundberg, P. (2016). The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus: Just a False Positive?. Marine Drugs, 14(4), Article ID 63.
Open this publication in new window or tab >>The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus: Just a False Positive?
Show others...
2016 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 14, no 4, article id 63Article in journal (Refereed) Published
Abstract [en]

We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel–dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound <5 kDa, and via a different mechanism of action than that of TTX. The similarity in mass between TTX and the Vibrio-produced low-molecular-weight, non-toxic compound invokes that thorough analysis is required when assessing TTX production. Based on our findings, we suggest that re-examination of some published claims of TTX production may be warranted.

Place, publisher, year, edition, pages
Basel: MDPI, 2016
Keywords
LC-MS; mucus; axonal conductance; Vibrio; nemertean; tetrodotoxin, slemmaskar, nemertiner
National Category
Analytical Chemistry
Research subject
Chemistry, Medical Chemistry
Identifiers
urn:nbn:se:lnu:diva-51493 (URN)10.3390/md14040063 (DOI)000374588000002 ()2-s2.0-84962751120 (Scopus ID)
Available from: 2016-03-29 Created: 2016-03-29 Last updated: 2020-01-28Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Hedström, M., Seth, H., Sundberg, P., . . . Göransson, U. (2016). The toxicity of ribbon worms: alpha-nemertides or tetrodotoxin, or both?. Paper presented at Joint Natural Products Conference, July 25-27, Copenhagen, Denmark. Planta Medica, 82(Supplement 1), Article ID P549.
Open this publication in new window or tab >>The toxicity of ribbon worms: alpha-nemertides or tetrodotoxin, or both?
Show others...
2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82, no Supplement 1, article id P549Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

The marine ribbon worms (nemerteans) are predators which capture their prey by everting a proboscis carrying a mixture of toxins which brings on rapid paralysis [1]. Moreover, ribbon worms have a thick layer of epidermal mucus of similar constitution. Tetrodotoxin (TTX) has been identified as one of these toxins [2]. The extreme toxicity of TTX (lethal by ingestion of 0.5-2 mg) is due to its ability to block voltage-gated sodium channels. Although several bacterial species (among these Vibrio sp.) have been linked to its synthesis, the biogenic origin and biosynthesis is unclear. One hypothesis is that TTX production occurs in a symbiotic relationship with its host, in this case the ribbon worm [3]. We have made significant effort to identify TTX in a setup for production through the cultivation of Vibrio alginolyticus in nutrient broth infused with mucus from the ribbon worm Lineus longissimus. Toxicity was demonstrated by fraction injections into shore crabs, but no TTX was found, and it could be shown conclusively that toxicity was unrelated to TTX and the Vibrio culture itself, and rather a constituent of the ribbon worm mucus [4]. The following studies led us to the discovery of a new class of peptides, the alpha-nemertides, in the mucus of the ribbon worms, which could be directly linked to the toxic effects. A literature review of the available evidence for TTX in ribbon worms show that the evidence in most cases are indirect, although notable exceptions exist. This points to the necessity to further investigate the presence and roles of TTX and alpha-nemertides in ribbon worms.

National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Medical Chemistry
Identifiers
urn:nbn:se:lnu:diva-56306 (URN)10.1055/s-0036-1596617 (DOI)000411789300441 ()
Conference
Joint Natural Products Conference, July 25-27, Copenhagen, Denmark
Funder
Swedish Research CouncilThe Crafoord Foundation
Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2020-05-13Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Hedström, M., Seth, H., McEvoy, E. G., . . . Göransson, U. (2015). Discovery of peptide toxins in ribbon worms: challenging claims of tetrodotoxin production. In: : . Paper presented at International Society of Chemical Ecology Meeting, June 29-July 3, Stockholm (pp. Poster 81).
Open this publication in new window or tab >>Discovery of peptide toxins in ribbon worms: challenging claims of tetrodotoxin production
Show others...
2015 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
nemertea, peptide toxin, tetrodotoxin
National Category
Other Chemistry Topics Analytical Chemistry
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46479 (URN)
Conference
International Society of Chemical Ecology Meeting, June 29-July 3, Stockholm
Funder
Swedish Research Council
Available from: 2015-09-25 Created: 2015-09-25 Last updated: 2020-01-28Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Rosengren, K. J., Andrén, P., Strand, M. & Göransson, U. (2015). Discovery of peptide toxins in the bootlace worm, the world's longest animal. In: : . Paper presented at 9th European Conference on Marine Natural Products, Aug 30-Sep 2, Glasgow, Scotland (pp. P1:8).
Open this publication in new window or tab >>Discovery of peptide toxins in the bootlace worm, the world's longest animal
Show others...
2015 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
pharmacognosy, peptide toxin, nemertea
National Category
Other Chemistry Topics
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46474 (URN)
Conference
9th European Conference on Marine Natural Products, Aug 30-Sep 2, Glasgow, Scotland
Funder
Swedish Research Council
Available from: 2015-09-25 Created: 2015-09-25 Last updated: 2020-01-28Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Hedström, M., Seth, H., McEvoy, E. G., . . . Strand, M. (2015). Discovery of peptide toxins in the world’s longest animal (The bootlace worm; Lineus longissimus): challenging claims of tetrodotoxin production.. In: : . Paper presented at Nordic Natural Products Conference – “Natural Products – Past, Present & Future”, June 15th-16th, Visby, Sweden (pp. L08).
Open this publication in new window or tab >>Discovery of peptide toxins in the world’s longest animal (The bootlace worm; Lineus longissimus): challenging claims of tetrodotoxin production.
Show others...
2015 (English)Conference paper, Oral presentation with published abstract (Other academic)
Keywords
nemertea, tetrodotoxin, peptide toxin, pharmacognosy
National Category
Medicinal Chemistry
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46481 (URN)
Conference
Nordic Natural Products Conference – “Natural Products – Past, Present & Future”, June 15th-16th, Visby, Sweden
Funder
Swedish Research Council
Available from: 2015-09-25 Created: 2015-09-25 Last updated: 2018-11-02Bibliographically approved
Jacobsson, E., Andersson, H. S., Strand, M., Eriksson, C. & Göransson, U. (2015). Peptide toxins from L. longissimus: extraction, biological activity, structure and production. In: : . Paper presented at Nordic Natural Products Conference - "Natural Products - Past, Present & Future, June 15-16, Visby, Sweden (pp. L05).
Open this publication in new window or tab >>Peptide toxins from L. longissimus: extraction, biological activity, structure and production
Show others...
2015 (English)Conference paper, Oral presentation with published abstract (Other academic)
Keywords
nemertea, peptide toxin, pharmacognosy
National Category
Medicinal Chemistry
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46480 (URN)
Conference
Nordic Natural Products Conference - "Natural Products - Past, Present & Future, June 15-16, Visby, Sweden
Funder
Swedish Research Council
Available from: 2015-09-25 Created: 2015-09-25 Last updated: 2020-01-28Bibliographically approved
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-8144-9592

Search in DiVA

Show all publications