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Publications (7 of 7) Show all publications
Göransson, U., Gunasekera, S., Malik, S., Park, S., Slazak, B., Jacobsson, E., . . . Strömstedt, A. (2016). Peptide biodiscovery from plants and animals: structure to function. Paper presented at Joint Natural Products Conference, July 25-27, Copenhagen, Denmark. Planta Medica, 82(Supplement 1), Article ID SL49.
Open this publication in new window or tab >>Peptide biodiscovery from plants and animals: structure to function
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82, no Supplement 1, article id SL49Article in journal, Meeting abstract (Other academic) Published
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Medical Chemistry
Identifiers
urn:nbn:se:lnu:diva-56307 (URN)10.1055/s-0036-1596156 (DOI)000411789300953 ()
Conference
Joint Natural Products Conference, July 25-27, Copenhagen, Denmark
Funder
Swedish Research Council
Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2018-11-02Bibliographically approved
Jacobsson, E., Andersson, H. S., Strand, M., Lebbe, E., Eriksson, C., Peigneur, S., . . . Göransson, U. (2016). Peptide toxins from the longest animal on earth.. Paper presented at Joint Natural Products Conference, July 25-27, Copenhagen, Denmark. Planta Medica, 82(Supplement 1), Article ID YRW3.
Open this publication in new window or tab >>Peptide toxins from the longest animal on earth.
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82, no Supplement 1, article id YRW3Article in journal, Meeting abstract (Other academic) Published
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Medical Chemistry
Identifiers
urn:nbn:se:lnu:diva-56305 (URN)10.1055/s-0036-1596160 (DOI)000411789300986 ()
Conference
Joint Natural Products Conference, July 25-27, Copenhagen, Denmark
Funder
Swedish Research Council
Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2018-11-02Bibliographically approved
Strand, M., Hedström, M., Seth, H., McEvoy, E. G., Jacobsson, E., Göransson, U., . . . Sundberg, P. (2016). The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus: Just a False Positive?. Marine Drugs, 14(4), Article ID 63.
Open this publication in new window or tab >>The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus: Just a False Positive?
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2016 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 14, no 4, article id 63Article in journal (Refereed) Published
Abstract [en]

We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel–dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound <5 kDa, and via a different mechanism of action than that of TTX. The similarity in mass between TTX and the Vibrio-produced low-molecular-weight, non-toxic compound invokes that thorough analysis is required when assessing TTX production. Based on our findings, we suggest that re-examination of some published claims of TTX production may be warranted.

Place, publisher, year, edition, pages
Basel: MDPI, 2016
Keywords
LC-MS; mucus; axonal conductance; Vibrio; nemertean; tetrodotoxin, slemmaskar, nemertiner
National Category
Analytical Chemistry
Research subject
Chemistry, Medical Chemistry
Identifiers
urn:nbn:se:lnu:diva-51493 (URN)10.3390/md14040063 (DOI)000374588000002 ()2-s2.0-84962751120 (Scopus ID)
Available from: 2016-03-29 Created: 2016-03-29 Last updated: 2018-11-02Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Hedström, M., Seth, H., Sundberg, P., . . . Göransson, U. (2016). The toxicity of ribbon worms: alpha-nemertides or tetrodotoxin, or both?. Paper presented at Joint Natural Products Conference, July 25-27, Copenhagen, Denmark. Planta Medica, 82(Supplement 1), Article ID P549.
Open this publication in new window or tab >>The toxicity of ribbon worms: alpha-nemertides or tetrodotoxin, or both?
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2016 (English)In: Planta Medica, ISSN 0032-0943, E-ISSN 1439-0221, Vol. 82, no Supplement 1, article id P549Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

The marine ribbon worms (nemerteans) are predators which capture their prey by everting a proboscis carrying a mixture of toxins which brings on rapid paralysis [1]. Moreover, ribbon worms have a thick layer of epidermal mucus of similar constitution. Tetrodotoxin (TTX) has been identified as one of these toxins [2]. The extreme toxicity of TTX (lethal by ingestion of 0.5-2 mg) is due to its ability to block voltage-gated sodium channels. Although several bacterial species (among these Vibrio sp.) have been linked to its synthesis, the biogenic origin and biosynthesis is unclear. One hypothesis is that TTX production occurs in a symbiotic relationship with its host, in this case the ribbon worm [3]. We have made significant effort to identify TTX in a setup for production through the cultivation of Vibrio alginolyticus in nutrient broth infused with mucus from the ribbon worm Lineus longissimus. Toxicity was demonstrated by fraction injections into shore crabs, but no TTX was found, and it could be shown conclusively that toxicity was unrelated to TTX and the Vibrio culture itself, and rather a constituent of the ribbon worm mucus [4]. The following studies led us to the discovery of a new class of peptides, the alpha-nemertides, in the mucus of the ribbon worms, which could be directly linked to the toxic effects. A literature review of the available evidence for TTX in ribbon worms show that the evidence in most cases are indirect, although notable exceptions exist. This points to the necessity to further investigate the presence and roles of TTX and alpha-nemertides in ribbon worms.

National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Medical Chemistry
Identifiers
urn:nbn:se:lnu:diva-56306 (URN)10.1055/s-0036-1596617 (DOI)000411789300441 ()
Conference
Joint Natural Products Conference, July 25-27, Copenhagen, Denmark
Funder
Swedish Research CouncilThe Crafoord Foundation
Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2018-11-02Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Hedström, M., Seth, H., McEvoy, E. G., . . . Göransson, U. (2015). Discovery of peptide toxins in ribbon worms: challenging claims of tetrodotoxin production. In: : . Paper presented at International Society of Chemical Ecology Meeting, June 29-July 3, Stockholm (pp. Poster 81).
Open this publication in new window or tab >>Discovery of peptide toxins in ribbon worms: challenging claims of tetrodotoxin production
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2015 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
nemertea, peptide toxin, tetrodotoxin
National Category
Other Chemistry Topics Analytical Chemistry
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46479 (URN)
Conference
International Society of Chemical Ecology Meeting, June 29-July 3, Stockholm
Funder
Swedish Research Council
Available from: 2015-09-25 Created: 2015-09-25 Last updated: 2018-11-02Bibliographically approved
Andersson, H. S., Jacobsson, E., Eriksson, C., Rosengren, K. J., Andrén, P., Strand, M. & Göransson, U. (2015). Discovery of peptide toxins in the bootlace worm, the world's longest animal. In: : . Paper presented at 9th European Conference on Marine Natural Products, Aug 30-Sep 2, Glasgow, Scotland (pp. P1:8).
Open this publication in new window or tab >>Discovery of peptide toxins in the bootlace worm, the world's longest animal
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2015 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
pharmacognosy, peptide toxin, nemertea
National Category
Other Chemistry Topics
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46474 (URN)
Conference
9th European Conference on Marine Natural Products, Aug 30-Sep 2, Glasgow, Scotland
Funder
Swedish Research Council
Available from: 2015-09-25 Created: 2015-09-25 Last updated: 2018-11-02Bibliographically approved
Jacobsson, E., Andersson, H. S., Strand, M., Eriksson, C. & Göransson, U. (2015). Peptide toxins from L. longissimus: extraction, biological activity, structure and production. In: : . Paper presented at Nordic Natural Products Conference - "Natural Products - Past, Present & Future, June 15-16, Visby, Sweden (pp. L05).
Open this publication in new window or tab >>Peptide toxins from L. longissimus: extraction, biological activity, structure and production
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2015 (English)Conference paper, Oral presentation with published abstract (Other academic)
Keywords
nemertea, peptide toxin, pharmacognosy
National Category
Medicinal Chemistry
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-46480 (URN)
Conference
Nordic Natural Products Conference - "Natural Products - Past, Present & Future, June 15-16, Visby, Sweden
Funder
Swedish Research Council
Available from: 2015-09-25 Created: 2015-09-25 Last updated: 2018-11-02Bibliographically approved
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-5005-9612

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