lnu.sePublications
Change search
Link to record
Permanent link

Direct link
Alternative names
Publications (10 of 99) Show all publications
Guo, M., Chen, L., Fang, T., Wang, R., Nuraje, N. & Brodelius, P. E. (2021). Synthesis, properties and applications of self-repairing carbohydrates as smart materials via thermally reversible DA bonds. Polymers for Advanced Technologies, 32(3), 1026-1037
Open this publication in new window or tab >>Synthesis, properties and applications of self-repairing carbohydrates as smart materials via thermally reversible DA bonds
Show others...
2021 (English)In: Polymers for Advanced Technologies, ISSN 1042-7147, E-ISSN 1099-1581, Vol. 32, no 3, p. 1026-1037Article in journal (Refereed) Published
Abstract [en]

Self-healing carbohydrate polymers were synthesized by Diels-Alder reaction. Intermediate products and the carbohydrate matrices were characterized by Fourier transform infrared spectroscopy (FT-IR) and H-1 NMR, while the thermally reversible properties were assessed by FT-IR and differential scanning calorimetry. The mechanical properties, water absorption, and enzymatic degradation of starch/PVA/modified carbohydrate films were examined, as well as the relationship of the properties to the DA and rDA reactions. These results showed that DA bonds were introduced into the carbohydrate polymers successfully and endow the material with self-healing thermal recyclability. The mixed films exhibited alternating strong and weak mechanical properties upon cycling through the DA and rDA reactions. Water absorption was limited and the films demonstrated good water resistance. The status of the DA bonds was found not to affect the enzymatic degradation rates of the various carbohydrate films.

Place, publisher, year, edition, pages
John Wiley & Sons, 2021
Keywords
biodegradable film, carbohydrates, Diels&#8208, Alder reaction, self&#8208, healing
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-99612 (URN)10.1002/pat.5150 (DOI)000583760700001 ()2-s2.0-85096793505 (Scopus ID)2020 (Local ID)2020 (Archive number)2020 (OAI)
Available from: 2021-01-12 Created: 2021-01-12 Last updated: 2021-04-14Bibliographically approved
Czechowski, T., Weathers, P. J., Brodelius, P. E., Brown, G. D. & Graham, I. A. (2020). Editorial: Artemisinin-From Traditional Chinese Medicine to Artemisinin Combination Therapies; Four Decades of Research on the Biochemistry, Physiology, and Breeding of Artemisia annua. Frontiers in Plant Science, 11, 1-3, Article ID 594565.
Open this publication in new window or tab >>Editorial: Artemisinin-From Traditional Chinese Medicine to Artemisinin Combination Therapies; Four Decades of Research on the Biochemistry, Physiology, and Breeding of Artemisia annua
Show others...
2020 (English)In: Frontiers in Plant Science, E-ISSN 1664-462X, Vol. 11, p. 1-3, article id 594565Article in journal, Editorial material (Other academic) Published
Place, publisher, year, edition, pages
Frontiers Media S.A., 2020
Keywords
Artemisia annua, artemisinin, semi-synthetics, molecular breeding, malaria
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-98625 (URN)10.3389/fpls.2020.594565 (DOI)000576431400001 ()33042197 (PubMedID)2-s2.0-85091921333 (Scopus ID)
Available from: 2020-10-23 Created: 2020-10-23 Last updated: 2024-01-17Bibliographically approved
Guo, M., Zhao, X., Brodelius, P. E., Fang, L., Sun, Z. & Wang, R. (2020). The Difference of Serum Protein Transport between Echinosides and Verbascoside. Journal of the Chemical Society of Pakistan, 42(3), 369-382
Open this publication in new window or tab >>The Difference of Serum Protein Transport between Echinosides and Verbascoside
Show others...
2020 (English)In: Journal of the Chemical Society of Pakistan, ISSN 0253-5106, Vol. 42, no 3, p. 369-382Article in journal (Refereed) Published
Abstract [en]

Verbascoside (VER) is the enzymatic hydrolysis product of echinacoside (ECH). The molecular structures of ECH and VER have different glucosyl groups so they bind to serum albumin in different ways, resulting in different pharmacological actions. In this report, we have examined the binding characteristics between human serum albumin (HSA) and ECH/VER by molecular modeling and spectroscopic approaches. Molecular modeling revealed that VER bound to HSA mainly through hydrogen bonds, van der Waals forces and hydrophobic forces. The spectroscopic results showed that the interactions between HSA and VER/ECH involved a static binding process, and the bonding strength of the VER-HSA complex was stronger than that of the ECH-HSA complex. The value of the binding distances (r) was low, which indicated the occurrence of energy transfer. The reaction conformational pattern of HSA-VER and HSA-ECH gave a "two-state model" based on fluorescent phase diagram analysis. According to the thermodynamic model, the main forces between interaction of VER and HSA were hydrogen bonds and van der Waals forces, whereas the interaction between ECH and HSA was hydrophobic force. The fluorescence polarization analysis demonstrated that the interaction between HSA and VER or ECH generated a non-covalent complex. Compared with ECH, VER was more likely to bind with HSA because of its smaller molecular size and low polarity. The results of the spectral analysis concurred with the molecular modeling data, which provides a helpful reference for the study of the molecular reaction mechanism of VER/ECH binding to HSA.

Place, publisher, year, edition, pages
The chemical Society of Pakistan, 2020
Keywords
Verbascoside, Echinacosid, Serum, Albumin, Spectrum, Experiment, Molecular modeling
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-97728 (URN)000549342800004 ()2-s2.0-85097898817 (Scopus ID)
Available from: 2020-08-27 Created: 2020-08-27 Last updated: 2021-05-11Bibliographically approved
Muthusamy, S. D., Vetukuri, R. R., Lundgren, A., Ganji, S., Zhu, L.-H., Brodelius, P. E. & Kanagarajan, S. (2020). Transient expression and purification of β-caryophyllene synthase in Nicotiana benthamiana to produce β-caryophyllene in vitro. PeerJ, 8, 1-21, Article ID e8904.
Open this publication in new window or tab >>Transient expression and purification of β-caryophyllene synthase in Nicotiana benthamiana to produce β-caryophyllene in vitro
Show others...
2020 (English)In: PeerJ, E-ISSN 2167-8359, Vol. 8, p. 1-21, article id e8904Article in journal (Refereed) Published
Abstract [en]

The sesquiterpene beta-caryophyllene is an ubiquitous component in many plants that has commercially been used as an aroma in cosmetics and perfumes. Recent studies have shown its potential use as a therapeutic agent and biofuel. Currently, beta-caryophyllene is isolated from large amounts of plant material. Molecular farming based on the Nicotiana benthamiana transient expression system may be used for a more sustainable production of beta-caryophyllene. In this study, a full-length cDNA of a new duplicated beta-caryophyllene synthase from Artemisia annua (AaCPS1) was isolated and functionally characterized. In order to produce beta-caryophyllene in vitro, the AaCPS1 was cloned into a plant viral-based vector pEAQ-HT. Subsequently, the plasmid was transferred into the Agrobacterium and agroinfiltrated into N. benthamiana leaves. The AaCPS1 expression was analyzed by quantitative PCR at different time points after agroinfiltration. The highest level of transcripts was observed at 9 days post infiltration (dpi). The AaCPS1 protein was extracted from the leaves at 9 dpi and purified by cobalt-nitrilotriacetate (Co-NTA) affinity chromatography using histidine tag with a yield of 89 mg kg(-1). fresh weight of leaves. The protein expression of AaCPS1 was also confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analyses. AaCPS1 protein uses farnesyl diphosphate (FPP) as a substrate to produce p-caryophyllene. Product identification and determination of the activity of purified AaCPS1 were done by gas chromatography-mass spectrometry (GC-MS). GC-MS results revealed that the AaCPS1 produced maximum 26.5 +/- 1 mg of P-caryophyllene per kilogram fresh weight of leaves after assaying with FPP for 6 h. Using AaCPS1 as a proof of concept, we demonstrate that N. benthamiana can be considered as an expression system for production of plant proteins that catalyze the formation of valuable chemicals for industrial applications.

Place, publisher, year, edition, pages
PeerJ Inc, 2020
Keywords
Artemisia annua, Sesquiterpene synthase, AaCPS1, Phylogenetics, Terpenoids, Nicotiana benthamiana, Transient expression, beta-caryophyllene synthase
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-94816 (URN)10.7717/peerj.8904 (DOI)000529067400002 ()32377446 (PubMedID)2-s2.0-85085929840 (Scopus ID)
Available from: 2020-05-18 Created: 2020-05-18 Last updated: 2023-08-28Bibliographically approved
Guo, M., Hu, Y., Wang, L., Brodelius, P. E. & Sun, L. (2018). A facile synthesis of molecularly imprinted polymers and their properties as electrochemical sensors for ethyl carbamate analysis. RSC Advances, 8(69), 39721-39730
Open this publication in new window or tab >>A facile synthesis of molecularly imprinted polymers and their properties as electrochemical sensors for ethyl carbamate analysis
Show others...
2018 (English)In: RSC Advances, E-ISSN 2046-2069, Vol. 8, no 69, p. 39721-39730Article in journal (Refereed) Published
Abstract [en]

New molecularly imprinted polymers (MIPs), which exhibit specific recognition of ethyl carbamate (EC) have been synthesized and studied. In this process, EC was the template molecule and -cyclodextrin derivatives were employed as functional monomers in the molecular imprinting technique (MIT). An EC molecularly imprinted sensor (EC-MIS) was prepared by using MIT surface modification. The EC-MIS was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry. EC detection performance, binding parameters and dynamics mechanism were investigated. The result showed that the synthetic route designed was appropriate and that new MIP and EC-MIS were successfully prepared. The EC-MIS exhibited a good molecular recognition of EC. A linear relationship between current and EC concentration was observed using cyclic voltammetry and the detection limit was 5.86 g L-1. The binding constant (K = 4.75 x 10(6) L mol(-1)) between EC and the EC-MIS, as well as, the number of binding sites (n = 1.48) has been determined. The EC-MIS recognition mechanism for the EC is a two-step process. The sensor was applied for the determination of EC in Chinese yellow wines, and the results were in good agreement with the gas chromatography-mass spectrometry (GC-MS) method.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2018
National Category
Organic Chemistry
Research subject
Chemistry, Organic Chemistry
Identifiers
urn:nbn:se:lnu:diva-79616 (URN)10.1039/c8ra08213h (DOI)000452116300055 ()2-s2.0-85057761823 (Scopus ID)
Available from: 2019-01-17 Created: 2019-01-17 Last updated: 2022-09-15Bibliographically approved
Shen, Q., Zhang, L., Liao, Z., Wang, S., Yan, T., Shi, P., . . . Tang, K. (2018). The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis. Molecular Plant, 11(6), 776-788
Open this publication in new window or tab >>The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis
Show others...
2018 (English)In: Molecular Plant, ISSN 1674-2052, E-ISSN 1752-9867, Vol. 11, no 6, p. 776-788Article in journal (Refereed) Published
Abstract [en]

Artemisia annua, commonly known as sweet wormwood or Qinghao, is a shrub native to China and has long been used for medicinal purposes. A. annua is now cultivated globally as the only natural source of a potent anti-malarial compound, artemisinin. Here, we report a high-quality draft assembly of the 1.74-gigabase genome of A. annua, which is highly heterozygous, rich in repetitive sequences, and contains 63 226 protein-coding genes, one of the largest numbers among the sequenced plant species. We found that, as one of a few sequenced genomes in the Asteraceae, the A. annua genome contains a large number of genes specific to this large angiosperm clade. Notably, the expansion and functional diversification of genes encoding enzymes involved in terpene biosynthesis are consistent with the evolution of the artemisinin biosynthetic pathway. We further revealed by transcriptome profiling that A. annua has evolved the sophisticated transcriptional regulatory networks underlying artemisinin biosynthesis. Based on comprehensive genomic and transcriptomic analyses we generated transgenic A. annua lines producing high levels of artemisinin, which are now ready for large-scale production and thereby will help meet the challenge of increasing global demand of artemisinin.

Place, publisher, year, edition, pages
Cell Press, 2018
Keywords
Artemisia annua, artemisinin, genome, evolution, transcriptome, metabolic engineering
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-76879 (URN)10.1016/j.molp.2018.03.015 (DOI)000434429000003 ()29703587 (PubMedID)2-s2.0-85045897546 (Scopus ID)
Available from: 2018-07-17 Created: 2018-07-17 Last updated: 2020-10-20Bibliographically approved
Matias-Hernandez, L., Jiang, W., Yang, K., Tang, K., Brodelius, P. E. & Pelaz, S. (2017). AaMYB1 and its orthologue AtMYB61 affect terpene metabolism and trichome development in Artemisia annua and Arabidopsis thaliana. The Plant Journal, 90(3), 520-534
Open this publication in new window or tab >>AaMYB1 and its orthologue AtMYB61 affect terpene metabolism and trichome development in Artemisia annua and Arabidopsis thaliana
Show others...
2017 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 90, no 3, p. 520-534Article in journal (Refereed) Published
Abstract [en]

The effective anti-malarial drug artemisinin (AN) isolated from Artemisia annua is relatively expensive due to the low AN content in the plant as AN is only synthesized within the glandular trichomes. Therefore, genetic engineering of A. annua is one of the most promising approaches for improving the yield of AN. In this work, the AaMYB1 transcription factor has been identified and characterized. When AaMYB1 is overexpressed in A. annua, either exclusively in trichomes or in the whole plant, essential AN biosynthetic genes are also overexpressed and consequently the amount of AN is significantly increased. Artemisia AaMYB1 constitutively overexpressing plants displayed a greater number of trichomes. In order to study the role of AaMYB1 on trichome development and other possibly connected biological processes, AaMYB1 was overexpressed in Arabidopsis thaliana. To support our findings in Arabidopsis thaliana, an AaMYB1 orthologue from this model plant, AtMYB61, was identified and atmyb61 mutants characterized. Both AaMYB1 and AtMYB61 affected trichome initiation, root development and stomatal aperture in A. thaliana. Molecular analyses indicated that two crucial trichome activator genes are misexpressed in atmyb61 mutant plants and in plants overexpressing AaMYB1. Furthermore, AaMYB1 and AtMYB61 are also essential for gibberellin (GA) biosynthesis and degradation in both species by positively affecting the expression of the enzymes that convert GA(9) into the bioactive GA(4) as well as the enzymes involved in the degradation of GA(4). Overall, these results identify AaMYB1/AtMYB61 as a key component of the molecular network that connects important biosynthetic processes, and reveal its potential value for AN production through genetic engineering.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2017
Keywords
artemisinin, gibberellin, MYB transcription factors, trichome, Artemisia annua, Arabidopsis thaliana
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-64356 (URN)10.1111/tpj.13509 (DOI)000399732200007 ()28207974 (PubMedID)2-s2.0-85016399868 (Scopus ID)
Available from: 2017-05-24 Created: 2017-05-24 Last updated: 2019-08-29Bibliographically approved
Guo, M., Lu, X., Wang, Y. & Brodelius, P. E. (2017). Comparison of the interaction between lactoferrin and isomeric drugs. Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, 173, 593-607
Open this publication in new window or tab >>Comparison of the interaction between lactoferrin and isomeric drugs
2017 (English)In: Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, ISSN 1386-1425, E-ISSN 1873-3557, Vol. 173, p. 593-607Article in journal (Refereed) Published
Abstract [en]

The binding properties of pentacyclic triterpenoid isomeric drugs, i.e. ursolic acid (UA) and oleanolic acid (OA), to bovine lactoferrin (BLF) have been studied by molecule modeling, fluorescence spectroscopy, UV-visible absorbance spectroscopy and infrared spectroscopy (IR). Molecular docking, performed to reveal the possible binding mode or mechanism, suggested that hydrophobic interaction and hydrogen bonding play important roles to stabilize the complex. The results of spectroscopic measurements showed that the two isomeric drugs both strongly quenched the intrinsic fluorescence of BLF through a static quenching procedure although some differences between UM and OA binding strength and non-radiation energy transfer occurred within the molecules. The number of binding sites was 3.44 and 3.10 for UA and OA, respectively, and the efficiency of Forster energy transfer provided a distance of 0.77 and 1.21 nm for UA and OA, respectively. The conformation transformation of BLF affected by the drugs conformed to the "all-or-none" pattern. In addition, the changes of the ratios of alpha-helices, beta-sheets and beta-turns of BLF during the process of the interaction were obtained. The results of the experiments in combination with the calculations showed that there are two modes of pentacyclic triterpenoid binding to BLF instead of one binding mode only governed by the principle of the lowest bonding energy.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Ursolic acid, Oleanolic acid, Bovine lactoferrin, Interaction
National Category
Analytical Chemistry Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
urn:nbn:se:lnu:diva-60150 (URN)10.1016/j.saa.2016.10.029 (DOI)000390502900082 ()2-s2.0-84992376464 (Scopus ID)
Available from: 2017-01-24 Created: 2017-01-24 Last updated: 2019-09-09Bibliographically approved
Guo, M., Wang, X., Lu, X., Wang, H. & Brodelius, P. E. (2016). alpha-Mangostin Extraction from the Native Mangosteen (Garcinia mangostana L.) and the Binding Mechanisms of alpha-Mangostin to HSA or TRF. PLOS ONE, 11(9), Article ID e0161566.
Open this publication in new window or tab >>alpha-Mangostin Extraction from the Native Mangosteen (Garcinia mangostana L.) and the Binding Mechanisms of alpha-Mangostin to HSA or TRF
Show others...
2016 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 11, no 9, article id e0161566Article in journal (Refereed) Published
Abstract [en]

In order to obtain the biological active compound, alpha-mangostin, from the traditional native mangosteen (Garcinia mangostana L.), an extraction method for industrial application was explored. A high yield of a-mangostin (5.2%) was obtained by extraction from dried mangosteen pericarps with subsequent purification on macroporous resin HPD-400. The chemical structure of alpha-mangostin was verified mass spectrometry (MS), nuclear magnetic resonance (H-1 NMR and C-13 NMR), infrared spectroscopy (IR) and UV-Vis spectroscopy. The purity of the obtained alpha-mangostin was 95.6% as determined by HPLC analysis. The binding of native alpha-mangostin to human serum albumin (HSA) or transferrin (TRF) was explored by combining spectral experiments with molecular modeling. The results showed that amangostin binds to HSA or TRF as static complexes but the binding affinities were different in different systems. The binding constants and thermodynamic parameters were measured by fluorescence spectroscopy and absorbance spectra. The association constant of HSA or TRF binding to alpha-mangostin is 6.4832x10(5) L/mol and 1.4652x10(5) L/mol at 298 K and 7.8619x10(5) L/mol and 1.1582x10(5) L/mol at 310 K, respectively. The binding distance, the energy transfer efficiency between alpha-mangostin and HSA or TRF were also obtained by virtue of the Forster theory of non-radiation energy transfer. The effect of alpha-mangostin on the HSA or TRF conformation was analyzed by synchronous spectrometry and fluorescence polarization studies. Molecular docking results reveal that the main interaction between amangostin and HSA is hydrophobic interactions, while the main interaction between alpha-mangostin and TRF is hydrogen bonding and Van der Waals forces. These results are consistent with spectral results.

National Category
Organic Chemistry
Research subject
Chemistry, Organic Chemistry
Identifiers
urn:nbn:se:lnu:diva-57613 (URN)10.1371/journal.pone.0161566 (DOI)000382855600041 ()2-s2.0-84990831662 (Scopus ID)
Available from: 2016-10-26 Created: 2016-10-25 Last updated: 2021-06-14Bibliographically approved
Liu, M., Shi, P., Fu, X., Brodelius, P. E., Shen, Q., Jiang, W., . . . Tang, K. (2016). Characterization of a trichome-specific promoter of the aldehyde dehydrogenase 1 (ALDH1) gene in Artemisia annua. Plant Cell Tissue and Organ Culture, 126(3), 469-480
Open this publication in new window or tab >>Characterization of a trichome-specific promoter of the aldehyde dehydrogenase 1 (ALDH1) gene in Artemisia annua
Show others...
2016 (English)In: Plant Cell Tissue and Organ Culture, ISSN 0167-6857, E-ISSN 1573-5044, Vol. 126, no 3, p. 469-480Article in journal (Refereed) Published
Abstract [en]

Artemisinin is a frequently used anti-malaria drug extracted from glandular trichomes (GSTs) in Artemisia annua L. In this study, we report on the characterization of the promoter of aldehyde dehydrogenase 1 (ALDH1) involved in the biosynthesis of artemisinin. A 1620-bp promoter fragment was cloned upstream of the ALDH1 start codon. Putative regulatory cis-acting elements are predicted by software, revealing that this gene is affected by complex factors. The activity of the ALDH1 promoter was analyzed using a reporter gene GUS. GUS expression showed a spatial difference in leaves at different ages. In young leaves, GUS staining was exclusively discovered in GSTs. In older leaves, both GSTs and T-shaped trichomes (TSTs) showed GUS signals. Only TSTs showed GUS staining in lower leaves. No GUS staining was detected in the bottom leaves. The result demonstrates that the ALDH1 promoter is trichome-specific. The RT-Q-PCR analysis revealed that both wild-type and recombinant promoters showed similar activity in A. annua. After application of exogenous 100 μM methyl jasmonate, 100 μM gibberellin and 100 μM salicylic acid separately, the transcript levels were increased significantly, indicating that ALDH1 may play an important role in the response to hormones in A. annua.

Keywords
Aldehyde dehydrogenase 1, Artemisia annua, Hormonal treatment, Promoter, Transgene
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biotechnology
Identifiers
urn:nbn:se:lnu:diva-56071 (URN)10.1007/s11240-016-1015-4 (DOI)000382151200009 ()2-s2.0-84983050078 (Scopus ID)
Available from: 2016-09-16 Created: 2016-08-31 Last updated: 2017-11-21Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-8899-5046

Search in DiVA

Show all publications