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Rosengren-Holmberg, Jenny P.
Alternative names
Publications (10 of 40) Show all publications
Rosengren-Holmberg, J. P., Andersson, J., Smith, J. R., Alexander, C., Alexander, M. R., Tovar, G., . . . Nicholls, I. A. (2015). Heparin molecularly imprinted surfaces for the attenuation of complement activation in blood. Biomaterials Science, 3(8), 1208-1217
Open this publication in new window or tab >>Heparin molecularly imprinted surfaces for the attenuation of complement activation in blood
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2015 (English)In: Biomaterials Science, ISSN 2047-4830, E-ISSN 2047-4849, Vol. 3, no 8, p. 1208-1217Article in journal (Refereed) Published
Abstract [en]

Heparin-imprinted synthetic polymer surfaces with the ability to attenuate activation of both the complement and the coagulation system in whole blood were successfully produced. Imprinting was achieved using a template coated with heparin, a highly sulfated glycosaminoglycan known for its anticoagulant properties. The N,N'-diacryloylpiperazine-methacrylic acid copolymers were characterized using goniometry, AFM and XPS. The influence of the molecular imprinting process on morphology and template rebinding was demonstrated by radioligand binding assays. Surface hemocompatibility was evaluated using human whole blood without anticoagulants followed by measurement of complement activation markers C3a and sC5b-9 and platelet consumption as a surrogate coagulation activation marker. The observed low thrombogenicity of this copolymer combined with the attenuation of complement activation induced by the molecular imprint offer potential for the development of self-regulating surfaces with important potential clinical applications. We propose a mechanism for the observed phenomena based upon the recruitment of endogenous sulfated glycosaminoglycans with heparin-like activities.

National Category
Biological Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-45799 (URN)10.1039/c5bm00047e (DOI)000357937400004 ()26222036 (PubMedID)2-s2.0-84937199587 (Scopus ID)
Available from: 2015-08-20 Created: 2015-08-19 Last updated: 2018-11-02Bibliographically approved
Engberg, A. E., Nilsson, P. H., Huang, S., Fromell, K., Hamad, O. A., Mollnes, T. E., . . . Nilsson Ekdahl, K. (2015). Prediction of inflammatory responses induced by biomaterials in contact with human blood using protein fingerprint from plasma. Biomaterials, 36, 55-65
Open this publication in new window or tab >>Prediction of inflammatory responses induced by biomaterials in contact with human blood using protein fingerprint from plasma
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2015 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 36, p. 55-65Article in journal (Refereed) Published
Abstract [en]

Inappropriate complement activation is often responsible for incompatibility reactions that occur when biomaterials are used. Complement activation is therefore a criterion included in legislation regarding biomaterials testing. However, no consensus is yet available regarding appropriate complement-activation-related test parameters. We examined protein adsorption in plasma and complement activation/cytokine release in whole blood incubated with well-characterized polymers. Strong correlations were found between the ratio of C4 to its inhibitor C4BP and generation of 10 (mainly pro-inflammatory) cytokines, including IL-17, IFN-gamma, and IL-6. The levels of complement activation products correlated weakly (C3a) or not at all (C5a, sC5b-9), confirming their poor predictive values. We have demonstrated a direct correlation between downstream biological effects and the proteins initially adhering to an artificial surface after contact with blood. Consequently, we propose the C4/C4BP ratio as a robust, predictor of biocompatibility with superior specificity and sensitivity over the current gold standard. (C) 2014 Elsevier Ltd. All rights reserved.

Keywords
Biomaterials, C4 binding protein (C4BP), Complement, Cytokines, Inflammation, In vitro screening
National Category
Immunology Biomaterials Science
Research subject
Chemistry, Organic Chemistry; Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-39124 (URN)10.1016/j.biomaterials.2014.09.011 (DOI)000345728200006 ()2-s2.0-84921965003 (Scopus ID)
Available from: 2015-01-15 Created: 2015-01-15 Last updated: 2018-11-02Bibliographically approved
Engberg, A. E., Nilsson, P. H., Mollnes, T. E., Rosengren-Holmberg, J. P., Nicholls, I. A., Nilsson, B. & Nilsson Ekdahl, K. (2012). The ratio between C4 and C4BP adsorbed from plasma predicts cytokine generation induced by artificial polymers in contact with whole blood. Immunobiology, 217(11), 1211-1211
Open this publication in new window or tab >>The ratio between C4 and C4BP adsorbed from plasma predicts cytokine generation induced by artificial polymers in contact with whole blood
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2012 (English)In: Immunobiology, ISSN 0171-2985, E-ISSN 1878-3279, Vol. 217, no 11, p. 1211-1211Article in journal, Meeting abstract (Other academic) Published
National Category
Immunology
Research subject
Biomedical Sciences, Immunology
Identifiers
urn:nbn:se:lnu:diva-23358 (URN)10.1016/j.imbio.2012.08.235 (DOI)000311187800247 ()
Available from: 2013-01-09 Created: 2013-01-09 Last updated: 2017-12-06Bibliographically approved
Nilsson Ekdahl, K., Engberg, A. E., Rosengren-Holmberg, J. P., Chen, H., Lambris, J. & Nicholls, I. A. (2011). Blood protein-polymer adsorption fingerprinting: Implications for understanding hemoocompatibility and for biomaterial design.. Journal of Biomedical Materials Research. Part A, 97A(1), 74-84
Open this publication in new window or tab >>Blood protein-polymer adsorption fingerprinting: Implications for understanding hemoocompatibility and for biomaterial design.
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2011 (English)In: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, E-ISSN 1552-4965, Vol. 97A, no 1, p. 74-84Article in journal (Refereed) Published
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-10057 (URN)10.1002/jbm.a.33030 (DOI)2-s2.0-79951972598 (Scopus ID)
Available from: 2011-01-17 Created: 2011-01-17 Last updated: 2017-12-11Bibliographically approved
Nicholls, I. A., Karlsson, B. C. G., Andersson, H. S., Golker, K., Henschel, H., Olsson, G. D., . . . Wikman, S. (2009). Biomimetic Polymer Design. In: : . Paper presented at Scanbalt Forum Biomaterials Days, Kalmar.
Open this publication in new window or tab >>Biomimetic Polymer Design
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2009 (English)Conference paper, Published paper (Refereed)
National Category
Chemical Sciences
Research subject
Chemistry, Organic Chemistry
Identifiers
urn:nbn:se:lnu:diva-6756 (URN)
Conference
Scanbalt Forum Biomaterials Days, Kalmar
Available from: 2010-07-08 Created: 2010-07-08 Last updated: 2018-05-18Bibliographically approved
Rosengren-Holmberg, J. P., Karlsson, J. G., Svenson, J. & Nicholls, I. A. (2009). Paracetamol selective polymers prepared by semi-covalent and non-covalent imprinting. In: : . Paper presented at Scanbalt Forum Biomaterials Days, Kalmar.
Open this publication in new window or tab >>Paracetamol selective polymers prepared by semi-covalent and non-covalent imprinting
2009 (English)Conference paper, Published paper (Refereed)
Identifiers
urn:nbn:se:lnu:diva-6758 (URN)
Conference
Scanbalt Forum Biomaterials Days, Kalmar
Available from: 2010-07-08 Created: 2010-07-08 Last updated: 2015-04-16Bibliographically approved
Rosengren-Holmberg, J. P., Karlsson, J. G., Svenson, J., Andersson, H. S. & Nicholls, I. A. (2009). Synthesis and ligand recognition of paracetamol selective polymers: semi-covalent versus non-covalent molecular imprinting.. Organic and biomolecular chemistry, 7, 3148-3155
Open this publication in new window or tab >>Synthesis and ligand recognition of paracetamol selective polymers: semi-covalent versus non-covalent molecular imprinting.
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2009 (English)In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 7, p. 3148-3155Article in journal (Refereed) Published
Abstract [en]

Three molecular imprinting strategies, each based upon a series of ethylene glycol dimethacrylate (EGDMA) cross-linked co-polymers, have been used to produce materials selective for the commonly used analgesic and antipyretic agent paracetamol (p-acetaminophen or 4-acetamidophenol) (1). The polymers were synthesised using either a semi-covalent imprinting strategy based upon 4-acetamidophenyl-(4-vinylphenyl) carbonate (4) or a non-covalent strategy based on methacrylic acid (MAA) as the functional monomer, or by employing a combination of these strategies. Radioligand binding studies demonstrated low template affinity in polymers offering only a single electrostatic interaction point for recognition via the phenolic residue in the template, whereas binding was substantially increased upon the introduction of a second binding mode, namely interaction at the acetamide moiety. HPLC analyses revealed no imprinting effect in the purely semi-covalent system, and only a minor effect in the purely non-covalent systems. However, a pronounced imprinting effect was demonstrated for polymers prepared by a combination of semi-covalent and non-covalent imprinting. This study illustrates a limitation of both the non-covalent and the semi-covalent strategies when it comes to achieving imprinted selectivity for small and poorly functionalised templates such as paracetamol. Parallels with conclusions from studies with antibodies are discussed. 

National Category
Organic Chemistry
Research subject
Chemistry, Organic Chemistry
Identifiers
urn:nbn:se:lnu:diva-7870 (URN)10.1039/b900014c (DOI)
Available from: 2010-08-27 Created: 2010-08-27 Last updated: 2017-12-12Bibliographically approved
Engberg, A. E., Rosengren-Holmberg, J. P., Nilsson Ekdahl, K. & Nicholls, I. A. (2009). Synthesis of new polymers and evaluation of their hemocompatibility. In: : . Paper presented at Scanbalt Forum Biomaterials Days, Kalmar.
Open this publication in new window or tab >>Synthesis of new polymers and evaluation of their hemocompatibility
2009 (English)Conference paper, Published paper (Refereed)
Identifiers
urn:nbn:se:lnu:diva-6754 (URN)
Conference
Scanbalt Forum Biomaterials Days, Kalmar
Available from: 2010-07-08 Created: 2010-07-08 Last updated: 2016-11-10Bibliographically approved
Engberg, A. E., Rosengren-Holmberg, J., Nicholls, I. A. & Nilsson Ekdahl, K. (2008). Development of novel biomaterial surfaces and evaluation of their hemocompatibility*. In: Journal of Molecular Immunology 45: .
Open this publication in new window or tab >>Development of novel biomaterial surfaces and evaluation of their hemocompatibility*
2008 (English)In: Journal of Molecular Immunology 45, 2008Conference paper, Published paper (Refereed)
Research subject
Biomedical Sciences, Immunology; Chemistry, Organic Chemistry
Identifiers
urn:nbn:se:lnu:diva-5055 (URN)
Note

Nummer:

Available from: 2010-04-28 Created: 2010-04-28 Last updated: 2016-11-10Bibliographically approved
Engberg, A. E., Rosengren-Holmberg, J., Nicholls, I. A. & Nilsson Ekdahl, K. (2008). Development of novel biomaterial surfaces and evaluation of their hemocompatibility. In: : . Paper presented at 22nd International Complement Workshop. Basel, Switzerland, September 28-October 2
Open this publication in new window or tab >>Development of novel biomaterial surfaces and evaluation of their hemocompatibility
2008 (English)Conference paper, Published paper (Refereed)
Place, publisher, year, edition, pages
Basel, Switzerland, September 28-October 2: , 2008
Research subject
Chemistry, Organic Chemistry
Identifiers
urn:nbn:se:lnu:diva-4980 (URN)
Conference
22nd International Complement Workshop
Note

Nummer:

Available from: 2010-04-28 Created: 2010-04-28 Last updated: 2016-11-10Bibliographically approved
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