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Axelsson Olsson, Diana
Publications (10 of 20) Show all publications
Olofsson, J., Griekspoor, P., Olsen, B., Ellström, P. & Axelsson Olsson, D. (2015). The abundant free-living amoeba, Acanthamoeba polyphaga, increases the survival of Campylobacter jejuni in milk and orange juice. Infection Ecology & Epidemiology, 5, Article ID 28675.
Open this publication in new window or tab >>The abundant free-living amoeba, Acanthamoeba polyphaga, increases the survival of Campylobacter jejuni in milk and orange juice
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2015 (English)In: Infection Ecology & Epidemiology, ISSN 2000-8686, E-ISSN 2000-8686, Vol. 5, article id 28675Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Campylobacter jejuni is a common cause of human bacterial diarrhea in most parts of the world. Most C. jejuni infections are acquired from contaminated poultry, milk, and water. Due to health care costs and human suffering, it is important to identify all possible sources of infection. Unpasteurized milk has been associated with several outbreaks of C. jejuni infection. Campylobacter has been identified on fresh fruit, and other gastrointestinal pathogens such as Salmonella, E. coli O157:H7 and Cryptosporidium have been involved in fruit juice outbreaks. C. jejuni is sensitive to the acidic environment of fruit juice, but co-cultures with the amoeba, Acanthamoeba polyphaga, have previously been shown to protect C. jejuni at low pH.

METHODS: To study the influence of A. polyphaga on the survival of C. jejuni in milk and juice, the bacteria were incubated in the two products at room temperature and at 4°C with the following treatments: A) C. jejuni preincubated with A. polyphaga before the addition of product, B) C. jejuni mixed with A. polyphaga after the addition of product, and C) C. jejuni in product without A. polyphaga. Bacterial survival was assessed by colony counts on blood agar plates.

RESULTS: Co-culture with A. polyphaga prolonged the C. jejuni survival both in milk and juice. The effect of co-culture was most pronounced in juice stored at room temperature. On the other hand, A. polyphaga did not have any effect on C. jejuni survival during pasteurization of milk or orange juice, indicating that this is a good method for eliminating C. jejuni in these products.

CONCLUSION: Amoebae-associated C. jejuni in milk and juice might cause C. jejuni infections.

Keywords
unpasteurized milk, fruit juice, C. jejuni infection, co-culture, Campylobacter survival, gastrointestinal pathogens
National Category
Infectious Medicine Microbiology in the medical area
Research subject
Ecology, Microbiology; Natural Science, Medicine
Identifiers
urn:nbn:se:lnu:diva-51507 (URN)10.3402/iee.v5.28675 (DOI)26387556 (PubMedID)
Available from: 2016-03-29 Created: 2016-03-29 Last updated: 2018-01-10Bibliographically approved
Axelsson Olsson, D. (2014). Interaktioner mellan bakterier och protozoer i vattenmiljö, ett dolt hot?. In: : . Paper presented at Mikrobiologiskt vårmöte 2014, Trollhättan, 9-11 april, Sweden. Invited speaker..
Open this publication in new window or tab >>Interaktioner mellan bakterier och protozoer i vattenmiljö, ett dolt hot?
2014 (Swedish)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
urn:nbn:se:lnu:diva-55456 (URN)
Conference
Mikrobiologiskt vårmöte 2014, Trollhättan, 9-11 april, Sweden. Invited speaker.
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2017-01-18Bibliographically approved
Olofsson, J., Axelsson Olsson, D., Brudin, L., Olsen, B. & Ellström, P. (2013). Campylobacter jejuni Actively Invades the Amoeba Acanthamoeba polyphaga and Survives within Non Digestive Vacuoles. PLoS ONE, 8(11), Article ID e78873.
Open this publication in new window or tab >>Campylobacter jejuni Actively Invades the Amoeba Acanthamoeba polyphaga and Survives within Non Digestive Vacuoles
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 11, article id e78873Article in journal (Refereed) Published
Abstract [en]

The Gram-negative bacterium Campylobacter jejuni is able to enter, survive and multiply within the free living amoeba Acanthamoeba polyphaga, but the molecular mechanisms behind these events are still unclear. We have studied the uptake and intracellular trafficking of viable and heat killed bacterial cells of the C. jejuni strain 81-176 in A. polyphaga. We found that viable bacteria associated with a substantially higher proportion of Acanthamoeba trophozoites than heat killed bacteria. Furthermore, the kinetics of internalization, the total number of internalized bacteria as well as the intracellular localization of internalized C. jejuni were dramatically influenced by bacterial viability. Viable bacteria were internalized at a high rate already after 1 h of co-incubation and were observed in small vacuoles tightly surrounding the bacteria. In contrast, internalization of heat killed C. jejuni was low at early time points and did not peak until 96 h. These cells were gathered in large spacious vacuoles that were part of the degradative pathway as determined by the uptake of fluorescently labeled dextran. The amount of heat killed bacteria internalized by A. polyphaga did never reach the maximal amount of internalized viable bacteria. These results suggest that the uptake and intracellular survival of C. jejuni in A. polyphaga is bacterially induced.

National Category
Microbiology
Research subject
Ecology, Zoonotic Ecology
Identifiers
urn:nbn:se:lnu:diva-30994 (URN)10.1371/journal.pone.0078873 (DOI)000326656200061 ()2-s2.0-84892404372 (Scopus ID)
Available from: 2013-12-06 Created: 2013-12-06 Last updated: 2017-12-06Bibliographically approved
Griekspoor, P., Olofsson, J., Axelsson Olsson, D., Waldenström, J. & Olsen, B. (2013). Multilocus Sequence Typing and FlaA Sequencing Reveal the Genetic Stability of Campylobacter jejuni Enrichment during Coculture with Acanthamoeba polyphaga. Applied and Environmental Microbiology, 79(7), 2477-2479
Open this publication in new window or tab >>Multilocus Sequence Typing and FlaA Sequencing Reveal the Genetic Stability of Campylobacter jejuni Enrichment during Coculture with Acanthamoeba polyphaga
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2013 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 79, no 7, p. 2477-2479Article in journal (Refereed) Published
Abstract [en]

Low concentrations of Campylobacter jejuni cells in environmental samples make them difficult to study with conventional culture methods. Here, we show that enrichment by amoeba cocultures works well with low-concentration samples and that this method can be combined with molecular techniques without loss of genetic specificity.

National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
urn:nbn:se:lnu:diva-25338 (URN)10.1128/AEM.02918-12 (DOI)000316183500046 ()2-s2.0-84875513333 (Scopus ID)
Available from: 2013-04-18 Created: 2013-04-18 Last updated: 2017-12-06Bibliographically approved
Axelsson Olsson, D. (2012). Interaktioner mellan bakterier och protozoer i vattenmiljö, ett dolt hot?. In: : . Paper presented at Veterinärkongressen 2012, 8-9 november, Uppsala, Sweden. Invited speaker.
Open this publication in new window or tab >>Interaktioner mellan bakterier och protozoer i vattenmiljö, ett dolt hot?
2012 (Swedish)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
urn:nbn:se:lnu:diva-55455 (URN)
Conference
Veterinärkongressen 2012, 8-9 november, Uppsala, Sweden. Invited speaker
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2017-04-18Bibliographically approved
Axelsson Olsson, D., Olofsson, J., Svensson, L., Griekspoor, P., Waldenström, J., Ellström, P. & Olsen, B. (2010). Amoebae and algae can prolong the survival of Campylobacter species in co-culture. Experimental parasitology, 126, 59-64
Open this publication in new window or tab >>Amoebae and algae can prolong the survival of Campylobacter species in co-culture
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2010 (English)In: Experimental parasitology, ISSN 0014-4894, E-ISSN 1090-2449, Vol. 126, p. 59-64Article in journal (Refereed) Published
Abstract [en]

Several species of free-living amoebae can cause disease in humans. However, in addition to the direct pathogenicity of e.g. Acanthamoebae and Naegleria species, they are recognized as environmental hosts, indirectly involved in the epidemiology of many pathogenic bacteria. Although several studies have demonstrated intracellular survival of many different bacteria in these species, the extent of such interactions as well as the implications for the epidemiology of the bacterial species involved, are largely unknown and probably underestimated. In this study, we evaluated eight different unicellular eukaryotic organisms, for their potential to serve as environmental hosts for Campylobacter species. These organisms include four amoebozoas (Acanthamoeba polyphaga, Acanthamoeba castellanii, Acanthamoeba rhysodes and Hartmanella vermiformis), one alveolate (Tetrahymena pyriformis), one stramenopile (Dinobryon sertularia), one eugoenozoa (Euglena gracilis) and one heterolobosea (Naegleria americana). Campylobacter spp. including Campylobacter jejuni, Campylobacter coli and Campylobacter lari are the most common cause of gastroenteritis in the western world. Survival and replication of these three species as well as Campylobacter hyointestinalis were assessed in co-cultures with the eukaryotic organisms. Campylobacter spp. generally survived longer in co-cultures, compared to when incubated in the corresponding growth media. The eukaryotic species that best promoted bacterial survival was the golden algae D. sertularia. Three species of amoebozoas, of the genus Acanthamoeba promoted both prolonged survival and replication of Campylobacter spp. The high abundance in lakes, ponds and water distribution networks of these organisms indicate that they might have a role in the epidemiology of campylobacteriosis, possibly contributing to survival and dissemination of these intestinal pathogens to humans and other animals. The results suggest that not only C. jejuni, but a variety of Campylobacter spp. can interact with different eukaryotic unicellular organisms.

National Category
Infectious Medicine
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-7086 (URN)10.1016/j.exppara.2009.12.016 (DOI)000278917000012 ()
Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved
Waldenström, J., Axelsson Olsson, D., Olsen, B., Hasselquist, D., Griekspoor, P., Jansson, L., . . . Ellström, P. (2010). Campylobacter jejuni colonization in wild birds: Results from an infection experiment. PLoS ONE, 5(2), Article ID e9082.
Open this publication in new window or tab >>Campylobacter jejuni colonization in wild birds: Results from an infection experiment
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2010 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 5, no 2, article id e9082Article in journal (Refereed) Published
Abstract [en]

Campylobacter jejuni is a common cause of bacterial gastroenteritis in most parts of the world. The bacterium has a broad host range and has been isolated from many animals and environments. To investigate shedding patterns and putative effects on an avian host, we developed a colonization model in which a wild bird species, the European Robin Erithacus rubecula, was inoculated orally with C. jejuni from either a human patient or from another wild bird species, the Song Thrush Turdus philomelos. These two isolates were genetically distinct from each other and provoked very different host responses. The Song Thrush isolate colonized all challenged birds and colonization lasted 6.8 days on average. Birds infected with this isolate also showed a transient but significant decrease in body mass. The human isolate did not colonize the birds and could be detected only in the feces of the birds shortly after inoculation. European Robins infected with the wild bird isolate generated a specific antibody response to C. jejuni membrane proteins from the avian isolate, which also was cross-reactive to membrane proteins of the human isolate. In contrast, European Robins infected with the human isolate did not mount a significant response to bacterial membrane proteins from either of the two isolates. The difference in colonization ability could indicate host adaptations.

National Category
Microbiology
Research subject
Ecology, Zoonotic Ecology
Identifiers
urn:nbn:se:lnu:diva-7083 (URN)10.1371/journal.pone.0009082 (DOI)2-s2.0-77949373747 (Scopus ID)
Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved
Axelsson Olsson, D., Svensson, L., Olofsson, J., Salomon, P., Waldenström, J., Ellström, P. & Olsen, B. (2010). Increase in Acid Tolerance of Campylobacter jejuni through Coincubation with Amoebae. Applied and Environmental Microbiology, 76(13), 4194-4200
Open this publication in new window or tab >>Increase in Acid Tolerance of Campylobacter jejuni through Coincubation with Amoebae
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2010 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 76, no 13, p. 4194-4200Article in journal (Refereed) Published
Abstract [en]

Campylobacter jejuni is a recognized and common gastrointestinal pathogen in most parts of the world. Human infections are often food borne, and the bacterium is frequent among poultry and other food animals. However, much less is known about the epidemiology of C. jejuni in the environment and what mechanisms the bacterium depends on to tolerate low pH. The sensitive nature of C. jejuni stands in contrast to the fact that it is difficult to eradicate from poultry production, and even more contradictory is the fact that the bacterium is able to survive the acidic passage through the human stomach. Here we expand the knowledge on C. jejuni acid tolerance by looking at protozoa as a potential epidemiological pathway of infection. Our results showed that when C. jejuni cells were coincubated with Acanthamoeba polyphaga in acidified phosphate-buffered saline (PBS) or tap water, the bacteria could tolerate pHs far below those in their normal range, even surviving at pH 4 for 20 h and at pH 2 for 5 h. Interestingly, moderately acidic conditions (pH 4 and 5) were shown to trigger C. jejuni motility as well as to increase adhesion/internalization of bacteria into A. polyphaga. Taken together, the results suggest that protozoa may act as protective hosts against harsh conditions and might be a potential risk factor for C. jejuni infections. These findings may be important for our understanding of C. jejuni passage through the gastrointestinal tract and for hygiene practices used in poultry settings.

National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
urn:nbn:se:lnu:diva-7087 (URN)10.1128/AEM.01219-09 (DOI)2-s2.0-77954258414 (Scopus ID)
Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved
Axelsson Olsson, D., Olofsson, J., Ellström, P., Waldenström, J. & Olsen, B. (2009). A simple method for long-term storage Acanthamoeba species. Parasitology Research, 104(4), 935-937
Open this publication in new window or tab >>A simple method for long-term storage Acanthamoeba species
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2009 (English)In: Parasitology Research, ISSN 0932-0113, E-ISSN 1432-1955, Vol. 104, no 4, p. 935-937Article in journal (Refereed) Published
Abstract [en]

We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyphaga and 3 years for Acanthamoeba castellanii and Acanthamoeba rhysodes. Advantages of this storage method are: (1) it is quick and simple, (2) inexpensive, (3) does not require encystment before storage, (4) resuscitation of cysts can be achieved within a week of culture in PYG medium at 27A degrees C, and does not require co-culture with bacteria or any special equipment.

National Category
Microbiology
Research subject
Ecology, Zoonotic Ecology; Ecology, Microbiology; Biomedical Sciences, Virology
Identifiers
urn:nbn:se:lnu:diva-1912 (URN)10.1007/s00436-008-1304-x (DOI)
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2017-12-12Bibliographically approved
Axelsson Olsson, D. (2009). Campylobacter jejuni acid tolerance increases when co-incubated with amoebae. In: : . Paper presented at FEMS, 3rd congress of European microbiologists, Gothenburg, Sweden, June 28-July 2, 2009.
Open this publication in new window or tab >>Campylobacter jejuni acid tolerance increases when co-incubated with amoebae
2009 (English)Conference paper, Oral presentation with published abstract (Refereed)
National Category
Natural Sciences
Identifiers
urn:nbn:se:lnu:diva-55453 (URN)
External cooperation:
Conference
FEMS, 3rd congress of European microbiologists, Gothenburg, Sweden, June 28-July 2, 2009
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2016-08-25Bibliographically approved
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