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Investigation of viable taxa in the deep terrestrial biosphere suggests high rates of nutrient recycling
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Helmholtz Zentrum Dresden Rossendorf, Germany. (Ctr Ecol & Evolut Microbial Model Syst EEMiS)ORCID iD: 0000-0003-3588-6676
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (Ctr Ecol & Evolut Microbial Model Syst EEMiS)ORCID iD: 0000-0001-9005-5168
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (Ctr Ecol & Evolut Microbial Model Syst EEMiS)
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Univ Copenhagen, Denmark. (Ctr Ecol & Evolut Microbial Model Syst EEMiS)
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2018 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 94, no 8, article id fiy121Article in journal (Refereed) Published
Abstract [en]

The deep biosphere is the largest 'bioreactor' on earth, and microbes inhabiting this biome profoundly influence global nutrient and energy cycles. An important question for deep biosphere microbiology is whether or not specific populations are viable. To address this, we used quantitative PCR and high throughput 16S rRNA gene sequencing of total and viable cells (i.e. with an intact cellular membrane) from three groundwaters with different ages and chemical constituents. There were no statistically significant differences in 16S rRNA gene abundances and microbial diversity between total and viable communities. This suggests that populations were adapted to prevailing oligo trophic conditions and that non-viable cells are rapidly degraded and recycled into new biomass. With higher concentrations of organic carbon, the modem marine and undefined mixed waters hosted a community with a larger range of predicted growth strategies than the ultra-oligo trophic old saline water. These strategies included fermentative and potentially symbiotic lifestyles by candidate phyla that typically have streamlined genomes. In contrast, the old saline waters had more 16S rRNA gene sequences in previously cultured lineages able to oxidize hydrogen and fix carbon dioxide. This matches the paradigm of a hydrogen and carbon dioxide-fed chemolithoauto trophic deep biosphere.

Place, publisher, year, edition, pages
Oxford University Press, 2018. Vol. 94, no 8, article id fiy121
Keywords [en]
16S rRNA gene, deep subsurface, fracture groundwaters, propidium monoazide, viable cells, candidate phyla radiation
National Category
Microbiology Ecology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-77378DOI: 10.1093/femsec/fiy121ISI: 000441198800016PubMedID: 29931252Scopus ID: 2-s2.0-85054565006OAI: oai:DiVA.org:lnu-77378DiVA, id: diva2:1242519
Available from: 2018-08-28 Created: 2018-08-28 Last updated: 2019-08-29Bibliographically approved

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Lopez-Fernandez, MargaritaBroman, EliasTurner, StephanieWu, XiaofenDopson, Mark

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