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Evaluation of a radioprotein-binding assay (RPBA) for folate analysis in berries and milk
Swedish University of Agricultural Sciences.
Swedish University of Agricultural Sciences.
Swedish University of Agricultural Sciences.ORCID-id: 0000-0003-0387-4312
Swedish University of Agricultural Sciences.
2002 (engelsk)Inngår i: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 79, nr 4, s. 525-534Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

This study aimed to optimise a commercial radioprotein-binding assay (RPBA), routinely used for clinical samples, for folate quantification in foods containing mainly 5-CH3-H(4)folate. The assay was modified using external calibration with 5-CH3-H(4)folate in a lower concentration range diluted in food extraction buffer, rather than the buffer with human serum albumin (HSA) provided by the kit. We evaluated the modified RBPA on some selected food products; milk, whey powder, rose hips, strawberries and European certified reference materials (CRM 421, 485) and the adjustments did not affect the assay negatively. Performance parameters included control of selectivity, absence of matrix effects, recoveries of 94-113%, precision of 1-6 CV% (intra-assay) and 5-9 CV% (inter-assay). Folate concentrations in berries and milk, obtained by the modified RPBA were also compared with other quantification methods such as HPLC and MA. The optimised RPBA was found to be a quick and inexpensive complement to HPLC methods, reliable for folate quantification in foods such as milk and berries that contain mainly 5-CH3-H(4)folate. (C) 2002 Elsevier Science Ltd. All rights reserved.

sted, utgiver, år, opplag, sider
2002. Vol. 79, nr 4, s. 525-534
Emneord [en]
5-CH3-H(4)folate, radioprotein-binding assay, quality control, method evaluation, food analysis, berries, milk
HSV kategori
Forskningsprogram
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:lnu:diva-61261DOI: 10.1016/S0308-8146(02)00392-8ISI: 000179308300017OAI: oai:DiVA.org:lnu-61261DiVA, id: diva2:1081603
Tilgjengelig fra: 2017-03-14 Laget: 2017-03-14 Sist oppdatert: 2017-11-29bibliografisk kontrollert

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