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A model to study complement involvement in experimental retinal degeneration.
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB). (Linnaeus Ctr Biomat Chem, BMC)
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB). (Linnaeus Ctr Biomat Chem, BMC)
Karolinska institutet.
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB). Uppsala university. (Linnaeus Ctr Biomat Chem, BMC)ORCID-id: 0000-0001-7888-1571
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2018 (engelsk)Inngår i: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 123, nr 1, s. 28-42Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

BACKGROUND: The complement system (CS) plays a role in the pathogenesis of a number of ocular diseases, including diabetic retinopathy (DR), glaucoma, uveitis, and age-related macular degeneration (AMD). Given that many of the complex eye-related degenerative diseases have limited treatment opportunities, we aimed to mimic the in vivo retinal degenerative process by developing a relevant co-culture system.

METHOD AND MATERIALS: The adult porcine retina was co-cultured with the spontaneously arising human retinal pigment epithelial cells-19 (ARPE-19).

RESULTS: Inflammatory activity was found after culture and included migrating microglial cells, gliosis, cell death, and CS activation (demonstrated by a minor increase in the secreted anaphylotoxin C3a in co-culture). CS components, including C1q, C3, C4, soluble C5b-9, and the C5a receptor, were expressed in the retina and/or ARPE cells after culture. C1q, C3, and CS regulators such as C4 binding protein (C4BP), factor H (CFH), and factor I (CFI) were secreted after culture.

DISCUSSION: Thus, our research indicates that this co-culturing system may be useful for investigations of the CS and its involvement in experimental neurodegenerative diseases.

sted, utgiver, år, opplag, sider
Taylor & Francis, 2018. Vol. 123, nr 1, s. 28-42
Emneord [en]
AMD, RPE, complement system, ocular diseases, retina
HSV kategori
Forskningsprogram
Biomedicinsk vetenskap, Immunologi
Identifikatorer
URN: urn:nbn:se:lnu:diva-71489DOI: 10.1080/03009734.2018.1431744ISI: 000428060300004PubMedID: 29436895Scopus ID: 2-s2.0-85041902488OAI: oai:DiVA.org:lnu-71489DiVA, id: diva2:1189660
Tilgjengelig fra: 2018-03-12 Laget: 2018-03-12 Sist oppdatert: 2019-08-29bibliografisk kontrollert

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Mohlin, CamillaSandholm, KerstinNilsson Ekdahl, Kristina

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