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Myosin 19 is an Outer Mitochondrial Membrane Motor and Effector of Starvation Induced Filopodia with Unique Kinetic Features
Technion, Israel.
Technion, Israel.
Technion, Israel.
2016 (engelsk)Inngår i: Biophysical Journal Supplement 1, 2016, Vol. 110, s. 615a-616a, artikkel-id 3040-PosKonferansepaper, Poster (with or without abstract) (Fagfellevurdert)
Abstract [en]

The interaction between the actin cytoskeleton, myosin motors and their function in mitochondria dynamics, morphology and cellular localization is now beginning to emerge. A novel function for actin-based motors as regulators of cellular adaptations to stress, linking actin cytoskeleton remodelling to mitochondria dynamics. We reveal a novel function for myosin 19 in mitochondrial dynamics and localization during cellular response to glucose starvation. Ectopically expressed myosin 19 localizes with mitochondria at the tips of starvation-induced filopodia. Corollary to this, RNAi mediated knockdown of myosin 19 diminished their formation without evident effects on the mitochondrial network. We analyzed myosin 19 mitochondria interaction and demonstrated that it is uniquely anchored to the outer mitochondrial membrane (OMM) via a 30-residue motif, indicating that myosin 19 is a stably attached OMM molecular motor. To this end, we have purified myosin 19-3IQ motor domain construct. Myosin 19-3IQ featured characteristic actin-activated ATPase activity with moderate to slow turnover (kcat) and relatively tight KATPase. Our transient kinetics and steady state equilibrium binding experiments revealed that myosin 19-3IQ binds ATP and ADP with tight affinity that, to the best of our knowledge, have not yet been exhibited by any other myosins. We suspect that this feature allows myosin 19 to operate in a unique cellular environment that may be related to cellular stress conditions as we showed in our previous studies. The detailed knowledge of myosin 19 enzymatic adaptation will provide us with a quantitative working model of myosin 19, and will assist us to understand its cellular function. Our work reveals a novel function for myosin 19 in mitochondrial positioning during homeostasis and under stress conditions and broadens our understanding of the actin cytoskeleton- myosin -mitochondria interplay.

sted, utgiver, år, opplag, sider
2016. Vol. 110, s. 615a-616a, artikkel-id 3040-Pos
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Forskningsprogram
Kemi, Biokemi
Identifikatorer
URN: urn:nbn:se:lnu:diva-75932DOI: 10.1016/j.bpj.2015.11.3302OAI: oai:DiVA.org:lnu-75932DiVA, id: diva2:1218630
Konferanse
Biophysical Society 60nd Annual Meeting, Feb 27-Mar 02, 2016, Los Angeles
Tilgjengelig fra: 2018-06-14 Laget: 2018-06-14 Sist oppdatert: 2018-06-15bibliografisk kontrollert

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