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Evaluation of Congo Red Staining in Degenerating Porcine Photoreceptors In Vitro: Protective Effects by Structural and Trophic Support
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
Örebro University.
Karolinska Institutet.
Kristianstad University.
2018 (engelsk)Inngår i: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 66, nr 9Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Congo red (CR) is a histological stain used for the detection of extracellular amyloids mediating various neurodegenerative diseases. Given that damaged photoreceptors appear to degenerate similarly to other nerve cells, CR staining was evaluated in experimentally injured porcine retina. CR staining appeared mostly as discrete cytosolic deposits with no obvious plaque formation during the investigated time period. Increases of CR labeling coincided temporally with the known accumulation of mislocalized opsins and increases of cell death. Coculture, either with human retinal pigment epithelium (ARPE) or human neural progenitor (ReN) cells, was accompanied by a significant reduction of CR labeling. Of particular interest was the reduction of CR labeling in cone photoreceptors, which are important for the perception of color and fine details and afflicted in age-related macular degeneration (AMD). Electron microscopy revealed inclusions in the inner segment, cell body, and occasionally synaptic terminals of photoreceptor cells in cultured specimens. Closer examinations indicated the presence of different types of inclusions resembling protein aggregates as well as inclusion bodies. The current results indicate that injury-related response resulted in accumulation of CR deposits in photoreceptor cells, and that trophic and/or structural support attenuated this response.

sted, utgiver, år, opplag, sider
Sage Publications, 2018. Vol. 66, nr 9
Emneord [en]
Congo red, electron microscopy, fluorescence microscopy, photoreceptor, retina
HSV kategori
Forskningsprogram
Kemi, Biokemi
Identifikatorer
URN: urn:nbn:se:lnu:diva-77723DOI: 10.1369/0022155418768222ISI: 000443345700002PubMedID: 29624116Scopus ID: 2-s2.0-85045027011OAI: oai:DiVA.org:lnu-77723DiVA, id: diva2:1248162
Tilgjengelig fra: 2018-09-14 Laget: 2018-09-14 Sist oppdatert: 2019-08-29bibliografisk kontrollert

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