lnu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Extraction and Characterization of Hydrophobin from Trichoderma reesei
Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
2010 (engelsk)Independent thesis Basic level (degree of Bachelor), 10 poäng / 15 hpOppgave
Abstract [en]

Hydrophobins are a class of small proteins (7-15kDa) found in filamentous fungi and are among the most surface active proteins known today. Because of this they have received attention for different applications, e.g. for the food industry as an alternative in emulsions. The goal of this project was to culture and extract hydrophobins from Trichoderma reesei and characterize it. This was done from a freeze-dried culture of Trichoderma reesei, which was cultured on PDA-plates and in liquid medium with glucose as carbon source. Extraction was made by breaking the cells, mechanically and by sonication, and then by shaking a seperating funnel to create foam from the surface-active proteins. The foam was washed and freeze-dried and the total protein concentration of the freeze-dried substance was determined with Bradford assay and the hydrophpbin was characterized with SDS-PAGE. The culturing of the fungi was successful. The amount of foam created was, however, less than expected. The Bradford assay gave a total protein concentration of 7.5% in the freeze-dried substance, but the SDS-PAGE didn't give any results. The reason for this probably depends on the culturing and the extraction of the hydrophobin. T. reesei hydrophobin HFBI, expressed in glucose containing media, is bound to the mycelium of the fungi and the breaking of the mycelium might not have been enough to release all the protein, which also would explain the small amounts of foam. One way to improve this could be to grow the fungi on lactose instead. This will result in that T. reesei produces HFBII instead, which is mainly released to the surrounding. The conclusion of the project is that the method for culturing and extraction needs to be improved to obtain hydrophobin from T. reesei.

sted, utgiver, år, opplag, sider
2010. , s. 30
Emneord [en]
hydrophobins, fungi
HSV kategori
Identifikatorer
URN: urn:nbn:se:lnu:diva-8432OAI: oai:DiVA.org:lnu-8432DiVA, id: diva2:351716
Uppsök
Physics, Chemistry, Mathematics
Veileder
Examiner
Tilgjengelig fra: 2010-09-17 Laget: 2010-09-15 Sist oppdatert: 2018-01-12bibliografisk kontrollert

Open Access i DiVA

fulltekst(10351 kB)8675 nedlastinger
Filinformasjon
Fil FULLTEXT02.pdfFilstørrelse 10351 kBChecksum SHA-512
7b834bf0cd12e1c24d50cae64d93ef130c4abade21d575fc63d6007a9e5c5651585f992b985cc69cb1f7d1768206199500cd30f5579648728c22f603684fc894
Type fulltextMimetype application/pdf

Søk i DiVA

Av forfatter/redaktør
Johansson, Helene
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 8675 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

urn-nbn

Altmetric

urn-nbn
Totalt: 352 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf