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Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB). (Linnaeus Ctr Biomat Chem, BMC)
Lund university.
Lund university.
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB). (Linnaeus Ctr Biomat Chem, BMC)
Vise andre og tillknytning
2013 (engelsk)Inngår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 436, nr 3, s. 551-556Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na+/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate. 

sted, utgiver, år, opplag, sider
Elsevier , 2013. Vol. 436, nr 3, s. 551-556
Emneord [en]
Pho89, Pichia pastoris, Oligomer, Reconstitution, Phosphate transport, Circular dichroism
HSV kategori
Forskningsprogram
Kemi, Biokemi
Identifikatorer
URN: urn:nbn:se:lnu:diva-27720DOI: 10.1016/j.bbrc.2013.06.011ISI: 000321995900034Scopus ID: 2-s2.0-84879889835OAI: oai:DiVA.org:lnu-27720DiVA, id: diva2:638664
Prosjekter
Characterization of sensors and signal transduction in regulation of phosphate uptake systems
Forskningsfinansiär
Swedish Research Council, 621-2007-6144Swedish Research Council, 522-2008-3724,7480Tilgjengelig fra: 2013-08-01 Laget: 2013-08-01 Sist oppdatert: 2018-11-16bibliografisk kontrollert

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Sengottaiyan, PalaniveluRuiz-Pavon, LorenaPersson, Bengt L.

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