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Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
Linköping Univ, Sweden.
Linköping Univ, Sweden.
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB).
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2014 (engelsk)Inngår i: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 461, s. 57-59Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In this study, we compared affinity data from surface plasmon resonance (SPR) and weak affinity chromatography (WAC), two established techniques for determination of weak affinity (mM-mu M) small molecule-protein interactions. In the current comparison, thrombin was used as target protein. In WAC the affinity constant (K-D) was determined from retention times, and in SPR it was determined by Langmuir isotherm fitting of steady-state responses. Results indicate a strong correlation between the two methods (R-2 = 0.995, P < 0.0001). (C) 2014 Elsevier Inc. All rights reserved.

sted, utgiver, år, opplag, sider
2014. Vol. 461, s. 57-59
Emneord [en]
Affinity determination, Fragment-based drug discovery, Surface plasmon resonance, Thrombin, Weak affinity chromatography
HSV kategori
Forskningsprogram
Kemi, Biokemi
Identifikatorer
URN: urn:nbn:se:lnu:diva-36862DOI: 10.1016/j.ab.2014.05.023ISI: 000340077600009Scopus ID: 2-s2.0-84903973479OAI: oai:DiVA.org:lnu-36862DiVA, id: diva2:745576
Tilgjengelig fra: 2014-09-10 Laget: 2014-09-10 Sist oppdatert: 2017-12-05bibliografisk kontrollert

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Duong-Thi, Minh-DaoBergström, MariaOhlson, Sten

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