lnu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Extreme arsenic resistance by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1.
University of East Anglia, UK ; University of Georgia, USA.
University of East Anglia, UK ; Umeå University.ORCID-id: 0000-0002-9622-3318
University of East Anglia, UK.
John Innes Centre, Norwich, UK.
Vise andre og tillknytning
2007 (engelsk)Inngår i: Extremophiles, ISSN 1431-0651, E-ISSN 1433-4909, Vol. 11, nr 3, s. 425-34Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

'Ferroplasma acidarmanus' Fer1 is an arsenic-hypertolerant acidophilic archaeon isolated from the Iron Mountain mine, California; a site characterized by heavy metals contamination. The presence of up to 10 g arsenate per litre [As(V); 133 mM] did not significantly reduce growth yields, whereas between 5 and 10 g arsenite per litre [As(III); 67-133 mM] significantly reduced the yield. Previous bioinformatic analysis indicates that 'F. acidarmanus' Fer1 has only two predicted genes involved in arsenic resistance and lacks a recognizable gene for an arsenate reductase. Biochemical analysis suggests that 'F. acidarmanus' Fer1 does not reduce arsenate indicating that 'F. acidarmanus' Fer1 has an alternative resistance mechanism to arsenate other than reduction to arsenite and efflux. Primer extension analysis of the putative ars transcriptional regulator (arsR) and efflux pump (arsB) demonstrated that these genes are co-transcribed, and expressed in response to arsenite, but not arsenate. Two-dimensional polyacrylamide gel electrophoresis analysis of 'F. acidarmanus' Fer1 cells exposed to arsenite revealed enhanced expression of proteins associated with protein refolding, including the thermosome Group II HSP60 family chaperonin and HSP70 DnaK type heat shock proteins. This report represents the first molecular and proteomic study of arsenic resistance in an acidophilic archaeon.

sted, utgiver, år, opplag, sider
2007. Vol. 11, nr 3, s. 425-34
HSV kategori
Forskningsprogram
Naturvetenskap, Mikrobiologi
Identifikatorer
URN: urn:nbn:se:lnu:diva-37330DOI: 10.1007/s00792-006-0052-zPubMedID: 17268768OAI: oai:DiVA.org:lnu-37330DiVA, id: diva2:750364
Tilgjengelig fra: 2014-09-29 Laget: 2014-09-29 Sist oppdatert: 2017-12-05bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Dopson, Mark

Søk i DiVA

Av forfatter/redaktør
Dopson, Mark
I samme tidsskrift
Extremophiles

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 145 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf