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Cell Adhesion Induced Using Surface Modification with Cell-Penetrating Peptide-Conjugated Poly(ethylene glycol)-Lipid: A New Cell Glue for 3D Cell-Based Structures
University of Tokyo, Japan;Uppsala University.
Uppsala University.
Linnéuniversitetet, Fakulteten för Hälso- och livsvetenskap (FHL), Institutionen för kemi och biomedicin (KOB). Uppsala University. (Linnaeus Ctr Biomat Chem, BMC)ORCID-id: 0000-0001-7888-1571
Uppsala University Hospital.
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2017 (Engelska)Ingår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 9, nr 1, s. 244-254Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

We synthesized a novel material, cell-penetrating peptide conjugated poly(ethylene glycol)-lipid (CPP-PEG-lipid), that can induce the adhesion of floating cells. Firm cell adhesion with spreading could be induced by cell surface modification with the CPP-PEG-lipids. Cell adhesion was induced by CPPs but not by any other cationic short peptides we tested. Here, we demonstrated adherence using the floating cell line CCRF-CEM as well as primary human T cells, B cells, erythrocytes, and hepatocytes. As compared to cells grown in suspension, adherent cells were more rapidly induced to attach to substrates with the cell-surface modification. The critical factor for attachment was localization of CPPs at the cell membrane by PEG-lipids with PEG > 20 kDa. These cationic CPPs on PEG chains were able to interact with substrate surfaces such as polystyrene (PS) surfaces, glass surfaces, and PS microfibers that are negatively charged, inducing firm cell adhesion and cell spreading. Also, as opposed to normal cationic peptides that interact strongly with cell membranes, CPPs were less interactive with the cell surfaces because of their cell-penetrating property, making them more available for adhering cells to the substrate surface. No effects on cell viability or cell proliferation were observed after the induction of cell adhesion. With this technique, cells could be easily immobilized onto PS microfibers, an important step in fabricating 3D cell-based structures. Cells immobilized onto 3D PS microfibers were alive, and human hepatocytes showed normal production of urea and albumin on the microfibers. This method is novel in inducing firm cell adhesion-via a one-step treatment.

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2017. Vol. 9, nr 1, s. 244-254
Nyckelord [en]
cell surface modification, poly(ethylene glycol)-conjugated phospholipid (PEG-lipid), cell-penetrating peptide (CPP), cell adhesion, 3D structure
Nationell ämneskategori
Biokemi och molekylärbiologi
Forskningsämne
Naturvetenskap, Biomedicinsk vetenskap
Identifikatorer
URN: urn:nbn:se:lnu:diva-60801DOI: 10.1021/acsami.6b14584ISI: 000392037400031OAI: oai:DiVA.org:lnu-60801DiVA, id: diva2:1075987
Tillgänglig från: 2017-02-21 Skapad: 2017-02-21 Senast uppdaterad: 2019-09-05Bibliografiskt granskad

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Nilsson Ekdahl, Kristina

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ACS Applied Materials and Interfaces
Biokemi och molekylärbiologi

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