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QCM sensing of multivalent interactions between lectins and well-defined glycosylated nanoplatforms
Univ Namur, Belgium;PSL Univ, France.
KTH Royal Instute of Technology, Sweden.
Attana AB, Sweden.
Attana AB, Sweden.
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2019 (Engelska)Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 139, artikel-id 111328Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Quartz crystal microbalance (QCM) methodology has been adopted to unravel important factors contributing to the "cluster glycoside effect" observed in carbohydrate-lectin interactions. Well-defined, glycosylated nanostructures of precise sizes, geometries and functionalization patterns were designed and synthesized, and applied to analysis of the interaction kinetics and thermodynamics with immobilized lectins. The nanostructures were based on Borromean rings, dodecaamine cages, and fullerenes, each of which carrying a defined number of carbohydrate ligands at precise locations. The synthesis of the Borromeates and dodecaamine cages was easily adjustable due to the modular assembly of the structures, resulting in variations in presentation mode. The binding properties of the glycosylated nanoplatforms were evaluated using flow-through QCM technology, as well as hemagglutination inhibition assays, and compared with dodecaglycosylated fullerenes and a monovalent reference. With the QCM setup, the association and dissociation rate constants and the associated equilibrium constants of the interactions could be estimated, and the results used to delineate the multivalency effects of the lectin-nanostructure interactions.

Ort, förlag, år, upplaga, sidor
Elsevier, 2019. Vol. 139, artikel-id 111328
Nyckelord [en]
QCM, Carbohydrates, Lectins, Multivalency, Nanoplatforms
Nationell ämneskategori
Biokemi och molekylärbiologi
Forskningsämne
Naturvetenskap, Kemi
Identifikatorer
URN: urn:nbn:se:lnu:diva-86911DOI: 10.1016/j.bios.2019.111328ISI: 000472696000013PubMedID: 31136921Scopus ID: 2-s2.0-85066129421OAI: oai:DiVA.org:lnu-86911DiVA, id: diva2:1337946
Tillgänglig från: 2019-07-18 Skapad: 2019-07-18 Senast uppdaterad: 2020-05-13Bibliografiskt granskad

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Ramström, Olof

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Institutionen för kemi och biomedicin (KOB)
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Totalt: 193 träffar
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