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High-Moisture Meat Analogues Produced from Yellow Pea and Faba Bean Protein Isolates/Concentrate: Effect of Raw Material Composition and Extrusion Parameters on Texture Properties
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Linnaeus University, Linnaeus Knowledge Environments, Sustainable Health. (Food science and nutrition)ORCID iD: 0000-0001-6973-4190
Lund University, Sweden.ORCID iD: 0000-0002-0622-7156
Chalmers University, Sweden.ORCID iD: 0000-0002-5317-2768
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Linnaeus University, Linnaeus Knowledge Environments, Sustainable Health. Mansoura University, Egypt. (Food science and nutrition)ORCID iD: 0000-0003-0550-5828
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2021 (English)In: Foods, E-ISSN 2304-8158, Vol. 10, no 4, article id 843Article in journal (Refereed) Published
Sustainable development
SDG 2: End hunger, achieve food security and improved nutrition, and promote sustainable agriculture, SDG 12: Ensure sustainable consumption and production patterns
Abstract [en]

Yellow pea and faba bean are potential candidates to replace soybean-based ingredients due to their suitability for cultivation in the northern hemisphere, non-genetically modified organisms cultivation practice and low risk of allergenicity. This study examined the functionality of local yellow pea and faba bean protein isolates/concentrate as meat analogue products. The most critical factors affecting the texture properties of meat analogue were also determined. Extrusion was used to produce high-moisture meat analogues (HMMAs) from yellow pea and faba bean protein isolates/concentrates and HMMAs with fibrous layered structures was successfully produced from both imported commercial and local sources. The texture properties of the HMMA produced were mainly affected by the ash, fiber and protein content and water-holding capacity of the source protein. Three extrusion process parameters (target moisture content, extrusion temperature, screw speed), also significantly affected HMMA texture. In conclusion, functional HMMA can be produced using protein isolates derived from locally grown pulses.

Place, publisher, year, edition, pages
Switzerland: MDPI, 2021. Vol. 10, no 4, article id 843
Keywords [en]
high-moisture extrusion (HME); plant protein; meat analogue; yellow pea; faba bean; pulses
National Category
Food Science
Research subject
Natural Science, Food Science
Identifiers
URN: urn:nbn:se:lnu:diva-102137DOI: 10.3390/foods10040843ISI: 000643046700001PubMedID: 33924424Scopus ID: 2-s2.0-85104127502Local ID: 2021OAI: oai:DiVA.org:lnu-102137DiVA, id: diva2:1543907
Funder
Swedish Research Council Formas, 942-2016-38Available from: 2021-04-13 Created: 2021-04-13 Last updated: 2023-04-20Bibliographically approved
In thesis
1. The development of novel foods from Swedish pulses: Raw material composition and processing effects
Open this publication in new window or tab >>The development of novel foods from Swedish pulses: Raw material composition and processing effects
2021 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

A gradual shift towards a healthier and more sustainable diet with a higher quantity of plant-based products is suggested to be one of the most efficient ways to alleviate environmental pressure from the current food system. Pulses could play a crucial role in this shift due to the multi-faceted benefits they have on the environment and human health. This thesis aims to study the suitability of Swedish grown pulses as the raw material for new pulse-based foods and ingredients. 

Flour from locally grown pulses (yellow peas, grey peas, faba beans, and white beans) prepared using different treatments (boiling, roasting, and germination) were analysed for their functional properties, nutrient content, and volatile compound composition. Protein isolates from locally grown pulses were prepared at a pilot scale using an alkaline extraction method, followed by isoelectric precipitation and were then analysed for proximate composition, thermal properties, and water holding capacity. The suitability of pulse flour in the development of cheese analogues and pulse protein isolates to produce high-moisture meat analogues (HMMA) was examined. 

The results showed that the functional properties and nutrient content of flour from pulses were significantly affected by treatments prior to milling. Different treatments also affected the volatile compound profile of pulse flour to varying degrees. Plant-based cheese analogues with a firm and sliceable texture were successfully prepared using flour from boiled and roasted yellow peas and faba beans. The pulse-based cheese analogues could be categorised as functional foods owing to the high content of dietary fibre (7-8 g/100 g). Moreover, meat analogues can be produced using protein isolates from yellow peas and faba beans using high-moisture extrusion cooking. The target moisture content and extrusion temperature needed to be adjusted depending on the botanical origin of the protein isolate. Pulse-based cheese and meat analogues made from locally sourced materials could be utilised to increase the consumption of pulses in Sweden, which is currently very low.  

Abstract [sv]

En gradvis övergång till en mer hälsosam och hållbar kost med en högre andel växtbaserade produkter föreslås vara ett av de mest effektiva sätten att minska klimatpåverkan från konsumtion av livsmedel. Baljväxter kan spela en viktig roll i detta skifte på grund av deras fördelar för både miljön och hälsan. Den här avhandlingen har målsättningen att studera lämpligheten hos baljväxter odlade i Sverige som råvara för nya växtbaserade livsmedel och ingredienser. 

Mjöl från lokalt odlade baljväxter (gula ärtor, gråärtor, bondbönor, och vita bönor) behandlade med olika beredning metoder (kokning, rostning och groning) analyserades med avseende på funktionella egenskaper, näringsinnehåll och flyktiga ämnen. Proteinisolat från lokalt odlade baljväxter togs fram i pilotskala med en alkalisk extraktionsmetod följt av isoelektrisk utfällning och analyserades för sammansättning av makronutrienter, termiska egenskaper och vattenhållningskapacitet. Vidare undersöktes lämpligheten av baljväxtmjöl i utvecklingen av växtbaserade ostar och lämpligheten av proteinisolat från baljväxter för att producera köttsubstitut. 

Resultaten visade att baljväxtmjölets funktionella egenskaper och näringsinnehåll påverkades avsevärt av kokning, rostning och groning. Dessa behandlingar påverkade också profilen av flyktiga ämnen. Växtbaserade ostanaloger med fast och skivbar konsistens framställdes med mjöl från kokade och rostade gula ärtor och bondbönor. De växtbaserade ostarna kan kategoriseras som funktionella livsmedel på grund av det höga innehållet av kostfibrer. Dessutom kunde köttsubstitut produceras med proteinisolat från gula ärtor och bondbönor genom våt-extrudering. Vattenhalten och extruderingstemperaturen behövde justeras beroende på råvaran i ingående proteinisolat. Växtbaserade ost- och köttsubstitut tillverkade av lokala råvaror skulle kunna användas för att öka baljväxterskonsumtionen i Sverige, som för närvarande är mycket låg.  

Place, publisher, year, edition, pages
Sweden: Linnaeus University Press, 2021. p. 66
Series
Linnaeus University Dissertations
Keywords
legumes, pulse flour, functional properties, dietary fibre, folate, volatile compounds, cheese analogue, protein isolate, high-moisture extrusion, meat analogue.
National Category
Food Science
Research subject
Natural Science, Food Science
Identifiers
urn:nbn:se:lnu:diva-106842 (URN)9789189460058 (ISBN)9789189460065 (ISBN)
Public defence
2021-10-08, Vi1158 K Lapis, Linnaeus University, Kalmar, 09:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council Formas, 942-2016-38
Available from: 2021-09-14 Created: 2021-09-14 Last updated: 2024-03-04Bibliographically approved

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Ferawati, FerawatiHefni, Mohammed E.Witthöft, Cornelia M.

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