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Functional expression of µ-opioid receptors in the human colon cancer cell line, HT-29, and their localization in human colon.
University of Kalmar, School of Pure and Applied Natural Sciences. Department of Surgery, Sahlgrenska University Hospital, Göteborg, SE-413 45, Sweden.
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2008 (English)In: Digestive Diseases and Sciences, ISSN 0163-2116, E-ISSN 1573-2568, Vol. 53, no 2, p. 461-466Article in journal (Refereed) Published
Abstract [en]

We have investigated the functional expression of μ-opioid receptors (MORs) in the human colon cancer cell line, HT-29. As revealed by immunocytochemistry, immunoreactivity was present in both the cytoplasm and nuclei of the cells. Challenge with morphine for 24 h (1 nM to 1 μM) barely affected cell proliferation, while the secretion of urokinase type plasminogen activator (a protease involved in invasion/metastasis) was markedly augmented by a concentration of 0.1 μM. Human colon cancer tissue from 14 consecutively operated patients was investigated by immunohistochemistry. MORs were found in the nuclei of colonocytes and immune cells of the lamina propria in tumor-free tissue. In tumor tissue, immunoreactivity was found in the membrane and often in the nuclei of tumor cells. The current findings suggest that morphine administration could affect tumor progression by interfering with, for example, invasive properties. Our demonstration of a nuclear expression of the MORs appears to be a novel finding.

Place, publisher, year, edition, pages
2008. Vol. 53, no 2, p. 461-466
National Category
Medical and Health Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:hik:diva-2321DOI: 10.1007/s10620-007-9897-yOAI: oai:DiVA.org:hik-2321DiVA, id: diva2:274883
Available from: 2009-11-03 Created: 2009-11-02 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Aspects of Non-Neuronal Signalling Functions of Acetylcholine in Colorectal Cancer: Roles for the α7nAChR
Open this publication in new window or tab >>Aspects of Non-Neuronal Signalling Functions of Acetylcholine in Colorectal Cancer: Roles for the α7nAChR
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Kalmar: Högskolan i Kalmar, 2009. p. 60
Series
Dissertation series / University of Kalmar, Faculty of Natural Science, ISSN 1650-2779 ; 76
Keywords
alpha7 nicotinic acetylcholine receptor, acetylcholine, colorectal cancer, morphine, nicotine, plasminogen activator inhibitor-1, SLURP-1
National Category
Cancer and Oncology
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:hik:diva-2348 (URN)978-91-85993-37-6 (ISBN)
Public defence
2009-09-25, Falkens hörsal (C305), Nygatan 18B, Kalmar, 09:00 (English)
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Available from: 2009-11-04 Created: 2009-11-04 Last updated: 2016-03-11Bibliographically approved

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Pettersson, AnnDelbro, Dick

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