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A Procedure for the Determination of Optimal Chitosan Concentrations for Elicitation of Cultured Plant Cells.
Institute of Biotechnology ETH-Hönggerberg CH-8093 Zürich, Switzerland.ORCID iD: 0000-0001-8899-5046
1989 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 28, no 10, p. 2651-2654Article in journal (Refereed) Published
Abstract [en]

An experimental method for determination of the optimum chitosan concentration for elicitation of plant cell suspension cultures is presented. The procedure, which is based on measurements of the conductivity of the culture medium after addition of chitosan, has been applied to suspension cultures of Nicotiana tabacum and Eschscholtzia californica. Increased conductivity of the medium (due to permeabilization of the cells) results in decreased secondary product formation and cell growth. Maximum product formation is observed for cells elicited with the highest chitosan concentration which does not affect membrane permeability. 

Place, publisher, year, edition, pages
1989. Vol. 28, no 10, p. 2651-2654
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biochemistry
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URN: urn:nbn:se:lnu:diva-605DOI: 10.1016/S0031-9422(00)98060-9OAI: oai:DiVA.org:lnu-605DiVA, id: diva2:307335
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2017-12-12Bibliographically approved

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Brodelius, Peter

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