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The Synthesis of 8-(6-Aminohexyl)-Amino-GMP and Its Applications as a General Ligand in Affinity Chromatography
UNIV LUND, CTR CHEM, DEPT PURE & APPL BIOCHEM, S-22007 LUND 7, SWEDEN .ORCID iD: 0000-0001-8899-5046
1978 (English)In: Archives of Biochemistry and Biophysics, Vol. 188, no 1, p. 228-231Article in journal (Refereed) Published
Abstract [en]

The synthesis of a new general ligand, i.e., 8-(6-aminohexyl)-amino-GMP, has been achieved by bromination of GMP and subsequent substitution of the bromine for hexamethylene diamine. The overall yield of the synthesis has been 60 to 70%. This new general ligand was immobilized on BrN-activated Sepharose and used as an affinity adsorbent for inosinic acid dehydrogenase (E.C. 1.2.1.14) from Aerobacter aerogenes. Various elution methods were investigated in order to increase the specific activity of the purified enzyme. 

Place, publisher, year, edition, pages
1978. Vol. 188, no 1, p. 228-231
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-614DOI: 10.1016/0003-9861(78)90377-6OAI: oai:DiVA.org:lnu-614DiVA, id: diva2:307344
Available from: 2010-04-01 Created: 2010-04-01 Last updated: 2016-10-25Bibliographically approved

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Brodelius, Peter

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