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A simple method for long-term storage Acanthamoeba species
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.
University of Kalmar, School of Pure and Applied Natural Sciences.ORCID iD: 0000-0002-1152-4235
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2009 (English)In: Parasitology Research, ISSN 0932-0113, E-ISSN 1432-1955, Vol. 104, no 4, p. 935-937Article in journal (Refereed) Published
Abstract [en]

We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyphaga and 3 years for Acanthamoeba castellanii and Acanthamoeba rhysodes. Advantages of this storage method are: (1) it is quick and simple, (2) inexpensive, (3) does not require encystment before storage, (4) resuscitation of cysts can be achieved within a week of culture in PYG medium at 27A degrees C, and does not require co-culture with bacteria or any special equipment.

Place, publisher, year, edition, pages
2009. Vol. 104, no 4, p. 935-937
National Category
Microbiology
Research subject
Ecology, Zoonotic Ecology; Ecology, Microbiology; Biomedical Sciences, Virology
Identifiers
URN: urn:nbn:se:lnu:diva-1912DOI: 10.1007/s00436-008-1304-xOAI: oai:DiVA.org:lnu-1912DiVA, id: diva2:308960
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2020-01-28Bibliographically approved

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Axelsson Olsson, DianaOlofsson, JennyEllström, PatrikWaldenström, JonasOlsen, Björn

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