lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Studies of Echovirus 5 interactions with the cell surface: Heparan sulfate mediates attachment to the host cell.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
Show others and affiliations
2010 (English)In: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 151, no 2, p. 170-176Article in journal (Refereed) Published
Abstract [en]

Infections caused by Echovirus 5 (E5), an enterovirus of the Picornaviridae family, have been associated with fever, rashes and sporadic cases of aseptic meningitis. To elucidate the receptor usage of this virus, the significance of a previously proposed integrin binding arginine-glycine-aspartic acid (RGD) motif found in the VP3 capsid protein was investigated, as well as the capacity of E5 to interact with heparan sulfate on the cell surface. Using the prototype strain E5 Noyce (E5N), an E5N mutant where the aspartic acid of the RGD motif has been substituted to a glutamic acid and clinical E5 isolates, the RGD motif of VP3 was found to be non-essential and hence not involved in integrin receptor binding. However, E5N and clinical E5 isolates interact with heparan sulfate at the cell surface, as demonstrated by virus replication inhibition assays using heparin and heparinase III, and studies of E5 interactions at the cell surface measured by real-time PCR analysis. In conclusion, E5 utilizes heparan sulfate as a cellular receptor, but the RGD motif of VP3 is not essential for E5 infectivity.

Place, publisher, year, edition, pages
2010. Vol. 151, no 2, p. 170-176
National Category
Microbiology in the medical area
Research subject
Biomedical Sciences, Virology
Identifiers
URN: urn:nbn:se:lnu:diva-7169DOI: 10.1016/j.virusres.2010.05.001PubMedID: 20466025Scopus ID: 2-s2.0-77954219093OAI: oai:DiVA.org:lnu-7169DiVA, id: diva2:343346
Available from: 2010-08-13 Created: 2010-08-13 Last updated: 2018-01-12Bibliographically approved
In thesis
1. Tissue tropism and oncolytic potential of enteroviruses
Open this publication in new window or tab >>Tissue tropism and oncolytic potential of enteroviruses
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Vävnadstropism och onkolytisk potential hos enterovirus
Place, publisher, year, edition, pages
Växjö, Kalmar: Linnaeus University Press, 2012
Series
Linnaeus University Dissertations ; 77/2012
Keywords
picornavirus, enterovirus, echovirus, receptor, tropism, viral oncolysis, quantification, receptor interactions, colon cancer
National Category
Other Natural Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
urn:nbn:se:lnu:diva-17721 (URN)978-91-86983-32-1 (ISBN)
Public defence
2012-03-09, N2007, Smålandsgatan 26B, Kalmar, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2012-02-28 Created: 2012-02-22 Last updated: 2012-02-28Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Israelsson, StinaGullberg, MariaJonsson, NinaEdman, KjellLindberg, A. Michael

Search in DiVA

By author/editor
Israelsson, StinaGullberg, MariaJonsson, NinaEdman, KjellLindberg, A. Michael
By organisation
School of Natural Sciences
In the same journal
Virus Research
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 214 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf