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Development of a fluorometric method for the quantification of sulfite and thiol-containing compounds in beer
Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
2011 (engelsk)Independent thesis Advanced level (degree of Master (One Year)), 20 poäng / 30 hpOppgave
Abstract [en]

Sulfite is the most important antioxidant in beer. Quantification of sulfite is crucial due to restrictions as an additive and for investigative purposes of sulfite and thiol-containing proteins role as redox mediators. A method based on fluorometric determination of sulfite and thiol-containing compounds in beer was developed. The analytes were derivatized with ThioGlo®  1 and subsequently separated on a C18 column with wide pore size, featuring a high-performance liquid chromatography system equipped with fluorescence detector. Two peaks corresponding to sulfite derivatives were observed. The two derivatives were assessed with exact mass spectrometry and both provided identical mass spectra. To compensate for adverse matrix effects in samples a matrix-matched calibration curve is proposed. Sulfite diminished in an inverse exponential manner upon hydrogen peroxide addition in beer. The amount of thiol groups decreased when beer was subjected to oxidative stress, thus confirming its antioxidative role in beer.

sted, utgiver, år, opplag, sider
2011. , s. 49
Emneord [en]
sulfite, beer analysis, matrix match, ThioGlo® 1, thiol-containing compounds, oxidative stress, liquid chromatography, fluorescence
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Identifikatorer
URN: urn:nbn:se:lnu:diva-10909OAI: oai:DiVA.org:lnu-10909DiVA, id: diva2:399662
Uppsök
Physics, Chemistry, Mathematics
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Examiner
Tilgjengelig fra: 2011-02-23 Laget: 2011-02-23 Sist oppdatert: 2011-02-23bibliografisk kontrollert

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