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Development of a fluorometric method for the quantification of sulfite and thiol-containing compounds in beer
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
2011 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Sulfite is the most important antioxidant in beer. Quantification of sulfite is crucial due to restrictions as an additive and for investigative purposes of sulfite and thiol-containing proteins role as redox mediators. A method based on fluorometric determination of sulfite and thiol-containing compounds in beer was developed. The analytes were derivatized with ThioGlo®  1 and subsequently separated on a C18 column with wide pore size, featuring a high-performance liquid chromatography system equipped with fluorescence detector. Two peaks corresponding to sulfite derivatives were observed. The two derivatives were assessed with exact mass spectrometry and both provided identical mass spectra. To compensate for adverse matrix effects in samples a matrix-matched calibration curve is proposed. Sulfite diminished in an inverse exponential manner upon hydrogen peroxide addition in beer. The amount of thiol groups decreased when beer was subjected to oxidative stress, thus confirming its antioxidative role in beer.

Place, publisher, year, edition, pages
2011. , p. 49
Keywords [en]
sulfite, beer analysis, matrix match, ThioGlo® 1, thiol-containing compounds, oxidative stress, liquid chromatography, fluorescence
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:lnu:diva-10909OAI: oai:DiVA.org:lnu-10909DiVA, id: diva2:399662
Uppsok
Physics, Chemistry, Mathematics
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Available from: 2011-02-23 Created: 2011-02-23 Last updated: 2011-02-23Bibliographically approved

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