lnu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Tissue tropism and oncolytic potential of enteroviruses
Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
2012 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)Alternativ titel
Vävnadstropism och onkolytisk potential hos enterovirus (Svenska)
Ort, förlag, år, upplaga, sidor
Växjö, Kalmar: Linnaeus University Press , 2012.
Serie
Linnaeus University Dissertations ; 77/2012
Nyckelord [en]
picornavirus, enterovirus, echovirus, receptor, tropism, viral oncolysis, quantification, receptor interactions, colon cancer
Nationell ämneskategori
Annan naturvetenskap
Forskningsämne
Naturvetenskap, Biomedicinsk vetenskap
Identifikatorer
URN: urn:nbn:se:lnu:diva-17721ISBN: 978-91-86983-32-1 (tryckt)OAI: oai:DiVA.org:lnu-17721DiVA, id: diva2:504897
Disputation
2012-03-09, N2007, Smålandsgatan 26B, Kalmar, 09:00 (Svenska)
Opponent
Handledare
Tillgänglig från: 2012-02-28 Skapad: 2012-02-22 Senast uppdaterad: 2012-02-28Bibliografiskt granskad
Delarbeten
1. Significance of an authentic 5´ genomic end for activation of viral replication using in vitro transcripts of echovirus 5 and its implication for the efficacy of oncolytic infectious nucleic acid
Öppna denna publikation i ny flik eller fönster >>Significance of an authentic 5´ genomic end for activation of viral replication using in vitro transcripts of echovirus 5 and its implication for the efficacy of oncolytic infectious nucleic acid
Visa övriga...
(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Nationell ämneskategori
Annan medicin och hälsovetenskap Annan naturvetenskap
Forskningsämne
Naturvetenskap, Biomedicinsk vetenskap
Identifikatorer
urn:nbn:se:lnu:diva-17720 (URN)
Tillgänglig från: 2012-02-22 Skapad: 2012-02-22 Senast uppdaterad: 2016-11-18Bibliografiskt granskad
2. A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR
Öppna denna publikation i ny flik eller fönster >>A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR
2009 (Engelska)Ingår i: Virology Journal, ISSN 1743-422X, E-ISSN 1743-422X, Vol. 6, nr Article ID: 217Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Measuring virus attachment to host cells is of great importance when trying to identify novel receptors. The presence of a usable receptor is a major determinant of viral host range and cell tropism. Furthermore, identification of appropriate receptors is central for the understanding of viral pathogenesis and gives possibilities to develop antiviral drugs. Attachment is presently measured using radiolabeled and subsequently gradient purified viruses. Traditional methods are expensive and time-consuming and not all viruses are stable during a purification procedure; hence there is room for improvement. Real-time PCR (RT-PCR) has become the standard method to detect and quantify virus infections, including enteroviruses, in clinical samples. For instance, primers directed to the highly conserved 5' untranslated region (5'UTR) of the enterovirus genome enable detection of a wide spectrum of enteroviruses. Here, we evaluate the capacity of the RT-PCR technology to study enterovirus host cell interactions at the cell surface and compare this novel implementation with an established assay using radiolabeled viruses. Results: Both purified and crude viral extracts of CVB5 generated comparable results in attachment studies when analyzed with RT-PCR. In addition, receptor binding studies regarding viruses with coxsackie- nd adenovirus receptor (CAR) and/or decay accelerating factor (DAF) affinity, further demonstrated the possibility to use RT-PCR to measure virus attachment to host cells. Furthermore, the RT-PCR technology and crude viral extracts was used to study attachment with low multiplicity of infection (0.05 x 10(-4)TCID(50)/cell) and low cell numbers (250), which implies the range of potential implementations of the presented technique. Conclusion: We have implemented the well-established RT-PCR technique to measure viral attachment to host cells with high accuracy and reproducibility, at low cost and with less effort than traditional methods. Furthermore, replacing traditional methods with RT-PCR offers the opportunity to use crude virus containing extracts to investigate attachment, which could be considered as a step towards viral attachment studies in a more natural state.

Nationell ämneskategori
Mikrobiologi
Forskningsämne
Biomedicinsk vetenskap, Virologi; Naturvetenskap, Mikrobiologi; Naturvetenskap, Biomedicinsk vetenskap
Identifikatorer
urn:nbn:se:lnu:diva-2142 (URN)10.1186/1743-422X-6-217 (DOI)000273070600001 ()
Tillgänglig från: 2010-04-06 Skapad: 2010-04-06 Senast uppdaterad: 2017-12-12Bibliografiskt granskad
3. A novel and rapid method to quantify cytolytic replication of picornaviruses in cell culture
Öppna denna publikation i ny flik eller fönster >>A novel and rapid method to quantify cytolytic replication of picornaviruses in cell culture
2005 (Engelska)Ingår i: Journal of virological methods, Vol. 130, s. 117-123Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Determining viral titers is a key issue in a wide variety of studies regarding different aspects of virology. The standard methods used for determining picornavirus titers are endpoint titration assay and plaque assay, both time consuming and laborious. The method described uses the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide) that is reduced to formazane by cellular dehydrogenase, genes shown to be down-regulated during picornavirus infection. The amount formazane produced correlates with the viral titers obtained and can easily be measured using an ELISA plate reader. The colorimetric method has been evaluated using virus types from different genera of the Picornaviridae family. The MTT method reduces the time spent on determining the viral titers and still maintains a reliable accuracy.

Nationell ämneskategori
Mikrobiologi inom det medicinska området
Forskningsämne
Biomedicinsk vetenskap, Virologi; Naturvetenskap, Biomedicinsk vetenskap; Naturvetenskap, Mikrobiologi
Identifikatorer
urn:nbn:se:lnu:diva-564 (URN)10.1016/j.jviromet.2005.06.016 (DOI)
Tillgänglig från: 2010-04-01 Skapad: 2010-04-01 Senast uppdaterad: 2018-01-12Bibliografiskt granskad
4. Cytolytic replication of echoviruses in colon cancer cell lines
Öppna denna publikation i ny flik eller fönster >>Cytolytic replication of echoviruses in colon cancer cell lines
2011 (Engelska)Ingår i: Virology Journal, ISSN 1743-422X, E-ISSN 1743-422X, Vol. 8, artikel-id e473Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

BACKGROUND: Colorectal cancer is one of the most common cancers in the world, killing nearly 50% of patients afflicted. Though progress is being made within surgery and other complementary treatments, there is still need for new and more effective treatments. Oncolytic virotherapy, meaning that a cancer is cured by viral infection, is a promising field for finding new and improved treatments. We have investigated the oncolytic potential of several low-pathogenic echoviruses with rare clinical occurrence. Echoviruses are members of the enterovirus genus within the family Picornaviridae.

METHODS: Six colon cancer cell lines (CaCo-2, HT29, LoVo, SW480, SW620 and T84) were infected by the human enterovirus B species echovirus 12, 15, 17, 26 and 29, and cytopathic effects as well as viral replication efficacy were investigated. Infectivity was also tested in spheroids grown from HT29 cells.

RESULTS: Echovirus 12, 17, 26 and 29 replicated efficiently in almost all cell lines and were considered highly cytolytic. The infectivity of these four viruses was further evaluated in artificial tumors (spheroids), where it was found that echovirus 12, 17 and 26 easily infected the spheroids.

CONCLUSIONS: We have found that echovirus 12, 17 and 26 have potential as oncolytic agents against colon cancer, by comparing the cytolytic capacity of five low-pathogenic echoviruses in six colon cancer cell lines and in artificial tumors.

Nationell ämneskategori
Mikrobiologi
Forskningsämne
Naturvetenskap, Biomedicinsk vetenskap
Identifikatorer
urn:nbn:se:lnu:diva-16513 (URN)10.1186/1743-422X-8-473 (DOI)21999585 (PubMedID)2-s2.0-80054008339 (Scopus ID)
Tillgänglig från: 2012-01-03 Skapad: 2012-01-03 Senast uppdaterad: 2017-12-08Bibliografiskt granskad
5. Studies of Echovirus 5 interactions with the cell surface: Heparan sulfate mediates attachment to the host cell.
Öppna denna publikation i ny flik eller fönster >>Studies of Echovirus 5 interactions with the cell surface: Heparan sulfate mediates attachment to the host cell.
Visa övriga...
2010 (Engelska)Ingår i: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 151, nr 2, s. 170-176Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Infections caused by Echovirus 5 (E5), an enterovirus of the Picornaviridae family, have been associated with fever, rashes and sporadic cases of aseptic meningitis. To elucidate the receptor usage of this virus, the significance of a previously proposed integrin binding arginine-glycine-aspartic acid (RGD) motif found in the VP3 capsid protein was investigated, as well as the capacity of E5 to interact with heparan sulfate on the cell surface. Using the prototype strain E5 Noyce (E5N), an E5N mutant where the aspartic acid of the RGD motif has been substituted to a glutamic acid and clinical E5 isolates, the RGD motif of VP3 was found to be non-essential and hence not involved in integrin receptor binding. However, E5N and clinical E5 isolates interact with heparan sulfate at the cell surface, as demonstrated by virus replication inhibition assays using heparin and heparinase III, and studies of E5 interactions at the cell surface measured by real-time PCR analysis. In conclusion, E5 utilizes heparan sulfate as a cellular receptor, but the RGD motif of VP3 is not essential for E5 infectivity.

Nationell ämneskategori
Mikrobiologi inom det medicinska området
Forskningsämne
Biomedicinsk vetenskap, Virologi
Identifikatorer
urn:nbn:se:lnu:diva-7169 (URN)10.1016/j.virusres.2010.05.001 (DOI)20466025 (PubMedID)2-s2.0-77954219093 (Scopus ID)
Tillgänglig från: 2010-08-13 Skapad: 2010-08-13 Senast uppdaterad: 2018-01-12Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Personposter BETA

Israelsson, Stina

Sök vidare i DiVA

Av författaren/redaktören
Israelsson, Stina
Av organisationen
Institutionen för naturvetenskap, NV
Annan naturvetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetricpoäng

isbn
urn-nbn
Totalt: 451 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf