lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Framtagning av normalt referensintervall för RDW och retikulocyter på hematologiinstrumentet ADVIA 2120
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
2012 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Erythropoiesis is a complex and carefully controlled process which is mainly regulated by erythropoietin. Using flow cytometry cells in a blood sample can be analyzed one by one. ADVIA 2120 using flow cytometry for analysis of red blood cells (RBC) and reticulocytes. Red blood cell distribution width (RDW) is a component of the RBC method. RDW indicates size variation on erythrocytes and is measured as a percentage of the mean cell volume (MCV). By measuring the RDW anemia can be divided into makrocytic, microcytic or normocytic. Reticulocytes refract light more strongly than red blood cells because they contain some cellular debris. The number of reticulocytes in the peripheral blood reflects erythropoiesis in the bone marrow. 

The purpose of this project was that by approved methods to establish reference ranges for the parameters, RDW and reticulocyte counts.

The individuals who participated in the study, n=124, were considered healthy and had varying ethnicity. Reference subjects had not donated blood the last six months and participation in the study was voluntary.

The statistic evaluation includes examination of distribution, and reference ranges for both men and women were established using the 2.5:t and 97.5:t percentiles. The calculated reference interval for RDW was between 12.0 – 14.3% and the calculated reference interval for reticulocytes was between 38.0 – 112.8 x109 cells/L. The results showed a significant difference between males and females for the parameter reticulocytes was seen. The difference indicates that different reference ranges should be established for men and women (p<0,0001). No significant difference between males and females were seen for RDW (p=0,74) indicating that the same reference ranges can be used for both men and women. 

Place, publisher, year, edition, pages
2012. , p. 14
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:lnu:diva-21898OAI: oai:DiVA.org:lnu-21898DiVA, id: diva2:558720
External cooperation
Länssjukhuset i Kalmar
Subject / course
Biomedical Sciences
Educational program
Biomedical Laboratory Science Programme, 180 credits
Presentation
(Swedish)
Uppsok
Medicine
Supervisors
Examiners
Available from: 2012-10-08 Created: 2012-10-04 Last updated: 2012-10-08Bibliographically approved

Open Access in DiVA

fulltext(576 kB)3240 downloads
File information
File name FULLTEXT01.pdfFile size 576 kBChecksum SHA-512
f817d1815c67157d3af6509a4004e073ffa11a55be94c05f671274e4fc90a07592003ce6ec38cceb0f60dccd5014ebeed4b78734559b454edb0ccb787b55a41e
Type fulltextMimetype application/pdf

Other links

http://2012:BL10

Search in DiVA

By author/editor
Henriksson Karlsson, Jinnie
By organisation
School of Natural Sciences
Biomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 3241 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 246 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf