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Determination of Sulfite in Beer Based on Fluorescent Derivatives and Liquid Chromatographic Separation
Linnéuniversitetet, Fakultetsnämnden för naturvetenskap och teknik, Institutionen för naturvetenskap, NV.
University of Copenhagen, Denmark.
University of Copenhagen, Denmark.
University of Copenhagen, Denmark.
Vise andre og tillknytning
2012 (engelsk)Inngår i: Journal of the American Society of Brewing Chemists, ISSN 0361-0470, E-ISSN 1943-7854, Vol. 70, nr 4, s. 296-302Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo I followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrix-matched calibration curve is proposed. ThioGlo I was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

sted, utgiver, år, opplag, sider
2012. Vol. 70, nr 4, s. 296-302
Emneord [en]
Analysis, Beer, Matrix match, Sulfite, ThioGlo 1
HSV kategori
Forskningsprogram
Kemi, Organisk kemi
Identifikatorer
URN: urn:nbn:se:lnu:diva-24502DOI: 10.1094/ASBCJ-2012-1012-01ISI: 000312628600011Scopus ID: 2-s2.0-84871342213OAI: oai:DiVA.org:lnu-24502DiVA, id: diva2:607289
Tilgjengelig fra: 2013-02-22 Laget: 2013-02-22 Sist oppdatert: 2017-12-06bibliografisk kontrollert

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