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Campylobacter jejuni Actively Invades the Amoeba Acanthamoeba polyphaga and Survives within Non Digestive Vacuoles
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Uppsala University.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science.
Kalmar County Hospital ; Linköping University.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Uppsala University.ORCID iD: 0000-0002-4646-691x
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 11, article id e78873Article in journal (Refereed) Published
Abstract [en]

The Gram-negative bacterium Campylobacter jejuni is able to enter, survive and multiply within the free living amoeba Acanthamoeba polyphaga, but the molecular mechanisms behind these events are still unclear. We have studied the uptake and intracellular trafficking of viable and heat killed bacterial cells of the C. jejuni strain 81-176 in A. polyphaga. We found that viable bacteria associated with a substantially higher proportion of Acanthamoeba trophozoites than heat killed bacteria. Furthermore, the kinetics of internalization, the total number of internalized bacteria as well as the intracellular localization of internalized C. jejuni were dramatically influenced by bacterial viability. Viable bacteria were internalized at a high rate already after 1 h of co-incubation and were observed in small vacuoles tightly surrounding the bacteria. In contrast, internalization of heat killed C. jejuni was low at early time points and did not peak until 96 h. These cells were gathered in large spacious vacuoles that were part of the degradative pathway as determined by the uptake of fluorescently labeled dextran. The amount of heat killed bacteria internalized by A. polyphaga did never reach the maximal amount of internalized viable bacteria. These results suggest that the uptake and intracellular survival of C. jejuni in A. polyphaga is bacterially induced.

Place, publisher, year, edition, pages
2013. Vol. 8, no 11, article id e78873
National Category
Microbiology
Research subject
Ecology, Zoonotic Ecology
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URN: urn:nbn:se:lnu:diva-30994DOI: 10.1371/journal.pone.0078873ISI: 000326656200061Scopus ID: 2-s2.0-84892404372OAI: oai:DiVA.org:lnu-30994DiVA, id: diva2:676773
Available from: 2013-12-06 Created: 2013-12-06 Last updated: 2020-01-28Bibliographically approved

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Olofsson, JennyAxelsson Olsson, DianaOlsen, Björn

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