The study documented first time in Bangladesh of different locally practiced rope casting methods of Cattle-buffalo before slaughtering at local slaughter houses of six representative places of five different districts. The study also documented the most inhumane and primitive system of Cattle-buffalo transportation and handling in the abattoirs from both native and cross-border traded source. Animals were transported by driving on foot or in crowded truck for several days over harsh road without feed, water and adequate rest. We found that 89.06% animals were injured at sacral, ribs and shoulder area due to transportation in densely populated truck among 64 injured animals. It revealed that animals were slaughtered beside human toilet, road sides, unclean surface; and Dogs, Crows and Chickens easily entered at slaughter area. More than 21% animals were in very poor health condition and cachectic and 21.19% animals were injured and 11.25% animals had other clinical signs among 302 inspected animals. Diarrhoea was predominant other than injury (59.09%) and elevated body temp to the next (25%) and one pregnant animal also found to slaughter. We also detected six heavily injured and one highly exhausted lied animals just after unloading of truck by instant inspection at Cattle market. The research revealed that in Bangladesh Muslim Scholars permit conditional stunning before slaying but lack of facilities and information, humane slaughtering method is not practiced. Lack of implementation of the “Animal Slaughter and Meat Control Act”, absence or weak inspection of veterinarian increased unauthorized butcher-man and slaughter house; unhygienic, unsafe and lower quality meat production; animal welfare concern and zoonotic disease risk.