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Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Univ Montreal, Canada.ORCID iD: 0000-0001-6288-4022
Univ Montreal, Canada.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science.ORCID iD: 0000-0002-1903-760X
Univ Montreal, Canada.
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2017 (English)In: International Biodeterioration & Biodegradation, ISSN 0964-8305, E-ISSN 1879-0208, Vol. 116, 48-57 p.Article in journal (Refereed) Published
Abstract [en]

We investigated the potential for petroleum hydrocarbon biodegradation by 95 bacterial and 160 fungal strains isolated from a former petrochemical plant. We tested whether soil origin, culture media type, and strain taxonomy influenced the degradation of added petroleum hydrocarbon compounds. Preliminary screening was based on two colorimetric tests using 2,6-dichlorophenolindophenol and piodonitrotetrazolium indicators, to assess microbial strain tolerance to crude oil. Top-performing strains in these screening assays were then assessed for their ability to mineralize a mixture of four polycyclic aromatic hydrocarbons (PAH) for 49 days, using GC-MS quantification. The aerobic activity of these candidate strains was also assessed by respirometry over the first 24 days of incubation. On average, PAH degradation by microbial isolates from soil that was lightly, moderately, and highly contaminated with petroleum was equally efficient, and the type of culture medium used did not significantly impact mean biodegradation. Phylogenetic affiliation had a strong and significant effect on PAH biodegradation. Fungal isolates belonging to the group Sordariomycetes, and bacterial isolates belonging to the groups Actinobacteria, Betaproteobacteria, and Gammaproteobacteria showed high potential for PAH degradation. Three of the strains tested by GC-MS, Rhodococcus sp., Trichoderma tomentosum, and Fusarium oxysporum, significantly degraded all four PAH compounds in the mixture compared to the control. (C) 2016 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2017. Vol. 116, 48-57 p.
Keyword [en]
Bioremediation, Respirometry, Polycyclic aromatic hydrocarbon (PAH), Crude oil, Colorimetric tests
National Category
Environmental Sciences
Research subject
Natural Science, Environmental Science
Identifiers
URN: urn:nbn:se:lnu:diva-59808DOI: 10.1016/j.ibiod.2016.09.030ISI: 000390498700006OAI: oai:DiVA.org:lnu-59808DiVA: diva2:1065473
Available from: 2017-01-16 Created: 2017-01-13 Last updated: 2017-02-22Bibliographically approved
In thesis
1. Phytoremediation of soil contaminated with petroleum hydrocarbons and trace elements
Open this publication in new window or tab >>Phytoremediation of soil contaminated with petroleum hydrocarbons and trace elements
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The rapid urbanization and industrialization has led to an increase of disposal petroleum hydrocarbons (PHC) and trace elements (TE) into the environment. These pollutants are considered as the most toxic contaminants in the world due to their persistence in the environment, and the long range of toxicological effects for living beings.

Recent concerns regarding the environmental contamination have initiated the development of several remediation technologies, including physical, chemical, and biological approaches. In this thesis, gentle soil remediation options (GRO) were investigated at different scales for the reclamation of PHC and TE co-contaminated soil. In the first part of this thesis, laboratory experiments were performed to characterize PHC and TE contaminated soil as well as the indigenous microorganisms (bacteria and fungi) present inside these contaminated soil. It was found that the studied aged contaminated soil had a negative effect on earthworm’s development and L. sativum biomass. Moreover, a high respiration of microorganisms attributed to the transformation/ mineralization of organic matter or/and organic pollutants was observed. This presence of viable microorganisms suggested an adaptation of microorganisms to the contaminant. Further results showed that the long-term exposure of soil microorganisms to high PHC concentration and the type of isolation culture media did not influence the ability of isolates to effectively degrade PHC. However, phylogenic affiliation had a strong on PHC biodegradation. In the second part of this thesis, preliminary studies in greenhouse were assessed to investigate the ability of M. sativa assisted by compost in the greenhouse aided-phytoremediation of PHC and TE. It was found that compost incorporation into the soil promoted PHC degradation, M. sativa growth and survival, and phytoextraction of TE. Residual risk assessment after the phytoremediation trial also showed a positive effect of compost amendment on plant growth and earthworm development. Pilot scale ecopile experiment carried out in the third part of this thesis allow a reduction of up to 80% of PHC and 20% of metals after 17 months. This research demonstrated that M. sativa and H. annus were suitable for phytodegradation of PHC and phytoextraction of TE.  Results from this thesis are helpful for further full-scale phytoremediation studies. 

Place, publisher, year, edition, pages
Växjö: Linnaeus University Press, 2017. 212 p.
Series
Linnaeus University Dissertations, 279/2017
Keyword
Petroleum Hydrocarbon, Trace Elements, Gentle Soil Remediation Options, Polycyclic Aromatic Hydrocarbon, Respirometry, Ecopile, Bacteria, Fungi
National Category
Environmental Sciences
Research subject
Natural Science, Environmental Science
Identifiers
urn:nbn:se:lnu:diva-60839 (URN)978-91-88357-63-2 (ISBN)
Public defence
2017-03-10, N2007, Västergård, Kalmar, 14:30 (English)
Opponent
Supervisors
Available from: 2017-02-22 Created: 2017-02-22 Last updated: 2017-03-16Bibliographically approved

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