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Chemical and Genetic Diversity of Nodularia spumigena from the Baltic Sea
University of Gdansk, Poland.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (Ctr Ecol & Evolut Microbial Model Syst, EEMiS)
University of Gdansk, Poland.
University of Gdansk, Poland.
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2016 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 14, no 11, article id 209Article in journal (Refereed) Published
Abstract [en]

Nodularia spumigena is a toxic, filamentous cyanobacterium occurring in brackish waters worldwide, yet forms extensive recurrent blooms in the Baltic Sea. N. spumigena produces several classes of non-ribosomal peptides (NRPs) that are active against several key metabolic enzymes. Previously, strains from geographically distant regions showed distinct NRP metabolic profiles. In this work, conspecific diversity in N. spumigena was studied using chemical and genetic approaches. NRP profiles were determined in 25 N. spumigena strains isolated in different years and from different locations in the Baltic Sea using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Genetic diversity was assessed by targeting the phycocyanin intergenic spacer and flanking regions (cpcBA-IGS). Overall, 14 spumigins, 5 aeruginosins, 2 pseudaeruginosins, 2 nodularins, 36 anabaenopeptins, and one new cyanopeptolin-like peptide were identified among the strains. Seven anabaenopeptins were new structures; one cyanopeptolin-like peptide was discovered in N. spumigena for the first time. Based on NRP profiles and cpcBA-IGS sequences, the strains were grouped into two main clusters without apparent influence of year and location, indicating persistent presence of these two subpopulations in the Baltic Sea. This study is a major step in using chemical profiling to explore conspecific diversity with a higher resolution than with a sole genetic approach.

Place, publisher, year, edition, pages
MDPI AG , 2016. Vol. 14, no 11, article id 209
Keywords [en]
Nodularia spumigena, Baltic Sea, cyanobacteria, chemotaxonomy, non-ribosomal peptides, PC-IGS
National Category
Microbiology
Research subject
Ecology, Aquatic Ecology; Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-59813DOI: 10.3390/md14110209ISI: 000390097800016Scopus ID: 2-s2.0-84996555333OAI: oai:DiVA.org:lnu-59813DiVA, id: diva2:1065482
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EcoChangeAvailable from: 2017-01-16 Created: 2017-01-13 Last updated: 2018-06-08Bibliographically approved

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Bertos-Fortis, MireiaLegrand, Catherine

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