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High serum CXCL10 in Rickettsia conorii infection is endothelial cell mediated subsequent to whole blood activation.
Oslo University Hospital Rikshospitalet, Norway.
Hospital San Pedro-Center of Biomedical Research from La Rioja (CIBIR), Spain.
Oslo University Hospital Rikshospitalet, Norway ; University of Oslo, Norway.
Research Laboratory Nordland Hospital, Norway.
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2016 (English)In: Cytokine, ISSN 1043-4666, E-ISSN 1096-0023, Vol. 83, 269-274 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The pathophysiological hallmark of Rickettsia conorii (R. conorii) infection comprises infection of endothelial cells with perivascular infiltration of T-cells and macrophages. Although interferon (IFN)-γ-induced protein 10 (IP-10)/CXCL10 is induced during vascular inflammation, data on CXCL10 in R. conorii infection is scarce.

METHODS: Serum CXCL10 was analyzed in two cohorts of southern European patients with R. conorii infection using multiplex cytokine assays. The mechanism of R. conorii-induced CXCL10 release was examined ex vivo using human whole blood interacting with endothelial cells.

RESULTS: (i) At admission, R. conorii infected patients had excessively increased CXCL10 levels, similar in the Italian (n=32, ∼56-fold increase vs controls) and the Spanish cohort (n=38, ∼68-fold increase vs controls), followed by a marked decrease after recovery. The massive CXCL10 increase was selective since it was not accompanied with similar changes in other cytokines. (ii) Heat-inactivated R. conorii induced a marked CXCL10 increase when whole blood and endothelial cells were co-cultured. Even plasma obtained from R. conorii-exposed whole blood induced a marked CXCL10 release from endothelial cells, comparable to the levels found in serum of R. conorii-infected patients. Bacteria alone did not induce CXCL10 production in endothelial cells, macrophages or smooth muscle cells.

CONCLUSIONS: We show a massive and selective serum CXCL10 response in R. conorii-infected patients, likely reflecting release from infected endothelial cells characterized by infiltrating T cells and monocytes. The CXCL10 response could contribute to T-cell infiltration within the infected organ, but the pathologic consequences of CXCL10 in clinical R. conorii infection remain to be defined.

Place, publisher, year, edition, pages
2016. Vol. 83, 269-274 p.
National Category
Immunology in the medical area
Research subject
Biomedical Sciences, Immunology
Identifiers
URN: urn:nbn:se:lnu:diva-60954DOI: 10.1016/j.cyto.2016.05.006ISI: 000377228300036PubMedID: 27180202OAI: oai:DiVA.org:lnu-60954DiVA: diva2:1077195
Available from: 2017-02-26 Created: 2017-02-26 Last updated: 2017-03-20Bibliographically approved

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