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Silk matrices promote formation of insulin-secreting islet-like clusters
KTH Royal Institute of Technology.
Karolinska Institutet.
KTH Royal Institute of Technology.
Karolinska Institutet.
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2016 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 90, 50-61 p.Article in journal (Refereed) Published
Abstract [en]

Ex vivo expansion of endocrine cells constitutes an interesting alternative to be able to match the unmet need of transplantable pancreatic islets. However, endocrine cells become fragile once removed from their extracellular matrix (ECM) and typically become senescent and loose insulin expression during conventional 2D culture. Herein we develop a protocol where 3D silk matrices functionalized with ECM-derived motifs are used for generation of insulin-secreting islet-like clusters from mouse and human primary cells. The obtained clusters were shown to attain an islet-like spheroid shape and to maintain functional insulin release upon glucose stimulation in vitro. Furthermore, in vivo imaging of transplanted murine clusters showed engraftment with increasing vessel formation during time. There was no sign of cell death and the clusters maintained or increased in size throughout the period, thus suggesting a suitable cluster size for transplantation.

Place, publisher, year, edition, pages
2016. Vol. 90, 50-61 p.
Keyword [en]
Recombinant spider silk, Pancreatic islets, Islet-like clusters, Insulin release, In vivo imaging, Vascularization
National Category
Cell and Molecular Biology
Research subject
Natural Science
Identifiers
URN: urn:nbn:se:lnu:diva-61320DOI: 10.1016/j.biomaterials.2016.03.006OAI: oai:DiVA.org:lnu-61320DiVA: diva2:1081159
Available from: 2017-03-13 Created: 2017-03-13 Last updated: 2017-03-20Bibliographically approved

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Johansson, Ulrika
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CiteExportLink to record
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Citation style
  • apa
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