lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Evaluation of a radioprotein-binding assay (RPBA) for folate analysis in berries and milk
Swedish University of Agricultural Sciences.
Swedish University of Agricultural Sciences.
Swedish University of Agricultural Sciences.ORCID iD: 0000-0003-0387-4312
Swedish University of Agricultural Sciences.
2002 (English)In: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 79, no 4, 525-534 p.Article in journal (Refereed) Published
Abstract [en]

This study aimed to optimise a commercial radioprotein-binding assay (RPBA), routinely used for clinical samples, for folate quantification in foods containing mainly 5-CH3-H(4)folate. The assay was modified using external calibration with 5-CH3-H(4)folate in a lower concentration range diluted in food extraction buffer, rather than the buffer with human serum albumin (HSA) provided by the kit. We evaluated the modified RBPA on some selected food products; milk, whey powder, rose hips, strawberries and European certified reference materials (CRM 421, 485) and the adjustments did not affect the assay negatively. Performance parameters included control of selectivity, absence of matrix effects, recoveries of 94-113%, precision of 1-6 CV% (intra-assay) and 5-9 CV% (inter-assay). Folate concentrations in berries and milk, obtained by the modified RPBA were also compared with other quantification methods such as HPLC and MA. The optimised RPBA was found to be a quick and inexpensive complement to HPLC methods, reliable for folate quantification in foods such as milk and berries that contain mainly 5-CH3-H(4)folate. (C) 2002 Elsevier Science Ltd. All rights reserved.

Place, publisher, year, edition, pages
2002. Vol. 79, no 4, 525-534 p.
Keyword [en]
5-CH3-H(4)folate, radioprotein-binding assay, quality control, method evaluation, food analysis, berries, milk
National Category
Food Science
Research subject
Natural Science
Identifiers
URN: urn:nbn:se:lnu:diva-61261DOI: 10.1016/S0308-8146(02)00392-8ISI: 000179308300017OAI: oai:DiVA.org:lnu-61261DiVA: diva2:1081603
Available from: 2017-03-14 Created: 2017-03-14 Last updated: 2017-03-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Witthöft, Cornelia M.
In the same journal
Food Chemistry
Food Science

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 2 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf