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Multi-omics reveal the lifestyle of the acidophilic, mineral-oxidizing model species Leptospirillum ferriphilumT
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. (Centre for Ecology and Evolution in Microbial Model Systems EEMiS)ORCID iD: 0000-0003-0021-2452
University of Luxembourg, Luxembourg.
Universität Duisburg-Essen, Germany.
Ruhr Universität Bochum, Germany.
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2018 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 4, no 3, article id UNSP e02091-17Article in journal (Refereed) Published
Abstract [en]

Leptospirillum ferriphilum plays a major role in acidic, metal rich environments where it represents one of the most prevalent iron oxidizers. These milieus include acid rock and mine drainage as well as biomining operations. Despite its perceived importance, no complete genome sequence of this model species' type strain is available, limiting the possibilities to investigate the strategies and adaptations Leptospirillum ferriphilumT applies to survive and compete in its niche. This study presents a complete, circular genome of Leptospirillum ferriphilumT DSM 14647 obtained by PacBio SMRT long read sequencing for use as a high quality reference. Analysis of the functionally annotated genome, mRNA transcripts, and protein concentrations revealed a previously undiscovered nitrogenase cluster for atmospheric nitrogen fixation and elucidated metabolic systems taking part in energy conservation, carbon fixation, pH homeostasis, heavy metal tolerance, oxidative stress response, chemotaxis and motility, quorum sensing, and biofilm formation. Additionally, mRNA transcript counts and protein concentrations were compared between cells grown in continuous culture using ferrous iron as substrate and bioleaching cultures containing chalcopyrite (CuFeS2). Leptospirillum ferriphilumT adaptations to growth on chalcopyrite included a possibly enhanced production of reducing power, reduced carbon dioxide fixation, as well as elevated RNA transcripts and proteins involved in heavy metal resistance, with special emphasis on copper efflux systems. Finally, expression and translation of genes responsible for chemotaxis and motility were enhanced.

Place, publisher, year, edition, pages
American society for microbiology , 2018. Vol. 4, no 3, article id UNSP e02091-17
National Category
Microbiology
Research subject
Ecology, Microbiology; Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-69199DOI: 10.1128/AEM.02091-17ISI: 000423770000018OAI: oai:DiVA.org:lnu-69199DiVA: diva2:1165354
Available from: 2017-12-13 Created: 2017-12-13 Last updated: 2018-02-15Bibliographically approved

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Christel, StephanBuetti-Dinh, AntoineDopson, Mark

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