lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A model to study complement involvement in experimental retinal degeneration.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. (Linnaeus Center of Biomaterials Chemistry)
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. (Linnaeus Center of Biomaterials Chemistry)
Karolinska institutet.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Uppsala university. (Linnaeus Center of Biomaterials Chemistry)ORCID iD: 0000-0001-7888-1571
Show others and affiliations
2018 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 123, no 1, p. 28-42Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The complement system (CS) plays a role in the pathogenesis of a number of ocular diseases, including diabetic retinopathy (DR), glaucoma, uveitis, and age-related macular degeneration (AMD). Given that many of the complex eye-related degenerative diseases have limited treatment opportunities, we aimed to mimic the in vivo retinal degenerative process by developing a relevant co-culture system.

METHOD AND MATERIALS: The adult porcine retina was co-cultured with the spontaneously arising human retinal pigment epithelial cells-19 (ARPE-19).

RESULTS: Inflammatory activity was found after culture and included migrating microglial cells, gliosis, cell death, and CS activation (demonstrated by a minor increase in the secreted anaphylotoxin C3a in co-culture). CS components, including C1q, C3, C4, soluble C5b-9, and the C5a receptor, were expressed in the retina and/or ARPE cells after culture. C1q, C3, and CS regulators such as C4 binding protein (C4BP), factor H (CFH), and factor I (CFI) were secreted after culture.

DISCUSSION: Thus, our research indicates that this co-culturing system may be useful for investigations of the CS and its involvement in experimental neurodegenerative diseases.

Place, publisher, year, edition, pages
Taylor & Francis, 2018. Vol. 123, no 1, p. 28-42
Keywords [en]
AMD, RPE, complement system, ocular diseases, retina
National Category
Immunology in the medical area
Research subject
Biomedical Sciences, Immunology
Identifiers
URN: urn:nbn:se:lnu:diva-71489DOI: 10.1080/03009734.2018.1431744ISI: 000428060300004PubMedID: 29436895OAI: oai:DiVA.org:lnu-71489DiVA, id: diva2:1189660
Available from: 2018-03-12 Created: 2018-03-12 Last updated: 2018-04-19Bibliographically approved

Open Access in DiVA

fulltext(2777 kB)18 downloads
File information
File name FULLTEXT02.pdfFile size 2777 kBChecksum SHA-512
b730b1309369bc0ba9d4b2a0f938bf5152afc86e828c0b9143019088021960b840c6aefb37f4876629fabe63f25ffad10e85db2fea890fba730c2ad557fd9b6c
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Mohlin, CamillaSandholm, KerstinNilsson Ekdahl, Kristina

Search in DiVA

By author/editor
Mohlin, CamillaSandholm, KerstinNilsson Ekdahl, Kristina
By organisation
Department of Chemistry and Biomedical Sciences
In the same journal
Upsala Journal of Medical Sciences
Immunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 26 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 102 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf