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Effect of U(VI) aqueous speciation on the binding of uranium by the cell surface of Rhodotorula mucilaginosa, a natural yeast isolate from bentonites
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Univ Granada, Spain. (Ctr Ecol & Evolut Microbial Model Syst EEMiS)
Univ Sheffield, UK.
Helmholtz Zentrum Dresden Rossendorf, Germany.
Synchrotron SOLEIL, France.
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2018 (English)In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 199, p. 351-360Article in journal (Refereed) Published
Abstract [en]

This study presents the effect of aqueous uranium speciation (U-hydroxides and U-hydroxo-carbonates) on the interaction of this radionuclide with the cells of the yeast Rhodotorula mucigilanosa BII-R8. This strain was isolated from Spanish bentonites considered as reference materials for the engineered barrier components of the future deep geological repository of radioactive waste. X-ray absorption and infrared spectroscopy showed that the aqueous uranium speciation has no effect on the uranium binding process by this yeast strain. The cells bind mobile uranium species (U-hydroxides and U-hydroxo-carbonates) from solution via a time-dependent process initiated by the adsorption of uranium species to carboxyl groups. This leads to the subsequent involvement of organic phosphate groups forming uranium complexes with a local coordination similar to that of the uranyl mineral phase meta-autunite. Scanning transmission electron microscopy with high angle annular dark field analysis showed uranium accumulations at the cell surface associated with phosphorus containing ligands. Moreover, the effect of uranium mobile species on the cell viability and metabolic activity was examined by means of flow cytometry techniques, revealing that the cell metabolism is more affected by higher concentrations of uranium than the cell viability. The results obtained in this work provide new insights on the interaction of uranium with bentonite natural yeast from genus Rhodotorula under deep geological repository relevant conditions. (C) 2018 The Authors. Published by Elsevier Ltd.

Place, publisher, year, edition, pages
Pergamon Press, 2018. Vol. 199, p. 351-360
Keywords [en]
Uranium biosorption, Cell surface, Speciation, Yeast Rhodotorula, Spectroscopy, Microscopy
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-73122DOI: 10.1016/j.chemosphere.2018.02.055ISI: 000428973200043PubMedID: 29453061OAI: oai:DiVA.org:lnu-73122DiVA, id: diva2:1199433
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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Lopez-Fernandez, Margarita

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