PurposeTo quantify light return from retinal vessels for oxygenation status. To use a low-cost Digital Light Ophthalmoscope (DLO) based on a novel structured light pattern Digital Light Projector for quantitative retinal imaging.  MethodsFive normal subjects of ages 27-63 and various eye colors were imaged without mydriasis. The DLO produced a progressively scanning 6 pixel wide stripe, 96 stripe illumination pattern (85 μm stripe width at retina) with LEDs at 635 +/- 25nm (Red) and 535 +/- 70nm (Green) with a constant fixation target location across tests. The illumination was synchronized to a 13.2 Hz rolling shutter CMOS sensor (11 μm resolution at retina). To collect the light return from the retina at varying light multiply scattered levels, aperture offset was varied in position with respect to the illumination centerline: dark-field mode was obtained with large offsets and confocal mode had small offsets, where offset was varied from -517 μm (row start 30) to 1353 μm (row start 200). Multiple images of 12 bit dynamic range were captured in sequence, aligned with translational cross correlation, then time averaged to reduce noise. The artery and vein gray scale intensity level was measured at vessel centers for: 1) both vessels over background retina and 2) artery over the optic disc and vein over background. Mean-to-mean intensity ratios were then compared.  ResultsWith an aperture width of 704 μm, intensity varied by 2.60 bits (a factor of 4.5) for each subject per condition: red, green, artery, and vein, 3.39 bits if same subject and condition (2.3% Red A/V ratio CoV σ/μ), and by 5.07 bits over all tests. Yet, the ratio (Red/Green Artery)/(Red/Green Vein) was close to unity across offsets and linear fits had an R^2 regression of, for case 1: 0.72, 0.06, 0.25, 0.18, and 0.49, and case 2: 0.0005, 0.89, 0.75, 0.50, and 0.18. The Artery/Vein ratio trend slopes, intercept points, and inversions varied between subjects, i.e. contrast reversals were observed.  ConclusionsThe DLO can be utilized electronically to effectively perform quantitative fundus imaging at both Red and Green wavelengths, which is a step towards performing oximetry. 77 is zero offset