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ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain
Technion, Israel.
Technion, Israel.
Technion, Israel.
Technion, Israel.
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, no 1, article id 11577Article in journal (Refereed) Published
Abstract [en]

The role of the actin cytoskeleton in relation to mitochondria function and dynamics is only recently beginning to be recognized. Myo19 is an actin-based motor that is bound to the outer mitochondrial membrane and promotes the localization of mitochondria to filopodia in response to glucose starvation. However, how glucose starvation induces mitochondria localization to filopodia, what are the dynamics of this process and which enzymatic adaptation allows the translocation of mitochondria to filopodia are not known. Here we show that reactive oxygen species (ROS) mimic and mediate the glucose starvation induced phenotype. In addition, time-lapse fluorescent microscopy reveals that ROS-induced Myo19 motility is a highly dynamic process which is coupled to filopodia elongation and retraction. Interestingly, Myo19 motility is inhibited by back-to-consensus-mutation of a unique residue of class XIX myosins in the motor domain. Kinetic analysis of the purified mutant Myo19 motor domain reveals that the duty ratio (time spent strongly bound to actin) is highly compromised in comparison to that of the WT motor domain, indicating that Myo19 unique motor properties are necessary to propel mitochondria to filopodia tips. In summary, our study demonstrates the contribution of actin-based motility to the mitochondrial localization to filopodia by specific cellular cues.

Place, publisher, year, edition, pages
2017. Vol. 7, no 1, article id 11577
National Category
Biochemistry and Molecular Biology Biophysics Cell Biology
Research subject
Natural Science, Cell and Organism Biology
Identifiers
URN: urn:nbn:se:lnu:diva-75908DOI: 10.1038/s41598-017-11002-9OAI: oai:DiVA.org:lnu-75908DiVA, id: diva2:1218514
Available from: 2018-06-14 Created: 2018-06-14 Last updated: 2018-06-15Bibliographically approved

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CiteExportLink to record
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  • apa
  • harvard1
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  • modern-language-association-8th-edition
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  • de-DE
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  • Other locale
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Output format
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