lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cell electrofusion using nanosecond electric pulses
University of Ljubljana, Slovenia.
University of Ljubljana, Slovenia.
University of Ljubljana, Slovenia.
University of Ljubljana, Slovenia.
Show others and affiliations
2013 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 3, article id 3382Article in journal (Refereed) Published
Abstract [en]

Electrofusion is an efficient method for fusing cells using short-duration high-voltage electric pulses. However, electrofusion yields are very low when fusion partner cells differ considerably in their size, since the extent of electroporation (consequently membrane fusogenic state) with conventionally used microsecond pulses depends proportionally on the cell radius. We here propose a new and innovative approach to fuse cells with shorter, nanosecond (ns) pulses. Using numerical calculations we demonstrate that ns pulses can induce selective electroporation of the contact areas between cells (i.e. the target areas), regardless of the cell size. We then confirm experimentally on B16-F1 and CHO cell lines that electrofusion of cells with either equal or different size by using ns pulses is indeed feasible. Based on our results we expect that ns pulses can improve fusion yields in electrofusion of cells with different size, such as myeloma cells and B lymphocytes in hybridoma technology.

Place, publisher, year, edition, pages
Macmillan Publishers Ltd., 2013. Vol. 3, article id 3382
National Category
Medical Biotechnology Medical Equipment Engineering Other Medical Engineering Other Medical Biotechnology
Research subject
Chemistry, Biotechnology
Identifiers
URN: urn:nbn:se:lnu:diva-75929DOI: 10.1038/srep03382OAI: oai:DiVA.org:lnu-75929DiVA, id: diva2:1218598
Available from: 2018-06-14 Created: 2018-06-14 Last updated: 2018-06-15Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records BETA

Ušaj, Marko

Search in DiVA

By author/editor
Ušaj, Marko
In the same journal
Scientific Reports
Medical BiotechnologyMedical Equipment EngineeringOther Medical EngineeringOther Medical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 105 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf