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Modified Adherence Method (MAM) for Electrofusion of Anchorage-Dependent Cells
University of Ljubljana, Slovenia.
University of Ljubljana, Slovenia.
2015 (English)In: Cell Fusion: Overviews and Methods, New York, NY: Humana Press, 2015, p. 203-216Chapter in book (Refereed)
Abstract [en]

The artificially induced cell fusion is a useful experimental tool in biology, biotechnology and medicine. The electrofusion is a physical method for cell fusion that applies high-voltage electric pulses. The use of electric pulses causes cell membrane structural changes which bring the cell membrane in the so-called fusogenic state. When such fusogenic membranes are in close contact cell fusion takes place. Physical contact between fusion partners can be achieved by various methods and one of them is modified adherence method (MAM) described in detail here on B16-F1 cell line. The method is based on the fact that living cells form contacts in confluent culture. However, instead of using confluent cell culture, in modified adherence method cells are plated in suitable concentration and allowed to form contacts for only short predetermined period of time. During that time the cells are only slightly attached to the dish surface maintaining the spherical shape. Observed high fusion yields up to 50 % obtained by MAM in situ by dual-color fluorescence microscopy are among the highest in field of electrofusion. The method can be readily adapted to other anchorage-dependent cell lines.

Place, publisher, year, edition, pages
New York, NY: Humana Press, 2015. p. 203-216
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1313
National Category
Bioprocess Technology Medical Biotechnology Other Medical Engineering
Identifiers
URN: urn:nbn:se:lnu:diva-75931DOI: 10.1007/978-1-4939-2703-6_15ISBN: 978-1-4939-2703-6 (electronic)ISBN: 978-1-4939-2702-9 (print)OAI: oai:DiVA.org:lnu-75931DiVA, id: diva2:1218608
Available from: 2018-06-14 Created: 2018-06-14 Last updated: 2018-06-15Bibliographically approved

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Publisher's full texthttps://link.springer.com/protocol/10.1007%2F978-1-4939-2703-6_15

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Ušaj, Marko

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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
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