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Chloramphenicol Derivatives with Antibacterial Activity Identified by Functional Metagenomics
Linnaeus University. (universityof mississppi)ORCID iD: 0000-0002-8849-9883
(Auburn university)
(auburn university)
(university of mississippi)
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2018 (English)In: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025Article in journal (Refereed) Published
Abstract [en]

A functional metagenomic approach identified novel and diverse soil-derived DNAs encoding inhibitors to methicillinresistant Staphylococcus aureus (MRSA). A metagenomic DNA soil library containing 19 200 recombinant Escherichia coli BAC clones with 100 Kb average insert size was screened for antibiotic activity. Twenty-seven clones inhibited MRSA, seven of which were found by LC-MS to possess modified chloramphenicol (Cm) derivatives, including three new compounds whose structures were established as 1-acetyl-3-propanoylchloramphenicol, 1-acetyl-3-butanoylchloramphenicol, and 3-butanoyl-1-propanoylchloramphenicol. Cm was used as the selectable antibiotic for cloning, suggesting that heterologously expressed enzymes resulted in derivatization of Cm into new chemical entities with biological activity. An esterase was found to be responsible for the enzymatic regeneration of Cm, and the gene trfA responsible for plasmid copy induction was found to be responsible for inducing antibacterial activity in some clones. Six additional acylchloramphenicols were synthesized for structure and antibacterial activity relationship studies, with 1-p-nitrobenzoylchloramphenicol the most active against Mycobacterium intracellulare and Mycobacterium tuberculosis, with MICs of 12.5 and 50.0 μg/mL, respectively.

Place, publisher, year, edition, pages
USA: American Chemical Society (ACS), 2018.
National Category
Natural Sciences
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-76079DOI: 10.1021/acs.jnatprod.7b00903OAI: oai:DiVA.org:lnu-76079DiVA, id: diva2:1219811
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Idnetification of anti-microbial agents from metagenomic clonesAvailable from: 2018-06-18 Created: 2018-06-18 Last updated: 2018-06-18

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CiteExportLink to record
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