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Chloramphenicol Derivatives with Antibacterial Activity Identified by Functional Metagenomics
Auburn Univ, USA.
Univ Mississippi, USA.ORCID iD: 0000-0002-8849-9883
Auburn Univ, USA.
Univ Mississippi, USA.
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2018 (English)In: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025, Vol. 81, no 6, p. 1321-1332Article in journal (Refereed) Published
Abstract [en]

A functional metagenomic approach identified novel and diverse soil-derived DNAs encoding inhibitors to methicillin-resistant Staphylococcus aureus (MRSA). A metagenomic DNA soil library containing 19 200 recombinant Escherichia coli BAC clones with 100 Kb average insert size was screened for antibiotic activity. Twenty-seven clones inhibited MRSA, seven of which were found by LC-MS to possess modified chloramphenicol (Cm) derivatives, including three new compounds whose structures were established as 1-acetyl-3-propanoylchloramphenicol, 1-acety1-3-butanoyl-chloramphenicol, and 3-butanoyl-1-propanoylchloramphenicol. Cm was used as the selectable antibiotic for cloning, suggesting that heterologously expressed enzymes resulted in derivatization of Cm into new chemical entities with biological activity. An esterase was found to be responsible for the enzymatic regeneration of Cm, and the gene trfA responsible for plasmid copy induction was found to be responsible for inducing antibacterial activity in some clones. Six additional acylchloramphenicols were synthesized for structure and antibacterial activity relationship studies, with 1-p-nitrobenzoylchloramphenicol the most active against Mycobacterium intracellulare and Mycobacterium tuberculosis, with MICs of 12.5 and 50.0 mu g/mL, respectively.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018. Vol. 81, no 6, p. 1321-1332
National Category
Microbiology
Research subject
Ecology, Microbiology; Chemistry, Biotechnology
Identifiers
URN: urn:nbn:se:lnu:diva-76968DOI: 10.1021/acs.jnatprod.7b00903ISI: 000436527100003PubMedID: 29897754OAI: oai:DiVA.org:lnu-76968DiVA, id: diva2:1233962
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Identification of anti-microbial agents from metagenomic clonesAvailable from: 2018-07-20 Created: 2018-07-20 Last updated: 2018-07-20Bibliographically approved

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Ganji, Suresh

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