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A complete workflow for the differentiation and the dissociation of hiPSC-derived cardiospheres
Fraunhofer Inst Biomed Engn, Germany.
Fraunhofer Inst Biomed Engn, Germany;Tech Univ Munich, Germany.
Fraunhofer Inst Biomed Engn, Germany.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Fraunhofer Inst Biomed Engn, Germany.ORCID iD: 0000-0003-4835-0598
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2018 (English)In: Stem Cell Research, ISSN 1873-5061, E-ISSN 1876-7753, Vol. 32, p. 65-72Article in journal (Refereed) Published
Abstract [en]

Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) are an invaluable tool for both basic and translational cardiovascular research. The potential that these cells hold for therapy, disease modeling and drug discovery is hampered by several bottlenecks that currently limit both the yield and the efficiency of cardiac induction. Here, we present a complete workflow for the production of ready-to-use hiPSC-CMs in a dynamic suspension bioreactor. This includes the efficient and highly reproducible differentiation of hiPSCs into cardiospheres, which display enhanced physiological maturation compared to static 3D induction in hanging drops, and a novel papain-based dissociation method that offers higher yield and viability than the broadly used dissociation reagents TrypLE and Accutase. Molecular and functional analyses of the cardiomyocytes reseeded after dissociation confirmed both the identity and the functionality of the cells, which can be used in down-stream applications, either as monolayers or spheroids.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 32, p. 65-72
Keywords [en]
hiPSCs, Cardiomyocytes, Cardiac induction, 3D bioreactor, Papain dissociation
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-78614DOI: 10.1016/j.scr.2018.08.015ISI: 000447301200010PubMedID: 30218895Scopus ID: 2-s2.0-85053209652OAI: oai:DiVA.org:lnu-78614DiVA, id: diva2:1260280
Available from: 2018-11-01 Created: 2018-11-01 Last updated: 2019-08-29Bibliographically approved

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Salhotra, Aseem

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  • apa
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