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Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. (Linnaeus Ctr Biomat Chem, BMC)
Malmö University.
Malmö University.ORCID iD: 0000-0001-9460-0936
Univ Leicester, UK.
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2019 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 58, no 3, p. 727-730Article in journal (Refereed) Published
Abstract [en]

We report an approach integrating the synthesis of protein-imprinted nanogels ("plastic antibodies") with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino-functionalized solgel-coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high-dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein-conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited K-d<10 pM for their respective target protein and low cross-reactivity with five reference proteins. This agrees with affinities reported for solid-phase-synthesized nanogels prepared using low-surface-area glass-bead supports. This approach simplifies the development and production of plastic antibodies and offers direct access to a practical bioassay.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2019. Vol. 58, no 3, p. 727-730
Keywords [en]
competitive assays, molecularly imprinted polymers, nanogel synthesis, plastic antibodies
National Category
Organic Chemistry
Research subject
Chemistry, Organic Chemistry
Identifiers
URN: urn:nbn:se:lnu:diva-79846DOI: 10.1002/anie.201805772ISI: 000455033700009PubMedID: 30308085Scopus ID: 2-s2.0-85056479757OAI: oai:DiVA.org:lnu-79846DiVA, id: diva2:1282110
Funder
Knowledge Foundation, 20150086Knowledge Foundation, 20170059Swedish Research Council, 2014-4573
Note

Also funded by: Marie Sklodowska-Curie Actions (H2020-MSCA-ITN-2016)  -  722171-Biocapture

Available from: 2019-01-24 Created: 2019-01-24 Last updated: 2019-08-29Bibliographically approved

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Mahajan, RashmiNicholls, Ian A.

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Mahajan, RashmiShinde, SudhirkumarNicholls, Ian A.Sellergren, Börje
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