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UCYN-A3, a newly characterized open ocean sublineage of the symbiotic N2-fixing cyanobacterium Candidatus Atelocyanobacterium thalassa
Univ Calif Santa Cruz, USA;CSIC, Spain.ORCID iD: 0000-0001-8431-9278
Univ Calif Santa Cruz, USA;Univ Cordoba, Spain.
Univ Calif Santa Cruz, USA.
CSIC, Spain.
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2019 (English)In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 21, no 1, p. 111-124Article in journal (Refereed) Published
Abstract [en]

The symbiotic unicellular cyanobacterium Candidatus Atelocyanobacterium thalassa (UCYN-A) is one of the most abundant and widespread nitrogen (N-2)-fixing cyanobacteria in the ocean. Although it remains uncultivated, multiple sublineages have been detected based on partial nitrogenase (nifH) gene sequences, including the four most commonly detected sublineages UCYN-A1, UCYN-A2, UCYN-A3 and UCYN-A4. However, very little is known about UCYN-A3 beyond the nifH sequences from nifH gene diversity surveys. In this study, single cell sorting, DNA sequencing, qPCR and CARD-FISH assays revealed discrepancies involving the identification of sublineages, which led to new information on the diversity of the UCYN-A symbiosis. 16S rRNA and nifH gene sequencing on single sorted cells allowed us to identify the 16S rRNA gene of the uncharacterized UCYN-A3 sublineage. We designed new CARD-FISH probes that allowed us to distinguish and observe UCYN-A2 in a coastal location (SIO Pier; San Diego) and UCYN-A3 in an open ocean location (Station ALOHA; Hawaii). Moreover, we reconstructed about 13% of the UCYN-A3 genome from Tara Oceans metagenomic data. Finally, our findings unveil the UCYN-A3 symbiosis in open ocean waters suggesting that the different UCYN-A sublineages are distributed along different size fractions of the plankton defined by the cell-size ranges of their prymnesiophyte hosts.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2019. Vol. 21, no 1, p. 111-124
National Category
Microbiology Ecology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-80287DOI: 10.1111/1462-2920.14429ISI: 000456278900008PubMedID: 30255541Scopus ID: 2-s2.0-85056280294OAI: oai:DiVA.org:lnu-80287DiVA, id: diva2:1287042
Available from: 2019-02-08 Created: 2019-02-08 Last updated: 2019-08-29Bibliographically approved

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Farnelid, Hanna

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