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The Effect of Lipid Antioxidant α-Tocopherol on Cell Viability and Electrofusion Yield of B16-F1 Cells In Vitro
University of Ljubljana, Slovenia.
University of Ljubljana, Slovenia.
University of Ljubljana, Slovenia.
2019 (English)In: The Journal of Membrane Biology, ISSN 0022-2631, Vol. 252, no 1, p. 105-114Article in journal (Refereed) Published
Abstract [en]

Induced cell fusion is a powerful method for production of hybridoma in biotechnology and cell vaccines in medical applications. Among different alternatives, physical methods have an advantage, as they do not require any additives. Among them electrofusion, an electroporation-based cell fusion method holds a great promise. Electric pulses cause cell membrane permeabilization and due to pore formation bring cell membrane into the fusogenic state. At the same time, however, they compromise cell viability. We used a train of 8 × 100 µs electric pulses, delivered at 1 Hz with strengths ranging from 400 to 1600 V/cm. We evaluated electrofusion efficiency by dual color microscopy. We determined cell viability, because during electroporation reactive oxygen species are generated affecting cell survival. The novelty of our study is evaluation of the effect of lipid antioxidant α-tocopherol on cell fusion yield and cell viability on mouse B16-F1 cells. Pretreatment with α-tocopherol slowed down dynamic of cell fusion shortly after electroporation. Twenty-four hours later, fusion yields between α-tocopherol treated and untreated cells were comparable. The viability of α-tocopherol pretreated cells was drastically improved. Pretreatment of cells with α-tocopherol improved whole electrofusion process by more than 60%. We believe that α-tocopherol holds great promise to become an important agent to improve cell electrofusion method.

Place, publisher, year, edition, pages
Springer, 2019. Vol. 252, no 1, p. 105-114
Keywords [en]
Electrofusion, Vitamin E, In vitro
National Category
Cell Biology Other Medical Engineering Other Electrical Engineering, Electronic Engineering, Information Engineering
Research subject
Natural Science, Biomedical Sciences; Chemistry, Biotechnology; Natural Science, Cell and Organism Biology
Identifiers
URN: urn:nbn:se:lnu:diva-81830DOI: 10.1007/s00232-019-00059-4OAI: oai:DiVA.org:lnu-81830DiVA, id: diva2:1304017
Available from: 2019-04-11 Created: 2019-04-11 Last updated: 2019-05-06Bibliographically approved

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Ušaj, Marko

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CiteExportLink to record
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  • apa
  • harvard1
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